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GB/T 26625-2011 English PDF

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GB/T 26625-2011: Inspection of grain and oils -- Determination of soybean isoflavone -- High performance liquid chromatography
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GB/T 26625-2011English229 Add to Cart 3 days [Need to translate] Inspection of grain and oils -- Determination of soybean isoflavone -- High performance liquid chromatography Valid GB/T 26625-2011

PDF similar to GB/T 26625-2011


Standard similar to GB/T 26625-2011

GB/T 22505   GB/T 15682   GB/T 22725   GB/T 26879   GB/T 10361   GB/T 26627.1   

Basic data

Standard ID GB/T 26625-2011 (GB/T26625-2011)
Description (Translated English) Inspection of grain and oils -- Determination of soybean isoflavone -- High performance liquid chromatography
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B20
Classification of International Standard 67.060
Word Count Estimation 10,136
Date of Issue 2011-06-16
Date of Implementation 2011-11-01
Quoted Standard GB/T 6682
Regulation (derived from) Announcement of Newly Approved National Standards No. 9 of 2011
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the HPLC soy isoflavones (daidzein, glycitein, genistin, daidzein, glycitein aglycone, genistein) content in principle, reagents and materials, instruments and equipment, reagents preparation and preservation, the steps, the results calculated with said precision requirements. This standard applies to soybeans, soy milk, tempeh soy isoflavone content. This standard test method detection limit of 2. 5mg/kg.

GB/T 26625-2011: Inspection of grain and oils -- Determination of soybean isoflavone -- High performance liquid chromatography


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Inspection of grain and oils. Determination of soybean isoflavone. High performance liquid chromatography ICS 67.060 B20 National Standards of People's Republic of China Inspection of grain and soy isoflavone content measured High Performance Liquid Chromatography Issued on. 2011-06-16 2011-11-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The Standard Appendix A, Appendix B is an informative annex. The standard proposed by the National Food Authority. This standard by the National Standardization Technical Committee OILS. This standard was drafted. Heilongjiang People's Republic of China Exit Inspection and Quarantine, ninety-Grain and Oil Industry Group Co., Ltd. The main drafters of this standard. Gao Yong, Yang Chang-chih, Li Tiezhu, Zhang Hongxiang, Dengli Yu, Wang Yue, Ding Lisha. Inspection of grain and soy isoflavone content measured High Performance Liquid Chromatography

1 Scope

This standard specifies the determination of soy isoflavones (daidzein, glycitein, genistein, daidzein, glycitein HPLC Aglycone, genistein principle) content, reagents and materials, instruments and equipment, reagent preparation and preservation, the steps, the results of calculation and said fine Density requirements. This standard applies to soybeans, soy milk, tempeh determination of soybean isoflavone content. The detection limit of the standard test method was 2.5mg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Laboratory use specifications and test methods GB/T 6682 Analysis Principle 3 Samples with methanol - aqueous ultrasonic oscillation extraction, the extract by centrifugation, concentration, volume, filtered, measured by high performance liquid chromatography, outside Standard method.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade, water should meet GB/T 6682 stipulated a water. 4.1 acetonitrile. chromatography. 4.2 methanol. 4.3 acetic acid. 4.4 90% methanol solution. Take 900mL methanol, 100mL of water, and mix. 4.5 60% methanol solution. Take 600mL methanol, 400mL of water, and mix. 4.6 10% methanol solution. Take 100mL methanol, 900mL of water, and mix. 4.7 0.1% acetic acid solution. Take 1mL acetic acid, into 1000mL volumetric flask with water to volume. 4.8 0.1% acetic acid in acetonitrile. Take 1mL acetic acid, into 1000mL volumetric flask, and dissolved in acetonitrile to volume. 4.9 Soybean glycosides (daidzin). purity not less than 98%. 4.10 genistein (genistin). purity not less than 99%. 4.11 daidzein (daidzein). purity not less than 98%. 4.12 genistein (genistein). purity not less than 98%. 4.13 glycitein (glycitin). purity not less than 98%. 4.14 glycitein aglycone (glycitein). purity not less than 98%. 4.15 Standard stock solution preparation. 4.15.1 soy isoflavones standard stock solution. namely, accurately weighed amount of daidzein, genistein, daidzein, genistein, soybeans Flavin, glycitein aglycone standards, respectively with 60% methanol (4.5) formulated at a concentration of 1mg/mL standard stock solution. -18 ℃ to avoid

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