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GB/T 28066-2011 English PDF

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GB/T 28066-2011: Detection and identification of Pseudomonas syringae pv. pisi (Sackett) Young et al.
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GB/T 28066-2011English299 Add to Cart 3 days [Need to translate] Detection and identification of Pseudomonas syringae pv. pisi (Sackett) Young et al. Valid GB/T 28066-2011

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Basic data

Standard ID GB/T 28066-2011 (GB/T28066-2011)
Description (Translated English) Detection and identification of Pseudomonas syringae pv. pisi (Sackett) Young et al.
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.01
Word Count Estimation 13,124
Date of Issue 2011-12-30
Date of Implementation 2012-06-01
Regulation (derived from) Announcement of Newly Approved National Standards No. 23 of 2011
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the clove Pseudomonas pea pathotype biology, serology and molecular biology detection and identification methods. This standard applies to plant seeds, seedlings and other plants and their products cloves Pseudomonas pea pathotype detection and identification.

GB/T 28066-2011: Detection and identification of Pseudomonas syringae pv. pisi (Sackett) Young et al.


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Detection and identification of Pseudomonas syringae pv.pisi (Sackett) Young et al. ICS 65.020.01 B16 National Standards of People's Republic of China Pseudomonas syringae peas Pathotype Quarantine and identification methods pv.pisi (Sackett) Youngetal. Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. Xiamen, People's Republic of China Exit Inspection and Quarantine, People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Lin Shiming, Huang Peng Ying, Yi Jianping, Chen Qing, Liao Furong, Wu Yuan, Chen Yun, Wang Hongyi. Pseudomonas syringae peas Pathotype Quarantine and identification methods

1 Scope

This standard specifies the Pseudomonas syringae Detection Methods peas Pathotype biology, serology and molecular biology. This standard applies to plant seeds, seedlings and other plants and their products Pseudomonas syringae peas Pathotype detection and identification.

2 cloves Pseudomonas peas Pathotype basic information

Chinese name. Pseudomonas syringae pathogenic form peas. Chinese alias. peas (bacterial) or fusarium wilt bacterial leaf spot peas, pea vine blight pathogen Pseudomonas or stem blight bacteria and the like. Scientific name. PseudomonassyringaeVanHal, 1902pv.pisi (Sackett, 1916) Young, DyeandWilkie, 1978. Disease English. bactrialblightofpea. Synonyms. Bacteiumpisi (Sackett) Smith, 1920; Chlorobacterpisi (Sackett) Patel Pseudomonaspisi Sackett, 1916; Pseudomonassyringae Van Hal, 1902; Pytomonas (Sackett) Bergey, etal., 1923. Genus Prokaryotae Procaryotes, proteobacteria door Proteobacteria, γ- Proteobacteria Gang Gammaproteobacteria, Pseudomonas Campestris head Pseudomonadales, Pseudomonas Branch Pseudomonadaceae, Pseudomonas genus Pseudomonas. Pseudomonas syringae additional information pea Pathotype see Appendix A.

3 PRINCIPLE OF THE METHOD

Based on colony morphology of bacteria grown on media, the utilization of carbon sources, based on antigen-antibody reaction of the double-antibody sandwich enzyme-linked immunosorbent Attached assay (DAS-ELISA), in vitro DNA amplification technique based on the polymerase chain reaction (PCR), and wherein the pathogenic bacteria after inoculation And other detection and identification.

4 Reagents

The standard detection methods identified mainly using the following reagents. Magnesium sulfate, dipotassium phosphate, sucrose, glycerin, boric acid, magnesium chloride, peptone, physiological saline, chloroform, isoamyl alcohol, isopropyl alcohol, Tris- Hydrochloric acid, EDTA, SDS (sodium dodecyl sulfate), CTAB (cetyl trimethyl ammonium bromide), ethidium bromide, cephalexin, vancomycin, Cefuroxime acid, bromophenol blue, actinomycetes (bacteria) -one or Nystatin and PCR-related reagents.

5 main instruments

The standard detection methods identified mainly with the following instruments. Clean table, autoclaves, microscopes, incubators, electronic scales, centrifuges, PCR instrument, electrophoresis, gel imaging system, BIOLOG microbial identification system and a microplate reader.

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