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GB/T 27535-2011: The detection method of hemagglutination inhibition antibody against swine influenza
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 27535-2011 | English | 169 |
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The detection method of hemagglutination inhibition antibody against swine influenza
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GB/T 27535-2011
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Basic data | Standard ID | GB/T 27535-2011 (GB/T27535-2011) | | Description (Translated English) | The detection method of hemagglutination inhibition antibody against swine influenza | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 7,766 | | Date of Issue | 2011-11-21 | | Date of Implementation | 2012-03-01 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 18 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the swine influenza virus hemagglutinin (HA) and hemagglutination inhibition (HI) test technical requirements. This standard applies to swine flu serum HI antibody detection. | GB/T 27535-2011: The detection method of hemagglutination inhibition antibody against swine influenza
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The detection method of hemagglutination inhibition antibody against swine influenza
ICS 11.220
B41
National Standards of People's Republic of China
Swine influenza HI antibody detection methods
Issued on. 2011-11-21
2012-03-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized.
This standard was drafted. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences.
The main drafters of this standard. Qiaozhuan Ling, Yanghuan Liang, Chen Yan, Xin Xiaoguang, Chen Hualan.
Swine influenza HI antibody detection methods
1 Scope
This standard specifies the swine influenza virus hemagglutinin (HA) and hemagglutination inhibition (HI) test of the technical requirements.
This standard applies to swine flu HI serum antibody detection.
2 Instrument
Water bath, 4 ℃ refrigerator.
3 test materials
Single-channel and multi-channel micro pipettes, tips.
4 Reagents
4.1 0.01mol/LpH7.2PBS (see Appendix A A.1).
4.2 trypsin solution (see Appendix A A.2).
4.3 0.01mol/LKIO4 solution (see Appendix A in A.3).
4.4 1% glycerol saline (see Appendix A A.4).
4.5 Aschoff (Alsevers) solution (see Appendix A, A.5).
4.6 0.5% chicken erythrocytes (see Appendix A, A.6).
4.7 HI test antigen and positive and negative serum (see Appendix A, A.7).
5 hemagglutination (HA) test
5.1 Using a multichannel micropipette 1 to 12 well microtiter plate were added 0.05mLPBS, exchange tips.
5.2 draw 0.05mL antigen, adding the first hole, repeatedly beaten 3-5 times to mix.
5.3 lessons from the first hole antigen was added 0.05mL second hole, mix draw 0.05mL added the third hole, so to carry out dilution.
The first 11 holes from the 11th hole to draw 0.025mL abandoned, for the tip. 12th hole as a red blood cell controls.
Each well was added 5.4 0.05mL0.5% (volume fraction) of chicken erythrocytes (red blood cells after adding the chicken Shake).
5.5 Mix gently and let stand at room temperature after 20min observations (20 ℃ ~ 25 ℃) under (if the ambient temperature is too high, it can be placed 4 ℃ environment).
RBC control wells significantly determination results were button-like.
5.6 Results found. the reaction plate is inclined 45 °, was not observed if the teardrop-shaped red blood cells flowing, the penalty for HA positive; completely hemagglutinin (not flow
Drip) virus dilution highest representative of a hemagglutination units (HAU).
6 hemagglutination inhibition (HI) test
6.1 Procedure
6.1.1 serum samples. a serum sample to be subject to inspection "trypsin - heating - periodate method" treated to remove non-specific serum
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