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GB/T 28071-2011: Detection and identification of cucumber green mottle mosaic virus
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 28071-2011 | English | 349 |
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Detection and identification of cucumber green mottle mosaic virus
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GB/T 28071-2011
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Basic data | Standard ID | GB/T 28071-2011 (GB/T28071-2011) | | Description (Translated English) | Detection and identification of cucumber green mottle mosaic virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 15,167 | | Date of Issue | 2011-12-30 | | Date of Implementation | 2012-06-01 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 23 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the cucumber green mottle mosaic virus quarantine and identification methods. This standard applies may carry cucumber green mottle mosaic virus seeds, seedlings and its products quarantine and identification. | GB/T 28071-2011: Detection and identification of cucumber green mottle mosaic virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of cucumber green mottle mosaic virus
ICS 65.020.01
B16
National Standards of People's Republic of China
Cucumber green mottle mosaic virus quarantine and identification methods
Issued on. 2011-12-30
2012-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine of Guangdong People's Republic of China
Bureau, Chinese Academy of Inspection and Quarantine.
The main drafters of this standard. Chen Qing, Chen Yun, Liao Furong, Lin Shiming, Wu Bingbing, Feng Lixia, Zhang Yongjiang.
Cucumber green mottle mosaic virus quarantine and identification methods
1 Scope
This standard specifies the cucumber green mottle mosaic virus quarantine and identification methods.
This standard applies to quarantine and identification may carry cucumber green mottle mosaic virus seeds, seedlings and its products.
2 cucumber green mottle mosaic virus Basic Information
Chinese name. cucumber green mottle mosaic virus.
Name. cucumbergreenmottlemosaicvirus.
Abbreviation. CGMMV.
Tobacco mosaic virus belongs to the genus Tobamovirus members.
CGMMV can be spread through contact, it can also be spread by seed. Aphids spread mediator melons and other common viruses can not spread
CGMMV. Grafting and other farming operations is one of the main factors of disease epidemics field. Plants Once the disease if no treatment to kill soil
Soil can also be carriers of the virus, infected soil can also become the source of infection of disease occurrence.
For additional information, see Appendix cucumber green mottle mosaic virus A.
3 PRINCIPLE OF THE METHOD
By immunosorbent assay (DAS-ELISA) based on antigen-antibody reaction of the double-antibody sandwich enzyme-linked, in vitro transcription and DNA synthesis in vitro
Reverse transcription polymerase chain reaction to technology (RT-PCR), and detection and identification based on biological characteristics.
4 equipment, appliances, and reagents
4.1 Equipment
Washer, ELISA detector, high-speed refrigerated centrifuge, electronic scales (sense of volume 0.001g), clean bench, vortex mixer, PCR
Instrument, real-time PCR instrument, electrophoresis, electrophoresis tank, UV transmission instrument, isolated greenhouse, juicer, water bath.
4.2 Appliances
Micropipette (0.5μL, 2μL, 10μL, 20μL, 100μL, 200μL, 1000μL), chemical PGR reaction tube and (or) 96
Photochemical PCR reaction plate, ELISA plate, a mortar.
4.3 Reagents
4.3.1 enzyme-linked assay reagent (see Appendix B).
4.3.2 RT-PCR detection reagent (see Appendix C).
4.3.3 real-time fluorescent RT-PCR detection reagent (see Appendix D).
4.3.4 biological assays (see Appendix E).
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