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GB/T 24310-2009 English PDF

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GB/T 24310-2009: Tobacco and tobacco products -- Detecting method of genetically modified organism contents
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Basic data

Standard ID GB/T 24310-2009 (GB/T24310-2009)
Description (Translated English) Tobacco and tobacco products -- Detecting method of genetically modified organism contents
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X85
Classification of International Standard 65.160
Word Count Estimation 22,211
Date of Issue 2009-09-30
Date of Implementation 2009-12-01
Quoted Standard GB/T 5606.1; GB/T 19616
Regulation (derived from) National Standard Approval Announcement 2009 No.10 (Total No.150)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the tobacco seeds, fresh leaves, beginning of the flue-cured tobacco, sampling method and sample room real face detection method to detect genetically modified sheet tobacco and cigarette tobacco proceed. This standard applies to detect genetically modified tobacco and tobacco products.

GB/T 24310-2009: Tobacco and tobacco products -- Detecting method of genetically modified organism contents


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Tobacco and tobacco products.Detecting method of genetically modified organism contents ICS 65.160 X85 National Standards of People's Republic of China GB/T 24310-2009 Tobacco and tobacco products - Transgenic testing methods 2009-09-30 release 2009-12-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of the People 's Republic of China China National Standardization Management Committee released Directory Preface I 1 Scope 1 2 normative reference document 1 3 Terms and definitions 1 4 laboratory requirements 2 5 Reagent 3 Equipment and equipment 7 Sampling 4 DNA extraction method for tobacco leaf samples Qualitative detection of transgenic tobacco 5 Quantitative detection of transgenic tobacco 7 Standard for validation of genetically modified tobacco 12 Test Report 9 Appendix A (normative appendices) treatment methods containing EB electrophoresis solution 10 Appendix B (normative) Various methods of DNA extraction 11 Appendix C (normative) Method for determining the concentration and purity of DNA extracts Appendix D (Normative Appendix) Tobacco Transgenic Detection PCR Amplification System and Amplification Conditions Appendix E (Normative Appendix) Preparation of Solutions 14 Appendix F (normative) Fluorescence Quantitative PCR Amplification System and Amplification Conditions Appendix G (informative) Report on Tobacco Transgenic Test Results GB/T 24310-2009

Foreword

Appendix A, Appendix B, Appendix C, Appendix D, Appendix E, Appendix F are normative appendices, Appendix G is an informative appendix. This standard is proposed by the State Tobacco Monopoly Bureau. This standard is under the jurisdiction of the National Tobacco Standardization Technical Committee (SAC/TC144). The drafting unit of this standard. China Tobacco Import and Export Tobacco Detection Station, the People 's Republic of China Shenzhen Entry - Exit Inspection and Quarantine Bureau. The main drafters of this standard. Guo Zhaokui, Chen Zhannan, Feng Qian, Wan Xiuqing, Yan Peijiang, Li Lijie, Qiao Chan, Sun Hongyu. GB/T 24310-2009 Tobacco and tobacco products - Transgenic testing methods

1 Scope

This standard specifies sample sampling methods for the selection of transgenic components for tobacco seeds, fresh tobacco leaves, flue-cured tobacco, flakes and cigarettes, and Laboratory testing method. This standard applies to the detection of transgenic components of tobacco and tobacco products.

2 normative reference documents

The terms of the following documents are hereby incorporated by reference into this standard. Whichever is the date of the reference file, which is followed by all (Excluding corrigenda) or revisions do not apply to this standard, however, encourage the parties to reach an agreement under this standard Whether you can use the latest version of these files. For undated references, the latest edition applies to this standard. GB/T 5606.1 Cigarettes - Part 1. Sampling GB/T 19616 General principles for bulk sampling of tobacco

3 terms and definitions

The following terms and definitions apply to this standard. 3.1 Genetically engineered tobacco by genetic engineering or modern biotechnology. 3.2 A certain amount of tobacco taken out of a sampling unit, forming a single part. [GB/T 19616-2004, definition 2.6] 3.3 One or more representative sample units selected from a group. 3.4 Samples for laboratory testing or testing, which represent the general sample. [GB/T 19616-2004, definition 2.10] 3.5 The samples of the laboratory were subjected to grinding and homogenization (if required) for analysis and analysis. 3.6 PCR amplification Template DNA sequence In the reaction system containing four kinds of deoxyribonucleic acid (dNTPs), primers, DNA polymerase and the like, Through repeated high temperature denaturation, annealing and extension of the cycle, the final target DNA fragments in geometric multiple amplification. GB/T 24310-2009

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