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GB/T 27537-2011: Animal influenza detection -- Protocol of DNA microarray examination for influenza A virus subtypes
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 27537-2011 | English | 329 |
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Animal influenza detection -- Protocol of DNA microarray examination for influenza A virus subtypes
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GB/T 27537-2011
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Basic data | Standard ID | GB/T 27537-2011 (GB/T27537-2011) | | Description (Translated English) | Animal influenza detection -- Protocol of DNA microarray examination for influenza A virus subtypes | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 14,165 | | Date of Issue | 2011-11-21 | | Date of Implementation | 2012-03-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 18 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the type A influenza virus genotyping chip testing procedures. This standard applies to the sample port of all influenza A virus subtypes (H1 ~ H16, N1 ~ N9) screening. | GB/T 27537-2011: Animal influenza detection -- Protocol of DNA microarray examination for influenza A virus subtypes
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Animal influenza detection. Protocol of DNA microarray examination for influenza A virus subtypes
ICS 11.220
B41
National Standards of People's Republic of China
Detecting animal influenza type A influenza virus genotyping
Microarray procedures
Issued on. 2011-11-21
2012-03-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized.
This standard was drafted. Chinese Academy of Inspection and Quarantine, the PLA Military Academy of Medical Sciences, China Institute of Veterinary Drug monitoring
The police.
The main drafters of this standard. Han Xueqing, Liu Bohua, Li, Lin Xiangmei, Wanghui Yu, Liu, Chen Ru, Hou Yihong, Liu Ye Bing Jia Guangle,
Wushao Jiang Liang beads, Qin Zhifeng, Ning Yi Bao, such as thin clear, Wang Yiqin.
Detecting animal influenza type A influenza virus genotyping
Microarray procedures
1 Scope
This standard specifies the type A influenza virus genotyping chip testing procedures.
This standard applies to the sample port of all influenza A virus subtypes (H1 ~ H16, N1 ~ N9) screening.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Abbreviations
The following abbreviations apply to this document.
ctr1, ctr2 and ctr3. QC probe 1, 2 and 3 (control1, control2andcontrol3)
DEPC. diethyl pyrocarbonate (diethylpyrocarbonate)
dNTP. deoxynucleotide triphosphate (deoxy-ribonucleosidetriphosphate)
PBS. phosphate buffered saline (phosphatebuffersolution)
RNA. ribonucleic acid (ribonucleicacid)
RT-PCR. reverse transcription-polymerase chain reaction (reversetranscriptionpolymerasechainreaction)
SDS. sodium dodecyl sulfate (sodiumdodecylsulfate)
SSC. sodium chloride/sodium citrate buffer (sodiumchloride-sodiumcitratebuffer)
TAMRA. carboxy tetramethylrhodamine (carboxytetramethylrhodamine)
4 Reagents and Instruments
4.1 Reagents
4.1.1 test water
Distilled water, according to GB/T 6682, three water, except as otherwise provided, the use of biological and chemical reagents were of analytical grade.
4.1.2 Extraction Kit
Trizol reagents or commercialization of viral RNA extraction reagent.
4.1.3 A influenza virus multiplex RT-PCR asymmetric synthesis and amplification primers and probes
Specific primers and probes (see Appendix A). After the probe and primer synthesis, the use of water-free RNA enzyme (see Appendix B) was dissolved
To 50μmol, -20 ℃ stored for use.
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