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Detection and identification of Candidatus liberobacter asiaticum
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GB/T 29393-2012
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PDF similar to GB/T 29393-2012
Basic data | Standard ID | GB/T 29393-2012 (GB/T29393-2012) | | Description (Translated English) | Detection and identification of Candidatus liberobacter asiaticum | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 9,980 | | Regulation (derived from) | National Standards Bulletin 2012 No. 42 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the citrus Huanglongbing pathogen Asian species Candidatus liberobacter asiaticum jagoueix et al. Detection and identification methods. This standard applies to plant quarantine during the course of the Asian species of Citrus Huan | GB/T 29393-2012: Detection and identification of Candidatus liberobacter asiaticum---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Candidatus liberobacter asiaticum
ICS 65.020.01
B16
National Standards of People's Republic of China
Quarantine detection and identification of bacteria huanglongbing
Issued on. 2012-12-31
2013-03-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. National Agricultural Technology Extension Service Center, Plant Protection and Quarantine Bureau of Zhejiang Province.
The main drafters of this standard. WU Li-feng, Yan iron, Lin Yunbiao, item Yu, Zhu Jing whole, Julie.
Quarantine detection and identification of bacteria huanglongbing
1 Scope
This standard specifies the Asian citrus Huanglong bacteria species Candidatusliberobacterasiaticumjagoueixetal. Detection and Identification
Setting Method.
This standard applies to plant quarantine process for the detection and identification of bacteria huanglongbing Asian species.
2 Basic information of germs
Chinese name. citrus Huanglong germs.
Name. Candidatusliberobacterasiaticumjagoueixetal.
Disease English. CitrusHuanglongbingHLB.
The case of thin-walled bacteria door Gracilicutes, phloem spp Liberobacter.
3 PRINCIPLE OF THE METHOD
According citrus citrus plants Huanglong fungal infection onset symptoms typical field test; by grafting or by citrus psyllids (Diaphorina
citri) after the transfer of disease, according to the onset of symptoms woody plant indicator identification; polymerase chain reaction (PolymeraseChainReaction, PCR) detection
Measurement Technology for huanglongbing bacteria in vitro amplification of DNA obtained rapid and effective identification results.
4 instruments and appliances
UV transilluminator or gel imaging systems, centrifuges, nucleic acid electrophoresis, pH meter, pipettes. 10μL, 20μL, 100μL,
1000μL, PCR instrument, water bath, ultra-pure water systems, electronic scales.
5 Reagents and materials
TAE buffer, tris (hydroxymethyl) aminomethane - HCl (Tris-HCl), ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulfate
(SDS), sodium acetate (NaAc), Taq enzyme, 10 × PCR buffer, 25mmol/L magnesium ion (Mg2), 20mmol/L Deoxynucleotide triphosphate
Phosphoric acid (dNTP), 5μLDNA fluorescent dye (GV), agarose, bromophenol blue, potassium permanganate, trichloromethane (chloroform), iso-amyl alcohol, isopropyl alcohol,
Ethanol.
6 Symptom Recognition
The main identification is based on Huanglongbing field event of the "type blade mottled yellow" and "yellow shoot." Vane type mottled yellow to green blade that is
After the main, lateral veins near the leaf base and start yellow, yellow green and yellow partial extension forming the uneven distribution of mottled, then can the whole leaf yellow
Of. Yellow tip that is in the early stages, a small part of the shoot green canopy foliage yellow sheet. Some results of citrus fruit from the tree was "red fruit nose." in
Huanglong citrus seedling disease wards meet the above typical symptoms of diseased trees can be directly identified as citrus greening disease.
Difficult to conclude suspected diseased seedlings, diseased trees, the choice of one or several detection methods for identification depending on the circumstances.
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