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GB/T 2930.5-2017: Rules of seed testing for forage, turfgrass and other herbaceous plant -- Biochemical test for viability
Status: Valid GB/T 2930.5: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 2930.5-2017 | English | 459 |
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Rules of seed testing for forage, turfgrass and other herbaceous plant -- Biochemical test for viability
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GB/T 2930.5-2017
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| GB/T 2930.5-2001 | English | 599 |
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Rules for forage seed testing -- Biochemical test for viability
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GB/T 2930.5-2001
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Basic data | Standard ID | GB/T 2930.5-2017 (GB/T2930.5-2017) | | Description (Translated English) | Rules of seed testing for forage, turfgrass and other herbaceous plant -- Biochemical test for viability | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B21 | | Classification of International Standard | 65.020.20 | | Word Count Estimation | 23,211 | | Date of Issue | 2017-11-01 | | Date of Implementation | 2018-05-01 | | Older Standard (superseded by this standard) | GB/T 2930.5-2001 | | Regulation (derived from) | National Standard Announcement 2017 No. 29 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 2930.5-2017: Rules of seed testing for forage, turfgrass and other herbaceous plant -- Biochemical test for viability
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Rules of seed testing for forage, turfgrass and other herbaceous plant-Biochemical test for viability
ICS 65.020.20
B21
National Standards of People's Republic of China
Replacing GB/T 2930.5-2001
Grass seed inspection procedures
Biochemical (tetrazolium) determination of viability
Rulesofseedtestingforforage, turfgrassandotherherbaceousplant-
(The International Seized Test Association, International regulations
seedtesting, edition2012, Chapter6.Biochemicaltestforviability, MOD)
Posted.2017-11-01
2018-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
GB/T 2930 "grass seed inspection procedures" is divided into 11 parts.
--- GB/T 2930.1 Grass seed testing procedures sample;
--- GB/T 2930.2 Grass seed testing procedures Clarity analysis;
--- GB/T 2930.3 grass seeds test procedures Determination of other plant seeds;
--- GB/T 2930.4 grass seed testing procedures sprout test;
--- GB/T 2930.5 grass seed testing procedures biochemical (tetrazolium) determination of viability;
--- GB/T 2930.6 grass seed testing procedures health determination;
--- GB/T 2930.7 Grass seed testing procedures Species and species determination;
--- GB/T 2930.8 grass seed testing procedures moisture determination;
--- GB/T 2930.9 grass seed testing procedures weight determination;
--- GB/T 2930.10 grass seeds inspection procedures Determination of coated seeds;
--- GB/T 2930.11 grass seed inspection procedures inspection report.
This section GB/T 2930 Part 5.
This section drafted in accordance with GB/T 1.1-2009 given rules.
This Part replaces GB/T 2930.5-2001 "Grass Seeds Test Procedure biochemical (tetrazolium Determination)." This section and
GB/T 2930.5-2001 compared to editorial changes in addition to the main technical changes are as follows.
--- The standard name from the "forage seed testing procedures vitality of biochemistry (tetrazolium) Determination" was renamed the "grass seed testing procedures
Biochemical (tetrazolium) determination of viability "(see cover,.2001 edition cover);
--- In the "Preface", an increase of drafting rules based on the standard preparation (see the foreword);
--- Removed "ISTA Preface" (see the.2001 edition of the ISTA Preface);
--- In the "range" added "ecological grass, ornamental plants and medicinal plants" (see Chapter 1);
--- In the "Normative references" added "GB/T 2930.1 grass seed testing procedures sample" (see Chapter 2);
--- Removed the "test purpose" (see the.2001 version of Chapter 3);
--- Increasing the determination of "principle" (see Chapter 4);
--- Modify Figure 3 in Figure 3 (see Figure 1,.2001 version of Figure 1);
--- Modify the "Table 1" column 2 header to "genus/species name"; in the table increased 9 genera/plant seeds determination procedures, an increase of
"Note 3" and "Note 4" (see Table 1, Table 1 of the.2001 edition);
--- In the "dyed" added about tetrazole solution in the fungicide or antibiotic content (see 6.3);
--- In the "result calculation and presentation" increased the same laboratory and different laboratories to determine the allowable gap provisions; increase
The tetrazolium determination results of the specific expression (see Chapter 7);
--- Increased determination of the maximum allowable gap between the table "Table 2" "Table 3" and "Table 4" (see Table 2 to Table 4).
This section uses the redrafted law to amend the use of International Seed Testing Association (InternationalSeedestingAssociation,
ISTA) Chapter 6 of the International Seeds Inspection Code, Biochemical (Tetrazolium) Assay for Vitality (2012 Edition).
This section and the ISTA "International Seeds Inspection Regulations Chapter 6. Vitality of biochemical (tetrazolium) Determination" (2012 Edition) compared to
There are more structural adjustments, listed in Appendix A of this section and the ISTA "International Seeds Inspection Regulations Chapter 6. Vitality of vitality
(Tetrazolium) Determination "(2012 edition) chapter control list (see Table A.1).
This section and the ISTA "International Seeds Inspection Regulations Chapter 6. Vitality of biochemical (tetrazolium) Determination" (2012 Edition) compared to deposit
In the case of technical differences, the clauses covered by these differences have been identified by the vertical single line (|) at the margin of the outer margin, Appendix B
In the corresponding technical differences and a list of the reasons (see Table B.1).
This part is proposed by the Ministry of Agriculture of the People's Republic of China.
This part of the animal husbandry by the National Standardization Technical Committee (SAC/TC274) centralized.
This part of the drafting unit.Lanzhou University grassland agroecosystem State Key Laboratory of Ministry of Agriculture grass and turf grass seed quality supervision
Inspection and Testing Center (Lanzhou).
The main drafters of this section. Wang Yanrong, Zeng Yanjun, Liu Zhipeng, Yu Ling, Zhang Jianquan, Hu Xiaowen, Zhang Jiyu, Liu Yajie, Liu Wenxian, paragraph
Ting Yu.
This part replaces the standards previously issued as.
--- GB 2930-1982;
--- GB/T 2930.5-2001.
Grass seed inspection procedures
Biochemical (tetrazolium) determination of viability
1 Scope
This section GB/T 2930 specifies the grass seed biochemical (tetrazolium) determination procedures and methods.
This section applies to grass, turfgrass, forage crops, ecological grass, ornamental grasses and medicinal plants and other seed quality test of viability determination.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 2930.1 grass seed test procedures sample
GB/T 2930.2 grass seed testing procedures clarity analysis
GB/T 2930.4 grass seed testing procedures germination test
GB/T 8170 rounded repair rules and limit values of the representation and judgment
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Seed vitality seedviability
Potential seed germination or embryo has the vitality.
4 principle
The use of 2,3,5-triphenyltetrazolium chloride or triphenyltetrazolium bromide (referred to as tetrazole) in the seed tissue involved in the reduction of living cells,
Hydrogen ions are taken from the dehydrogenase to allow the colorless tetrazole to undergo hydrogenation to produce a red, stable, and non-diffusible triphenylmethylcarbazole in living cells.
According to the color and location of the tetrazolium dye, differentiate the viable or dead parts of the seeds. In addition to all the vibrant red dyed seeds
And all unstained non-viable seeds, some stained seeds appear. Partially stained seeds may appear to differ in degree
Of necrotic tissue. Determining the viability of seeds depends on the location and size of necrotic tissue area, not necessarily the depth of color.
5 appliances and reagents
5.1 appliances
The vitality of the biochemical (tetrazolium) determination of the required equipment included.
a) Electrothermal incubator or incubator;
b) stereo microscope or magnifying glass;
c) Petri dishes, beakers, test tubes, small glass bottles;
d) Scalpels, blades, dissecting needles, etc.
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