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GB/T 27538-2011 English PDF

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GB/T 27538-2011: Animal influenza detection -- Method of pyrosequencing for HA and NA in influenza Virus A (H1N1)
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GB/T 27538-2011English299 Add to Cart 3 days [Need to translate] Animal influenza detection -- Method of pyrosequencing for HA and NA in influenza Virus A (H1N1) Valid GB/T 27538-2011

PDF similar to GB/T 27538-2011


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Basic data

Standard ID GB/T 27538-2011 (GB/T27538-2011)
Description (Translated English) Animal influenza detection -- Method of pyrosequencing for HA and NA in influenza Virus A (H1N1)
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 13,111
Date of Issue 2011-11-21
Date of Implementation 2012-03-01
Quoted Standard GB/T 6682; GB/T 18088; GB 19489; NY/T 541; SN/T 1193
Regulation (derived from) Announcement of Newly Approved National Standards No. 18 of 2011
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the pyrosequencing technology for the A H1N1 influenza virus and its variants in Mexico HA and NA nucleic acid sequence detection methods. This standard applies to generic type A H1N1 influenza virus type A (H1N1) virus and mutants Mexico nucleic acid detection.

GB/T 27538-2011: Animal influenza detection -- Method of pyrosequencing for HA and NA in influenza Virus A (H1N1)


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Animal influenza detection. Method of pyrosequencing for HA and NA in influenza Virus A (H1N1) ICS 11.220 B41 National Standards of People's Republic of China Detecting animal influenza type A H1N1 influenza virus Pyrosequencing detection HA, NA's influenzavirusA (H1N1) Issued on. 2011-11-21 2012-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized. This standard was drafted. People's Republic of China Shandong Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine. The main drafters of this standard. Xu Biao, Liang beads, Ling Zong Shuai, Zhang Taixiang, Yue Zhiqin, Gao Hongwei, Sun Tao, Fangshao Qing, Lin Xiangmei, Han Xueqing, Zhu China, Zhang Hexiao single tiger. Detecting animal influenza type A H1N1 influenza virus Pyrosequencing detection HA, NA's

1 Scope

This standard specifies the pyrosequencing technique for Type A H1N1 influenza virus mutants in Mexico and the HA and NA nucleic acid Sequence Detection. This standard applies to nucleic acid detection type A H1N1 influenza virus type A H1N1 influenza and common virus variants Mexico.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 18088 Entry and Exit Animal Quarantine sampling GB 19489 General requirements for laboratory biosafety NY/T 541 animal disease laboratory sampling method SN/T 1193 genetic testing laboratory technical requirements

3 Abbreviations

The following abbreviations apply to this document. ATP. adenosine triphosphate (adenosinetriphosphate) DNA. deoxyribonucleic acid (deoxyribonucleicacid) DEPC. diethyl pyrocarbonate (diethylpyrocarbonate) PBS. phosphate buffered saline (phosphatebelancedsolution) Pyrosequencing. Pyrosequencing RT-PCR. reverse transcription-polymerase chain reaction (reversetranscriptionpolymerasechainreaction) RNA. ribonucleic acid (ribonucleicacid) Taq enzyme. TaqDNA polymerase Principle 4 Pyrosequencing is a single-stranded DNA template was amplified by PCR and sequencing primer hybridization, and DNA polymerase, ATP sulfuric acid enzymes, fluorescent Luciferase, adenosine triphosphate and dual phosphatase substrate (APS), fluorescein incubation, each added four kinds of dNTPs (dATP, dTTP, dCTP, of dGTP), and as the template pair, this end of the primer dNTP form a covalent bond, a group releasing pyrophosphate (PPi); ATP sulfurylase in The case of the presence of a catalytic APS pyrophosphate formed ATP, ATP driven luciferase-mediated oxidation of luciferin-converting phosphor to oxide phosphor Optical element emits visible light signal proportional to the amount of ATP; the optical signal from the CCD detector (CCD) and collected by the software into peaks. Nucleotide number is proportional to the peak height of the response signal in each optical incorporated. ATP and unincorporated dNTP by the adenosine triphosphate diphosphate Enzymatic degradation, quenched optical signal regeneration and reaction system.

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