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GB/T 28081-2011: Detection and identification of tobacco ringspot virus
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| GB/T 28081-2011 | English | 399 |
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Detection and identification of tobacco ringspot virus
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GB/T 28081-2011
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Basic data | Standard ID | GB/T 28081-2011 (GB/T28081-2011) | | Description (Translated English) | Detection and identification of tobacco ringspot virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 17,140 | | Date of Issue | 2011-12-30 | | Date of Implementation | 2012-06-01 | | Quoted Standard | SN/T 1146 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 23 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the tobacco ringspot virus quarantine and identification of the basic principles and methods. This standard applies to tobacco ringspot virus may carry the seeds, seedlings, scaly bulbs, tissue culture propagation materials and products such as quarantine and identification. | GB/T 28081-2011: Detection and identification of tobacco ringspot virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of tobacco ringspot virus
ICS 65.020.01
B16
National Standards of People's Republic of China
Tobacco ringspot virus quarantine and identification methods
Issued on. 2011-12-30
2012-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine Shanghai People's Republic of China
Bureau, the Shenzhen People's Republic of China Entry-Exit Inspection and Quarantine of Jiangsu People's Republic of China.
The main drafters of this standard. Chen Qing, Lin Shiming, Yang Cuiyun, Zhang Yi, Chen Yun, Liao Furong, Zheng Yun, Li Bin.
Tobacco ringspot virus quarantine and identification methods
1 Scope
This standard specifies the basic principles and methods of tobacco ringspot virus quarantine identified.
This standard applies to tobacco ringspot virus may carry the seeds, seedlings, scaly bulbs, tissue culture and other propagation materials and products quarantine
Identification.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 1146 Phytosanitary tobacco ringspot virus quarantine and identification methods.
3 Basic information of tobacco ringspot virus
Chinese name. tobacco ringspot virus.
Name. tobaccoringspotvirus.
Abbreviation. TRSV.
Genus cowpea mosaic virus family Comoviridae, nematodes pass polyhedron virus is a virus Nepovirus.
TRSV route of transmission and could be spread by seed, grafting, mechanical inoculation and mediator.
Tobacco ringspot virus For additional information, see Appendix A.
4 principle of the method
Using two anti-immunosorbent assay (DAS-ELISA) sandwich enzyme-linked antigen-antibody reaction based on in vitro reverse transcription and DNA synthesis in vitro
Reverse transcription polymerase chain reaction to technology (RT-PCR) detection and identification.
5 equipment, appliances, and reagents
5.1 Equipment
Washer, ELISA detector, high-speed refrigerated centrifuge, electronic scales (sense of volume 0.001g), clean bench, vortex mixer, PCR
Instrument, real-time PCR instrument, electrophoresis, electrophoresis tank, UV transmission apparatus, juicer, water bath.
5.2 Appliances
Micropipette (0.5μL, 2μL, 10μL, 20μL, 100μL, 200μL, 1000μL), chemical PCR reaction tubes and (or) 96
Photochemical PCR reaction plate, ELISA plate, a mortar.
5.3 Reagents
Double antibody sandwich enzyme-linked immunosorbent assay reagent (see B.1).
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