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GB/T 25170-2010: Technical regulation of farm animals genome BAC library construction and preservation
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 25170-2010 | English | 189 |
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Technical regulation of farm animals genome BAC library construction and preservation
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GB/T 25170-2010
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Basic data | Standard ID | GB/T 25170-2010 (GB/T25170-2010) | | Description (Translated English) | Technical regulation of farm animals genome BAC library construction and preservation | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B40 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 8,897 | | Date of Issue | 2010-09-26 | | Date of Implementation | 2011-03-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Standard Approval Announcement 2010 No.6 (Total No.161) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the structure of livestock genomic BAC library grate methods. This standard applies to pigs, cattle, sheep, horses, asses, chicken, ducks, geese and other poultry genomic BAC library construction and preservation. | GB/T 25170-2010: Technical regulation of farm animals genome BAC library construction and preservation
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Technical regulation of farm animals genome BAC library construction and preservation
ICS 65.020.30
B40
National Standards of People's Republic of China
Construction of BAC library of livestock and poultry genome
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2010-09-26 release
2011-03-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People 's Republic of China
China National Standardization Management Committee released
Foreword
This standard is proposed by the Ministry of Agriculture of the People's Republic of China.
This standard is under the jurisdiction of the National Committee for Standardization of Animal Husbandry (SAC/TC274).
This standard is drafted by. Beijing Institute of Animal Husbandry and Veterinary, Chinese Academy of Agricultural Sciences.
The main drafters of this standard. Guan Weijun, Ma Yuehui, Liu Changqing, Pan Chunliu, Liu Hongkun, Fu Yabin, Yu Lulu.
Construction of BAC library of livestock and poultry genome
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1 Scope
This standard specifies the method of constructing BAC library of livestock and poultry genome.
This standard applies to the construction and preservation of the genomic BAC library of pigs, cattle, sheep, horses, donkeys, chickens, ducks and geese.
2 normative reference documents
The terms of the following documents are hereby incorporated by reference into this standard. Whichever is the date of the reference file, which is followed by all
(Excluding corrigenda) or revisions do not apply to this standard, however, encourage the parties to reach an agreement under this standard
Whether you can use the latest version of these files. For undated references, the latest edition applies to this standard.
GB/T 6682 Analytical laboratory water specifications and test methods
3 terms and definitions
The following terms and definitions apply to this standard.
3.1
Genomic library genomelibrary
All fragments of nuclear DNA of a certain biological cell were randomly linked to the vector by recombinant DNA technique and transferred to a suitable
A collection of asexual reproduction of each fragment formed by cell proliferation in the main cell.
3.2
Bacteria Artificial chromosome bacterialartificial engromosome, BAC
Based on Escherichia coli-induced factors, the use of modern recombinant DNA technology in vitro synthetic with high molecular weight to accommodate foreign
The carrier of DNA.
3.3
DNA is linked to DNAligation
In the case of DNA ligase catalysis, the target DNA fragment forms a phosphodiester between the 5 'terminal phosphoric acid adjacent to the vector DNA and the 3' terminal hydroxyl group
Key to the process.
3.4
Competent cells
Changes in cell membrane permeability under specific conditions allow exogenous DNA to enter the recipient cells in a specific state within the cell.
3.5
Electrotransformation
In the role of high voltage pulse, the introduction of exogenous DNA into the process of competent cells.
3.6
Pulse field gel electrophoresis pulsefieldgelelectrophoresis, PFGE
The technique of separating and identifying the biomacromolecule by using the function of the alternating field of the electric field in the pulsed field electrophoresis system.
3.7
Recombinant recombinant
Two or more different DNA fragments are linked to the resulting hybrid DNA molecules.
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