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GB/T 27529-2011 English PDF

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GB/T 27529-2011: Diagnostic techniques for contagious equine metritis
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Basic data

Standard ID GB/T 27529-2011 (GB/T27529-2011)
Description (Translated English) Diagnostic techniques for contagious equine metritis
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 13,142
Date of Issue 2011-11-21
Date of Implementation 2012-03-01
Quoted Standard GB/T 4789.28-2003; GB/T 6682
Regulation (derived from) Announcement of Newly Approved National Standards No. 18 of 2011
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the horse contagious metritis (contagious equine metritis, CEM) diagnostic techniques. This standard applies to horses contagious metritis diagnosis and quarantine.

GB/T 27529-2011: Diagnostic techniques for contagious equine metritis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic techniques for contagious equine metritis ICS 11.220 B41 National Standards of People's Republic of China Ma contagious metritis diagnosis technology Issued on. 2011-11-21 2012-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The Standard Appendix A, Appendix B, Appendix C is a normative appendix. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized. This standard was drafted. People's Republic of China Shandong Entry-Exit-Entry Inspection and Xinjiang People's Republic of China Quarantine Bureau. The main drafters of this standard. China Zhu Liang beads, Zheng Xiaolong, Xiao Xi Zhi, Deng Mingjun, Xinxue Qian, Tanle Yi, Fang Shaoqing, Pu palace, Yue Zhiqin, into the new season, Wang Qun, Sun Tao, Zhao Ran. Ma contagious metritis diagnosis technology

1 Scope

This standard specifies the horse contagious metritis (contagiousequinemetritis, CEM) diagnostic techniques. This standard applies to horse contagious metritis diagnosis and quarantine.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to reach an agreement on this standard is Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 4789.28-2003 Microbiological examination of food hygiene staining, media and reagents Laboratory use specifications and test methods GB/T 6682 Analysis

3 Clinical examination

3.1 Clinical features Stallions do not show reproductive tract infection Horse Taylor coli any clinical symptoms and signs of infection when the mare vaginitis, cervicitis, early Vulva a large number of inflammatory exudate or gray secretions, exudates or secretions late becoming sticky pus, which contains a lot of Multinucleated cells, mucosal cells and cell debris off disintegration. 3.2 determination 3.1 based on clinical examination can make a preliminary determination. To confirm the need for laboratory tests, bacteriological examination is the most reliable diagnosis of the disease Methods.

4 Pathogen Isolation and Identification

4.1 Material preparation 4.1.1 Equipment 4.1.1.1 micropipette. 4.1.1.2 Carbon dioxide incubator. 4.1.1.3 ordinary refrigerator and deep freezer (4 ℃, -20 ℃). 4.1.1.4 centrifuge tube. 4.1.1.5 ordinary biological microscope. 4.1.1.6 darkfield or phase contrast microscopy. 4.1.1.7 slides. 4.1.1.8 ring vaccination. 4.1.1.9 lens cleaning paper. 4.1.2 laboratory water Laboratory water means a laboratory for dissolution, dilution or preparation of aqueous solution, distilled several times or prepared by ion exchange, in line with GB/T 6682 two water requirements. 4.1.3 Medium 4.1.3.1 Transport medium. See Appendix A. 4.1.3.2 selective agar medium. See Appendix A.

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