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GB/T 26618-2011: Protocol of diagnosis for Perkinsosis
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Protocol of diagnosis for Perkinsosis
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GB/T 26618-2011
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Basic data | Standard ID | GB/T 26618-2011 (GB/T26618-2011) | | Description (Translated English) | Protocol of diagnosis for Perkinsosis | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 12,155 | | Date of Issue | 2011-06-16 | | Date of Implementation | 2011-11-01 | | Quoted Standard | GB/T 6682; GB/T 18088 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 9 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the camp echinococcosis diagnosis piano sample collection, parasite culture and microscopic examination, PCR and PCR detection procedures. This standard applies to clams, oysters and other aquatic animals and their products carry the Marine camp piano insects (Perkinsus marinus), Olsen sent piano insects (Perkinsus olseni) other faction piano insect diagnostics can also be used to send the piano echinococcosis monitoring and epidemiological investigation. | GB/T 26618-2011: Protocol of diagnosis for Perkinsosis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of diagnosis for Perkinsosis
ICS 11.220
B41
National Standards of People's Republic of China
School piano echinococcosis diagnostic procedures
Issued on. 2011-06-16
2011-11-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
Appendix A of this standard is a normative appendix, Appendix B and Appendix C are informative appendices.
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee on Animal Epidemic Prevention.
This standard was drafted. China Inspection and Quarantine Science Research Institute, Fujian Entry-Exit Inspection and Quarantine Huangdao State
Marine Environmental Monitoring Center, Shenzhen Entry-Exit Inspection and Quarantine Shanghai.
The main drafters of this standard. Wushao Jiang, Lin Xiangmei, Liu Zheng Teng, Li Xifeng, Liang Yubo, Liu Orientin, Li, Han Xueqing, Jia Guangle, Meilin.
Introduction
Send piano echinococcosis major parasitic diseases that affect the world's shellfish aquaculture development for the Office International des Epizooties (OIE) must be reported aquatic provisions
One disease. The disease was first reported in 1946, when the cause Louisiana Gulf of Mexico large oyster (Crassostrea
virginica) death. So far, it has been found to send the piano insect found in many shellfish America, Europe, Australia, Asia and Africa continents,
Including oysters, abalone, clams, scallops, pearl oysters, cockles and mussels in vivo, and caused serious harm in many areas, and high mortality
Up to 95%.
In 1997, our country in the scallop, scallop, abalone wrinkles, R.philippinarum vivo detection to send the piano insect. From the United States in 2005
China Guangxi Beihai detected in imported oysters school piano insects. Currently, the school has become a major piano insect pathogens affect shellfish aquaculture development of our country
one. According to the results of the three northern Yellow Sea R.philippinarum pies piano worm infection status of the investigation showed that except for March and June
The infection rates were 95% and 90% than other months the infection rate was 100%.
Currently, the school piano worm infection often rely on OIE recommended diagnostic tissue sectioning, FTM tissue culture and PCR methods. group
Histological methods can be directly observed by parasites or tissue injury was observed under a microscope to monitor the distribution of the infection. FTM culture school piano insect
Dormant spores is to send the piano insect trophozoites were cultured After incubation, body volume increases, cell wall thickening, and not breeding, it is a
Traditional effective quantitative detection method to send the piano insect. Real-time PCR assay shellfish school piano insect method is highly sensitive and specific
Strong, testing can be completed in one day, accurate, fully enclosed advantage reactions.
School piano echinococcosis diagnostic procedures
1 Scope
This standard specifies the sample collection to send the piano echinococcosis diagnosis, microscopy and parasite culture, PCR and fluorescent PCR detection operation
Procedures.
This standard applies to clams, oysters and other aquatic animals and their products carried in maritime school piano insect (Perkinsusmarinus), Olson sent
Diagnosis piano insect (Perkinsusolseni) and other faction piano insects can also be used for monitoring and epidemiological investigation faction piano echinococcosis.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 18088 Entry and Exit Animal Quarantine sampling
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
Qin sent sis Perkinsosis
Parkin sis
Qin sent by the marine worm (P.marinus), Olson sent Qin insects (P.olseni) and other parasitic or free in the connective in the host blood cells
Weave A gills, viscera or mantle epithelium caused serious parasitic diseases of aquatic animals.
NOTE. In addition to P.marinus, P.olseni, pathogens also include P.qugwadi, P.chesapeaki, P.andrewsi and P.mediterraneus.
3.2
Ct values \u200b\u200bcyclethreshold
Fluorescent PCR reaction, the fluorescent signal reaches the threshold set number of cycles experienced.
4 Materials
Unless otherwise specified, the use of chemical reagents were of analytical grade.
Test water required to achieve the GB/T 6682 in a water requirements.
4.1 liquid thioglycolate medium (fluidthioglycolatemedia, FTM). Specific preparation methods in Appendix A.
4.2 Lugol iodine solution (Lugol'siodine). preparation see Appendix A.
4.3 2.5% chloramphenicol. preparation see Appendix A.
4.4 1% Nystatin. preparation see Appendix A.
4.5 phenol - chloroform extraction method required related reagents or other DNA extraction kit, see Appendix A.
4.6 10 × PCRbuffer, 25mmol/LMgCl2, dNTP (2.5mmol/L, or 10mmol/L), 5U/μLrTaqDNA poly
Synthase, etc., are commercially available kit components.
4.7 agarose.
4.8 electrophoresis buffer. preparation see Appendix A.
4.9 primers and probes include. PCR primers and fluorescent PCR primers and probes.
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