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GB/T 27642-2011 English PDF

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GB/T 27642-2011: Bovine individual and parental identification using microsatellite DNA
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Basic data

Standard ID GB/T 27642-2011 (GB/T27642-2011)
Description (Translated English) Bovine individual and parental identification using microsatellite DNA
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B43
Classification of International Standard 65.020.30
Word Count Estimation 17,10
Date of Issue 2011-12-30
Date of Implementation 2012-04-01
Quoted Standard GA/T 383; SN/T 1193
Regulation (derived from) Announcement of Newly Approved National Standards No. 22 of 2011
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the use of microsatellite DNA markers for individual cows and paternity technical regulations. This standard applies to ordinary cow (Bos taurus) individuals and paternity.

GB/T 27642-2011: Bovine individual and parental identification using microsatellite DNA

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Bovine individual and parental identification using microsatellite DNA ICS 65.020.30 B43 National Standards of People's Republic of China Cattle individual and microsatellite DNA Paternity Act Issued on. 2011-12-30 2012-04-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Table of Contents

Introduction Ⅲ 1 Scope 1 2 Normative references 1 3 Terms, Definitions and Abbreviations 1 4 Principle 2 5 anti-pollution measures 2 6 individual identification and paternity test step 2 Appendix A (informative) preparation of reagents and equipment 4 Preparation of Appendix B (Informative Appendix) DNA samples 6 Annex C (informative) Spectral correction and PCR reactions were measured and analyzed 8 Appendix D (Informative Appendix) FAO-ISAG recommendation bovine microsatellite DNA markers 10 Annex E (informative) Calculation method of individual identification and paternity 12

Foreword

Accordance with the standards GB/T 1.1-2009 drafting rules. This standard by the National Standardization Technical Committee on Animal Husbandry (SAC/TC274) centralized. This standard was drafted. National Animal Husbandry. The main drafters of this standard. Liu Chou-sheng, Wang Zhigang, Sun Feizhou, Qiu Xiaotian, Han Xu, in Fuqing, Zhang Guixiang. Cattle individual and microsatellite DNA Paternity Act

1 Scope

This standard specifies the use of microsatellite DNA markers and individual cattle paternity technical regulations. This standard applies to bovine (Bostaurus) individuals and a paternity test.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GA/T 383 forensic DNA laboratory testing specification SN/T 1193 genetic testing laboratory technical requirements 3 Terms, definitions and abbreviations The following terms, definitions and abbreviations apply to this document. 3.1 Polymerase chain reaction polymerasechainreaction, PCR A pair of oligonucleotide primers or four deoxyribonucleotides (dNTPs) as raw materials, at a suitable temperature, ionic conditions and heat A DNA polymerase-catalyzed in vitro synthesized DNA fragment specific process. 3.2 Microsatellite DNA microsateliteDNA Also known as short tandem repeats or simple sequence repeat (shorttandemrepeats, STRs; simplesequencerepeats, SSRs), short nucleotide sequence 1bp ~ 6bp as the basic unit of tandem repeats was like scattered in the genome of an organism. 3.3 Probability of paternity exclusion probabilityofpaternityexclusion, PE By one genetic marker testing, paternity dispute can not exclude the probability of his father. 3.4 Cumulative probability of paternity exclusion cumulativeprobabilityofpaternityexclusion, CPE Through multiple genetic markers to detect a genetic marker systems will not negate the biological father of the individual cumulative probability. 3.5 Paternity index paternityindex, PI Dispute father father provided alleles progeny become the father of probability and random sire offspring become father to provide genetic paternity probability ratio. 3.6 Cumulative paternity index cumulativepaternityindex, CPI According to a certain genetic marker system of multiple markers, to calculate the cumulative index of multiple paternity. 3.7 The relative probability of paternity relativechanceofpaternity, RCP Dispute father is the biological father of the relative probability.

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