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GB/T 29396-2012: Detection and identification of Burkholderia glumae (Kurita and Tabei) Urakami et al.
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Detection and identification of Burkholderia glumae (Kurita and Tabei) Urakami et al.
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GB/T 29396-2012
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Basic data | Standard ID | GB/T 29396-2012 (GB/T29396-2012) | | Description (Translated English) | Detection and identification of Burkholderia glumae (Kurita and Tabei) Urakami et al. | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 19,172 | | Quoted Standard | GB 15569-1995 | | Regulation (derived from) | National Standards Bulletin 2012 No. 42 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the paddy rice seeds and other materials Valley rice bacterial blight (Burkholderia glumae) Identification of plant quarantine morphological characteristics, physiological and biochemical characteristics, molecular biology and enzy | GB/T 29396-2012: Detection and identification of Burkholderia glumae (Kurita and Tabei) Urakami et al.
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Detection and identification of Burkholderia glumae (Kurita
ICS 65.020.01
B16
National Standards of People's Republic of China
Valley rice bacterial blight pathogen identification of quarantine
Issued on. 2012-12-31
2013-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. Exit Inspection and Quarantine of Hunan People's Republic of China, Chinese Academy of Inspection and Quarantine, the People's Republic of China
And country SZCIQ.
The main drafters of this standard. Zhu Jinguo, Mojin, water Zhu Fang, Zhao Wenjun, Lifang Rong, Li Yinong, Peng Zi, Zhong Wenying.
Valley rice bacterial blight pathogen identification of quarantine
1 Scope
This standard specifies the paddy rice seeds and other materials rice bacterial blight pathogen Valley (Burkholderiaglumae) quarantine Kam
Given to plant morphology, physiological and biochemical characteristics, molecular biology and enzyme immunoassay techniques as the basis for a clear field observation, separation
Identification, sample preservation methods.
This standard applies to rice materials and related environments rice bacterial blight pathogen detection valley.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 15569-1995 transporting agricultural plant quarantine rules
International inspection of seed (ISTA)
3 Valley rice bacterial blight pathogen basic information
Chinese name. rice bacterial blight pathogen Valley (husk Burkholderia bacteria; husk Pseudomonas).
Scientific name. Burkholderiaglumae (Kurita
Synonyms. PseudomonasglumaeKurita
Disease English. Bacterialgrainrotofrice.
Spp community Bacteria, Proteobacteria Proteobacteria, beta-Proteobacteria Betaproteobacteria, Burkholderia bacteria Head Burk-
holderiales, Burkholderia bacteria Branch Burkhoideriaceae, Burkholderia Burkholderia.
Seed infection is the main way long-distance transmission.
Valley rice bacterial blight pathogen additional information, see Appendix A.
4 principle of the method
According to the morphological characteristics of rice plants were field observation, and the use of isolation and culture, molecular biology and enzyme-linked immunological screening, physiological
Biochemical identification and pathogenicity determination of rice bacterial blight pathogen Valley plant material were determined.
5 Equipment and Materials
5.1 freezing high-speed centrifuge. speed ≤15000r/min.
5.2 PCR amplification.
5.3 incubator. 28 ℃ ± 1 ℃.
5.4 Microscope. Lens was 10 × ~ 100 ×.
5.5 Balance. Precision 0.001g.
5.6 autoclave.
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