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GB/T 25220-2010: Inspection of grain and oils -- Determination of ochratoxin A in grains by high performance liquid chromatography and fluorometer
Status: Obsolete
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 25220-2010 | English | 519 |
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Inspection of grain and oils -- Determination of ochratoxin A in grains by high performance liquid chromatography and fluorometer
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GB/T 25220-2010
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Basic data | Standard ID | GB/T 25220-2010 (GB/T25220-2010) | | Description (Translated English) | Inspection of grain and oils -- Determination of ochratoxin A in grains by high performance liquid chromatography and fluorometer | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.040 | | Word Count Estimation | 13,153 | | Date of Issue | 2010-09-26 | | Date of Implementation | 2011-03-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Standard Approval Announcement 2010 No.6 (Total No.161) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the use of high performance liquid chromatography and fluorescence spectrophotometric determination of ochratoxin crude birds observed in A principle, reagents and materials, instruments and equipment, operating procedures and results are shown. This standard applies to wheat, maize, rice in the determination of ochratoxin A in toxins. Immunoaffinity column purification and animal performance liquid chromatography and immunoaffinity column clean fluorescence spectrometry detection limits are 1��g/kg, ion exchange solid phase extraction (SPE) HPLC detection limit of 0. 8��g/kg. | GB/T 25220-2010: Inspection of grain and oils -- Determination of ochratoxin A in grains by high performance liquid chromatography and fluorometer
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Inspection of grain and oils.Determination of ochratoxin A my grains by high performance liquid chromatography and fluorometer
ICS 67.040
X04
National Standards of People's Republic of China
Determination of ochratoxin A in foodstuffs and cereals
High Performance Liquid Chromatography and Fluorescence Spectrophotometry
2010-09-26 release
2011-03-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People 's Republic of China
China National Standardization Management Committee released
Foreword
Appendix A and Appendix B of this standard are informative.
This standard is proposed by the National Grain Bureau.
This standard by the National Grain Standardization Technical Committee.
This standard is responsible for the drafting unit. State Food Bureau Science Research Institute.
This standard participates in the drafting unit. Shanghai National Grain Quality Monitoring Center, Beijing Zhongwei Weikang Technology Co., Ltd., Jilin Province grain and oil hygiene
Inspection station.
The main drafters of this standard. Wang Songxue, Wang Xiong, He Zhijun, Liu entropy, Xue Bin, Zhang Yan, Sun Changpo, Ma Xiaoni, Wang Yan, Gao Xiaochun.
Determination of ochratoxin A in foodstuffs and cereals
High Performance Liquid Chromatography and Fluorescence Spectrophotometry
1 Scope
This standard specifies the use of high performance liquid chromatography and fluorescence spectrophotometric determination of ochratoxin A in food the principles, reagents and materials,
Instruments and equipment, operating procedures and results.
This standard applies to the determination of ochratoxin A in wheat, maize and rice.
Immunoaffinity column purification and high performance liquid chromatography and immunoaffinity column purification fluorescence spectrophotometry detection limit of 1μg/kg, ion exchange
The detection limit of high performance liquid chromatography was 0.8μg/kg.
2 normative reference documents
The terms of the following documents are hereby incorporated by reference into this standard. Whichever is the date of the reference file, which is followed by all
(Excluding corrigenda) or revisions do not apply to this standard, however, encourage the parties to reach an agreement under this standard
Whether you can use the latest version of these files. For undated references, the latest edition applies to this standard.
GB/T 6682 Analytical laboratory water specifications and test methods
3 immunoaffinity column purification by high performance liquid chromatography
3.1 Principles
Ochratoxin A in the sample was extracted with acetonitrile water, and the specific immunological affinity reaction between the antibody and its corresponding antigen was used.
Purification of the extract with an immunoaffinity chromatography column containing ochratoxin A-specific antibody was performed using a high performance liquid chromatograph equipped with a fluorescence detector
Determination of external standard method.
3.2 Reagents and materials
Unless otherwise specified, the reagents used are analytical pure, experimental water should be consistent with GB/T 6682 secondary water requirements.
3.2.1 acetonitrile. pure chromatography.
3.2.2 Methanol. Chromatographic Purification.
3.2.3 Sodium chloride.
3.2.4 glacial acetic acid.
3.2. Tween-20 (Tween-20).
3.2.6 Sodium bicarbonate.
3.2.7 disodium hydrogen phosphate.
3.2.8 potassium dihydrogen phosphate.
3.2.9 Potassium chloride.
3.2.10 concentrated hydrochloric acid.
3.2.11 ochratoxin A (OchratoxinA, OTA) standards. purity ≥ 99%.
3.2.12 Extract. acetonitrile (3.2.1) water = 60 40.
3.2.13 Phosphate buffer solution (PBS). 8.0 g sodium chloride (3.2.3), 1.2 g disodium hydrogen phosphate (3.2.7), 0.2 g potassium dihydrogen phosphate
(3.2.8) and 0.2 g of potassium chloride (3.2.9) were dissolved in about 990 mL of water, adjusted to pH 7.0 with concentrated hydrochloric acid (3.2.10), diluted with water to 1 L.
3.2.14 Litter buffer. 25 g sodium chloride (3.2.3), 5 g sodium bicarbonate (3.2.6) dissolved in water, 0.1 mL Tween-20 (3.2.5)
Diluted with water to 1L.
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