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GB/T 28062-2025: Code of practice for detection of Candidatus Liberibacter asiaticus by quantitative real-time PCR
Status: Valid GB/T 28062: Evolution and historical versions
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| GB/T 28062-2025 | English | 299 |
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Code of practice for detection of Candidatus Liberibacter asiaticus by quantitative real-time PCR
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GB/T 28062-2025
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| GB/T 28062-2011 | English | 489 |
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Detection of Candidatus Liberibacter asiaticus using the real-time fluorescent PCR
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GB/T 28062-2011
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PDF similar to GB/T 28062-2025
Basic data | Standard ID | GB/T 28062-2025 (GB/T28062-2025) | | Description (Translated English) | Code of practice for detection of Candidatus Liberibacter asiaticus by quantitative real-time PCR | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 14,146 | | Date of Issue | 2025-05-30 | | Date of Implementation | 2025-12-01 | | Older Standard (superseded by this standard) | GB/T 28062-2011 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 28062-2025: Code of practice for detection of Candidatus Liberibacter asiaticus by quantitative real-time PCR
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.020.01
CCSB16
National Standard of the People's Republic of China
Replace GB/T 28062-2011
Detection of Citrus Huanglongbing Disease by Real-time Fluorescence Quantitative PCR
Technical regulations
Released on 2025-05-30
2025-12-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
This document replaces GB/T 28062-2011 "Real-time fluorescence PCR detection method for citrus Huanglongbing bacteria" and is compatible with GB/T 28062-2011
Compared with the previous version, in addition to structural adjustments and editorial changes, the main technical changes are as follows.
--- Changed the sampling method for Huanglongbing samples (see 9.1, 8.2 of the.2011 edition);
--- Changed the Ct value of the positive control (see 11.3.2, 10.2.2 of the.2011 edition);
---Change the fluorescent dye detection primers CQULasF04/CQULasR04 of Bacillus asiaticus to more specific primers
GGTAG-3'), and added the fluorescent labeled probe HLBp (sequence. 5'-AGACGGGTGAGTAACGCG-3')
(See 10.1, F.1.1 of the.2011 edition);
---Added real-time fluorescence PCR detection of the target Bacillus asiaticus ribonucleotide reductase β gene (nrdB gene)
(See 11.3.3).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the National Technical Committee on Plant Quarantine Standardization (SAC/TC271).
This document was drafted by. National Agricultural Technology Extension Service Center, South China Agricultural University, and Southwest University Citrus Research Institute.
The main drafters of this document are Feng Xiaodong, Chen Ranran, Deng Xiaoling, Zheng Zheng, Wang Xuefeng and Zhou Changyong.
The previous versions of this document and the documents it replaces are as follows.
---First published in.2011 as GB/T 28062-2011;
---This is the first revision.
Detection of Citrus Huanglongbing Disease by Real-time Fluorescence Quantitative PCR
Technical regulations
1 Scope
The quantitative PCR detection procedure specifies the laboratory setting, sample collection and processing, detection operation method and result judgment, and describes the confirmation method.
This document is applicable to the real-time fluorescence quantitative PCR of Bacillus asiaticus species in host plants and vectors of Huanglongbing (Psyllid citri citri)
Detection and disease identification.
2 Normative references
The contents of the following documents constitute essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB 5040 Quarantine procedures for the origin of citrus seedlings
GB/T 6682 Specifications and test methods for water used in analytical laboratories
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Citrus Huanglongbing
Note. Phloemobacter is a prokaryotic organism, α-Proteobacteria, Rhizobiales, Rhizobiaceae, Phloemobacter
Liberibacter is a Gram-negative bacterium that is difficult to culture. It is divided into three species. Liberibacter asiaticus, which is distributed in Asia, Africa and America.
3.2
Real-time fluorescence quantitative PCR quantitativereal-time PCR
In vitro amplification of trace DNA fragments. During the amplification process, fluorescent substances are released or the fluorescent substances bind to the amplified products.
The fluorescent signal is released after the fusion, and the quantitative analysis of the target DNA in the starting template is achieved by real-time monitoring of the accumulation of fluorescent signal.
3.3
Ct value cyclethresholdvalue
The number of cycles experienced when the fluorescence signal in each reaction tube reaches the set threshold in the real-time fluorescence PCR reaction.
4 Principles of the method
The real-time fluorescence quantitative PCR detection used in this document includes two detection methods. fluorescent dye method and fluorescent probe method.
The method is to design primers using the unique DNA sequence of Huanglongbing fungus, add fluorescent dyes during PCR amplification, and the fluorescent dyes react with the products formed by amplification.
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