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Detection and identification of Verticillium dahliae Kleb.
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GB/T 28084-2011
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PDF similar to GB/T 28084-2011
Basic data | Standard ID | GB/T 28084-2011 (GB/T28084-2011) | | Description (Translated English) | Detection and identification of Verticillium dahliae Kleb. | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 11,149 | | Date of Issue | 2011-12-30 | | Date of Implementation | 2012-06-01 | | Quoted Standard | GB 15569 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 23 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the symptoms of Verticillium field identification, sampling, Verticillium dahliae (Verticilliumdahliae Kleb.) Isolation and culture, detection and identification of other methods. This standard applies to cotton and cottonseed plant tissues V. dahliae quarantine detection and identification. | GB/T 28084-2011: Detection and identification of Verticillium dahliae Kleb.---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Verticillium dahliae Kleb.
ICS 65.020.01
B16
National Standards of People's Republic of China
Verticillium dahliae detection and identification of quarantine
Issued on. 2011-12-30
2012-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
The main drafters of this standard. WU Li-feng, Han Shiping, Ning Hong, Bai Xiaodong, Liu Hui Chi, Lisu Fang, Xiong Hongli, Zhang Lei.
Verticillium dahliae detection and identification of quarantine
1 Scope
This standard specifies the symptoms of Verticillium field identification, sampling, separating Verticillium dahliae (VerticilliumdahliaeKleb.) The
Culture, detection and identification methods.
This standard applies to quarantine detection and identification of plant tissues and cotton verticillium wilt of cotton and cotton seeds.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 15569 transporting agricultural plant quarantine rules
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Microsclerotia microsclerotium
Verticillium fungus growth process by one or several separated mycelium enlargement, cell wall thickening, formed in all directions to budding meat
Black eye visible mycelium particles.
3.2
Selective medium selectivemedium
When plant pathogen isolation and culture, to promote the formation of colonies of the target, to inhibit the growth of other non-target organisms, added to the medium
The different carbon sources, the medium having a nitrogen source or the ability to select certain inhibitory substances formed.
Principle 4
Verticillium fungus mainly in the cotton plant, cotton seed, soil, disability organizations, manure, etc., after the infected cotton plants exhibited typical
Type symptoms are an important basis for the field diagnosis; the use of selective media, target pathogens isolated from different contaminated materials, morphology
Characterization.
5 instruments, appliances and reagents
5.1 equipment, appliances
Scales, autoclaves, incubators, biological microscopes, pipettes, forceps, scalpels, vaccination rods, 9cm ~ 12cm petri dishes, beakers,
Slides, cover slips, straw, clean benches and the like.
5.2 Reagents
Unless otherwise indicated, the use of only standard analytical reagent.
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