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GB/T 27532-2024: Diagnostic techniques for canine distemper
Status: Valid GB/T 27532: Evolution and historical versions
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| GB/T 27532-2024 | English | 439 |
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Diagnostic techniques for canine distemper
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GB/T 27532-2024
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| GB/T 27532-2011 | English | 229 |
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Diagnostic techniques for canine distemper
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GB/T 27532-2011
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Basic data | Standard ID | GB/T 27532-2024 (GB/T27532-2024) | | Description (Translated English) | Diagnostic techniques for canine distemper | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 22,270 | | Date of Issue | 2024-11-28 | | Date of Implementation | 2025-06-01 | | Older Standard (superseded by this standard) | GB/T 27532-2011 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 27532-2024: Diagnostic techniques for canine distemper---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 11.220
CCSB41
National Standard of the People's Republic of China
Replace GB/T 27532-2011
Canine distemper diagnostic technology
Released on 2024-11-28
2025-06-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Table of Contents
Preface III
Introduction IV
1 Scope 1
2 Normative references 1
3 Terms and Definitions 1
4 Abbreviations 1
5 Biosafety measures 2
6 Clinical Diagnosis 2
7 Sample collection, processing and storage 3
8 Colloidal gold immunochromatography 4
9 Virus isolation and identification 4
10 Immunoperoxidase monolayer assay (IPMA) 5
11 Immunoenzyme histochemistry test 7
12 RT-PCR 8
13 Fluorescence RT-PCR 10
14 Comprehensive judgment 12
Appendix A (Normative) Preparation of Reagent Solutions 13
Appendix B (Informative) RT-PCR, Fluorescent RT-PCR Primers and Probe Sequences 15
Appendix C (Informative) Electrophoresis of Canine Distemper Virus RT-PCR Reaction Products 16
Appendix D (Informative) Canine distemper virus fluorescence RT-PCR amplification curve Figure 17
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
This document replaces GB/T 27532-2011 "Diagnostic technology for canine distemper". Compared with GB/T 27532-2011, except for the structural adjustment and compilation
In addition to logical changes, the main technical changes are as follows.
--- Added abbreviations (see Chapter 4);
--- Added biosafety measures (see Chapter 5);
--- Added epidemiology (see 6.1);
--- Added colloidal gold immunochromatography (see Chapter 8);
---Added fluorescent RT-PCR (see Chapter 13);
--- Added comprehensive judgment (see Chapter 14).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Technical Committee on Animal Health Standardization (SAC/TC181).
This document was drafted by. Qingdao Agricultural University, Qingdao Haihua Biological Group Co., Ltd., Fengshi (Qingdao) Marine Technology Co., Ltd.,
China Animal Health and Epidemiology Center, Hebei North University, Shandong Academy of Agricultural Sciences, Qingdao Chengyang District Agricultural and Rural Service Center,
Qingdao Batefel Technology Development Co., Ltd., Qingdao Animal Disease Prevention and Control Center, Shanghai Customs Animal and Plant and Food Inspection and Quarantine Technology Center
Center, Chongqing Animal Disease Prevention and Control Center.
The main drafters of this document are. Shan Hu, Zhang Hongliang, Liu Gang, Song Xiaoming, Ma Qingxia, Wang Yuanyuan, Li Yifei, Zhang Ruihua, Huang Bing, Xiao Ying,
Yang Ruimei, Huang Juan, Zhou Fang, Xu Chao, Li Yan, Lin Jiaxu, Li Mingyi, Yu Yongle, Qin Zhihua, Li Jian, Li Guimei, Duan Xiaoxiao, Bing Qizheng, Liu Lirong,
Li Randong, Wang Jianlin, Wen Jianxin, Qin Xiaobing, Ma Jing, and Yang Zhen.
The previous versions of this document and the documents it replaces are as follows.
---First published in.2011 as GB/T 27532-2011;
---This is the first revision.
Introduction
An acute, highly contagious disease of some raccoons and cats. The body temperature is biphasic in the early stage of the disease, followed by bronchitis, calculus,
It is characterized by pneumonia and gastroenteritis. In the later stage, neurological symptoms such as spasms and convulsions can be seen. In some cases, high keratinization of the nose and foot pads may occur.
The mortality rate of infected canines is 30% to 80%, and that of ferrets can reach 100%. my country's Ministry of Agriculture and Rural Affairs has listed it as
Three types of animal diseases.
Canine distemper virus belongs to the Paramyxoviridae family and the Morbillivirus genus. It is a single-stranded RNA
There is currently only one serotype of the virus.
Canine distemper virus has a wide range of hosts, including Canidae (dogs, foxes, raccoon dogs, wolves, etc.), Mustelidae (minks, ferrets, weasels, etc.), some Procyonidae (raccoons, white-nosed bears, etc.),
Animals such as cats (such as raccoons, leopards, and lions) and cats (tigers, leopards, and lions) are susceptible, and young animals are more susceptible than adult animals. The disease is usually more likely to occur 2 to 3 weeks after weaning.
Canine distemper virus can cause multi-system diseases in animals, including digestive, respiratory and nervous systems.
The clinical symptoms caused by different animal immunity are different. The disease is easily associated with upper respiratory tract infection, canine infectious hepatitis, canine coronavirus infection and other diseases.
The disease is confused with each other and needs to be confirmed by combining clinical diagnosis and laboratory tests.
Canine distemper diagnostic technology
1 Scope
This document describes the clinical diagnosis of canine distemper, colloidal gold immunochromatography, virus isolation and identification, immunoperoxidase monolayer test,
Enzyme histochemistry, RT-PCR, fluorescent RT-PCR and other methods.
This document applies to the diagnosis and surveillance of canine distemper.
2 Normative references
The contents of the following documents constitute essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB 19489 General requirements for laboratory biosafety
NY/T 541 Technical Specification for Collection, Storage and Transportation of Veterinary Diagnostic Samples
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Abbreviations
The following abbreviations apply to this document.
BHQ. black hole quencher
CPE. cytopathic effect
Ct. cycle threshold
DAB. diaminobenzidine
FAM.6-carboxyfluorescein
RNA. ribonucleic acid
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