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GB/T 24894-2025: Animal and vegetable fats and oils - Determination of the composition of fatty acids in the 2-position of the triglyceride molecules
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Animal and vegetable fats and oils - Determination of the composition of fatty acids in the 2-position of the triglyceride molecules
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GB/T 24894-2025
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| GB/T 24894-2010 | English | 234 |
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Animal and vegetable fats and oils -- Determination of the composition of fatty acids in the 2-position of the triglyceride molecules
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GB/T 24894-2010
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PDF similar to GB/T 24894-2025
Basic data | Standard ID | GB/T 24894-2025 (GB/T24894-2025) | | Description (Translated English) | Animal and vegetable fats and oils - Determination of the composition of fatty acids in the 2-position of the triglyceride molecules | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X14 | | Classification of International Standard | 67.200.10 | | Word Count Estimation | 10,167 | | Date of Issue | 2025-02-28 | | Date of Implementation | 2025-09-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 24894-2025: Animal and vegetable fats and oils - Determination of the composition of fatty acids in the 2-position of the triglyceride molecules
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GB/T 24894-2025 English version. Animal and vegetable fats and oils - Determination of the composition of fatty acids in the 2-position of the triglyceride molecules
National Standard of the People's Republic of China
ICS 67.200.10CCS X 14
Animal and vegetable oils
Determination of fatty acid composition at 2-position of triglyceride molecule
Animal and vegetable fats and oils-
Determination of the composition of fatty acids in the
2⁃position of the triglyceride molecules
(ISO 6800.1997, MOD)
2025-02-28 Release
2025-09-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Replace GB/T 24894-2010
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
This document replaces GB/T 24894-2010 "Determination of 2-position fatty acid components of triglyceride molecules in animal and vegetable oils and fats" and is compatible with GB/T 24894-
Compared with.2010, in addition to structural adjustments and editorial changes, the main technical changes are as follows.
-- Changed the scope of application of the document (see Chapter 1, Chapter 1 of the.2010 edition);
--Some reagents have been changed (see 5.2, 4.2 of the.2010 edition);
--Some instruments have been changed (see 6.2, 5.2 of the.2010 edition);
--Some operation steps have been changed (see 9.4, 8.4 of the.2010 edition);
-- Changed the experimental data processing and added the calculation formula for the 2-position fatty acid composition (see Chapter 10, Chapter 9 of the.2010 edition);
-- Changed the test report (see Chapter 12, Chapter 11 of the.2010 edition);
-- Deleted the preparation and activity determination of lipase (see Appendix A of the.2010 edition).
This document is modified to adopt ISO 6800.1997 "Determination of the 2-position fatty acid composition of triglyceride molecules in animal and vegetable oils and fats".
Compared with ISO 6800.1997, this document has the following structural adjustments.
-- Added a chapter on “Terms and Definitions”;
--10.1 in ISO 6800.1997 has been deleted to adapt to my country's technical conditions and increase operability.
The technical differences between this document and ISO 6800.1997 and their reasons are as follows.
--Replaced ISO 5508 and ISO 5509 (see 5.4, 6.4, 9.6) with the normative reference GB 5009.168-2016, and
Adapt to the technical conditions of the country;
--ISO 660 (see 9.1) was replaced by the normative reference GB 5009.229-2016 to adapt to my country's technical conditions;
-- Changed the scope (see Chapter 1) to extend the application of the document to all substances with a melting point below 50°C containing four to twenty-two carbon atoms.
Oils and fats containing saturated and unsaturated fatty acids;
--Some reagents have been changed (see 5.2) to apply the alcoholysis of triglycerides to prepare 2-monoglycerides;
--Some instruments have been modified (see 6.2) to apply the alcoholysis of triglycerides to prepare 2-monoglycerides;
--Some operating steps (see 9.4) have been changed to apply the alcoholysis of triglycerides to prepare 2-monoglycerides;
-- Added the calculation formula for the 2-position fatty acid composition in the result presentation (see Chapter 10) to express the calculation method more clearly;
--The test report (see Chapter 12) has been modified to adapt to my country's technical conditions;
-- Deleted Appendix A (normative) Preparation and activity determination of lipase, changed to immobilized lipase and provided enzyme activity
parameter.
The following editorial changes were made to this document.
-- Deleted the inter-laboratory test results (see Annex B of ISO 6800.1997).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed by the State Administration of Grain and Material Reserves.
This document is under the jurisdiction of the National Technical Committee on Standardization of Cereals and Oils (SAC/TC 270).
This document was drafted by. Jiangnan University, National Grain and Material Reserves Administration Scientific Research Institute, Henan University of Technology, Wuhan University of Light Industry, Feng
Yi (Shanghai) Biotechnology R&D Center Co., Ltd.
The main drafters of this document are. Wang Xingguo, Wei Wei, Zhao Chenwei, Jin Qingzhe, Jin Jun, Zeng Wei, Liu Yulan, He Dongping, Xue Yalin, Duan Zhangqun,
Cao Wenming.
The previous versions of this document and the documents it replaces are as follows.
--First published in.2010 as GB/T 24894-2010;
--This is the first revision.
Animal and vegetable oils
Determination of fatty acid composition at 2-position of triglyceride molecule
1 Scope
This document describes a method for the determination of the fatty acid composition of the 2-position (β or endo) of triglyceride molecules in animal and vegetable fats and oils.
This document applies to fats and oils containing saturated and unsaturated fatty acids with 4 to 22 carbon atoms and a melting point below 50 °C.
This document does not apply to greases containing the following components.
-- fatty acids containing fewer than four carbon atoms;
-- fatty acids containing more than 22 carbon atoms;
--Contains hydroxy fatty acids (such as ricinoleic acid).
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB 5009.168-2016 National Food Safety Standard Determination of Fatty Acids in Food
GB 5009.229-2016 National Food Safety Standard Determination of Acid Value in Food
GB/T 5524 Sampling of animal and vegetable fats and oils (GB/T 5524-2008, ISO 5555.2001, IDT)
GB/T 6682 Specifications and test methods for water used in analytical laboratories (GB/T 6682-2008, ISO 3696.1987, IDT)
GB/T 15687 Preparation of animal and vegetable fat samples (GB/T 15687-2008, ISO 661.2003, IDT)
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Principle
After the free fatty acids in the sample were neutralized, they were purified by column chromatography, and the triglycerides were hydrolyzed into 2-monoglycerides by enzymes and separated by thin layer chromatography (TLC).
The fatty acid content was determined by gas chromatography.
5 Reagents
All reagents were of analytical grade. Water was grade II water in accordance with GB/T 6682.
5.1 Reagents for sample cleanup
5.1.1 2-Propanol or ethanol. 95% (volume fraction).
5.1.2 n-Hexane or petroleum ether. boiling range 30 ℃ ~ 60 ℃.
5.1.3 2-Propanol or ethanol. 50% (volume fraction).
5.1.4 Sodium hydroxide solution. 0.5 mol/L.
5.1.5 Phenolphthalein solution. 1 g of phenolphthalein is dissolved in 100 mL of 95% (volume fraction) ethanol.
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