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GB/T 23406-2009 English PDF

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GB/T 23406-2009: Determination of residues of nitroimidazoles and their metabolites in casing -- LC-MS/MS method
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GB/T 23406-2009English229 Add to Cart 3 days [Need to translate] Determination of residues of nitroimidazoles and their metabolites in casing -- LC-MS/MS method Valid GB/T 23406-2009

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Basic data

Standard ID GB/T 23406-2009 (GB/T23406-2009)
Description (Translated English) Determination of residues of nitroimidazoles and their metabolites in casing -- LC-MS/MS method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B45
Classification of International Standard 67.050
Word Count Estimation 10,166
Date of Issue 2009-03-28
Date of Implementation 2009-07-01
Regulation (derived from) National Standard Approval Announcement 2009 No.4 (Total No.144)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the casings metronidazole (referred to MNZ, the same below) and its metabolite 1-(2-hydroxyethyl) -2-(hydroxymethyl) -5-nitroimidazole (referred MNZOH, the same below), two metronidazole imidazole (referred to as DMZ, the same below) and its metabolites object 2-hydroxymethyl-1-methyl-5-nitroimidazole (referred HMMNI, the same below), isopropyl metronidazole (referred IPZ, the same below), and its metabolites 2-(2-hydroxy propyl) -1-methyl-5-nitroimidazole (referred IPZOH), ronidazole (referred RNZ, the same below) the residual liquid chromatography-mass spectrometry/mass spectrometry Determination. The above nitroimidazoles English name, Structure, CAS number and relative molecular mass See Appendix A. This standard applies to the determination of drugs and their metabolites nitroimidazole residues in casings.

GB/T 23406-2009: Determination of residues of nitroimidazoles and their metabolites in casing -- LC-MS/MS method


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of residues of nitroimidazoles and their metabolites in casing. LC-MS/MS method ICS 67.050 B45 National Standards of People's Republic of China Casings nitroimidazoles and their metabolites Determination of residues of liquid chromatography - mass spectrometry/mass spectrometry Posted 2009-03-28 2009-07-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The Standard Appendix A, Appendix B, Appendix C and Appendix D is an informative annex. This standard is proposed and managed by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China. This standard was drafted. People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Chen Huilan, Ding Tao, Shen Chong-yu, Zhang Xiaoyan, Yang Wen Quan, Zhang Ling, Cai Baoliang, Jiang Yuan, Wang Jin Tao. Casings nitroimidazoles and their metabolites Determination of residues of liquid chromatography - mass spectrometry/mass spectrometry

1 Scope

This standard specifies the casing metronidazole (referred to MNZ, the same below) and its metabolite 1- (2-hydroxyethyl) -2-hydroxymethyl-5-nitroimidazole (Jane Said MNZOH, the same below), dimetridazole (referred to as the DMZ, the same below) and its metabolite 2-hydroxymethyl-1-methyl-5-nitro-imidazole (referred to HMMNI, the same below), isopropyl metronidazole (referred IPZ, hereinafter the same) and its metabolite of 2- (2-hydroxy-propyl) -1-methyl-5-nitroimidazole (abbreviated IPZOH), Ronidazole (referred RNZ, the same below) the residual amount of liquid chromatography - mass spectrometry/mass spectrometry. Said nitroimidazole drugs English name, structural formula, CAS Number and molecular weight Appendix A. This standard applies to the determination of drugs and their metabolites nitroimidazoles residues in casings.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

3 Method summary

After adding the appropriate internal standard samples extracted with ethyl acetate nitroimidazoles and their metabolites, evaporated to dryness and the solution was extracted using liquid chromatography - Tandem mass spectrometer, gradient, internal standard.

4 Reagents and materials

Unless otherwise noted, all reagents were of analytical grade water as a water GB/T 6682 regulations. 4.1 Methanol. HPLC grade. 4.2 ethyl acetate. Ammonium acetate 4.3. HPLC grade. 4.4 isotope internal standard. DMZ-D3, IPZOH-D3, HMMNI-D3, purity ≥98%. 4.5 MNZ, MNZOH, DMZ, HMMNI, IPZ, IPZOH and RNZ standards. purity ≥98%. 4.6 MNZ, MNZOH, DMZ, HMMNI, IPZ, IPZOH and RNZ standard stock solution. 1.0mg/mL. Accurately weigh appropriate Amount of MNZ, MNZOH, DMZ, HMMNI, IPZ, IPZOH and RNZ standard (4.5) with methanol (4.1) dubbed 1.0mg/mL Standard stock solution stock solution stored at 4 ℃ refrigerator for up to 6 months. 4.7 DMZ-D3, IPZOH-D3 and HMMNI-D3 internal standard stock solution. 100μg/mL. Weigh the right amount of DMZ-D3, IPZOH-D3 and HMMNI-D3 internal standard (4.4) with methanol (4.1) dubbed 100μg/mL stock solution, the stock solution stored at 4 ℃ refrigerator. 4.8 DMZ-D3, IPZOH-D3 and HMMNI-D3 internal standard working solution. 1μg/mL. Take appropriate internal standard stock solution (4.7) with methanol (4.1) diluted to 1μg/mL working solution of internal standard working solution was stored at 4 ℃. 4.9 MNZ, MNZOH, DMZ, HMMNI, IPZ, IPZOH and RNZ standard solution. methanol (4.1), respectively, dubbed the concentration In the range of 1ng/mL ~ 50ng/mL standard solution, wherein the concentration of the internal standard solution 50ng/mL, the existing service. 4.10 methanol - water (8 + 2, volume ratio). Measure 20mL methanol (4.1) in 80mL water, and mix.

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