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GB/T 23377-2009 English PDF

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GB/T 23377-2009: Determination of dehydroacetic acid in food -- High performance liquid chromatography
Status: Obsolete
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PDF similar to GB/T 23377-2009


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Basic data

Standard ID GB/T 23377-2009 (GB/T23377-2009)
Description (Translated English) Determination of dehydroacetic acid in food -- High performance liquid chromatography
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 8,875
Date of Issue 2009-04-08
Date of Implementation 2009-05-01
Quoted Standard GB/T 6682
Regulation (derived from) National Standard Approval Announcement 2009 No.3 (Total No.143)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the butter, pickles, fermented soy products, bread, cakes, bakery fillings, complex seasonings, HPLC method for the dehydrogenation of acetic acid content of fruit and vegetable juices. This standard applies to butter, pickles, fermented soy products, bread, cakes, bakery fillings, complex seasonings, determination of acetic acid content of fruit and vegetable juices dehydrogenation. Other food available for implementation. This standard limit of quantification was 5mg/kg.

GB/T 23377-2009: Determination of dehydroacetic acid in food -- High performance liquid chromatography


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Determination of dehydroacetic acid in food. High performance liquid chromatography ICS 67.050 X04 National Standards of People's Republic of China Determination of dehydroacetic acid High Performance Liquid Chromatography Posted 2009-04-08 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. The standard proposed by the Qingdao City of Quality and Technical Supervision. The standard by the China National Institute of Standardization. Drafting of this standard. Qingdao City Product Quality Supervision and Inspection, the National Food Quality Supervision, Inspection and Safety Center. The main drafters of this standard. Yang Hongmei, Zhanghui Zhen, Li Huiying, Wang Xiaobin, Liu Yanqin, Wang Hao, Li Zhaoyong, Tan Yan, Yan Long Bao. Determination of dehydroacetic acid High Performance Liquid Chromatography

1 Scope

This standard specifies the butter, pickles, fermented soy products, bread, pastry, bakery fillings, seasonings, fruit and vegetable juices dehydroacetic acid HPLC method for content. This standard applies to butter, pickles, fermented soy products, bread, pastry, bakery fillings, seasonings, fruit and vegetable juices dehydroacetic acid Determination of other foods may refer to. The standard quantitative limit of 5mg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) Principle 3 Extraction sample dehydrogenation acid with sodium hydroxide solution, degreasing, go after protein processing, high performance liquid chromatography with UV detection assay, External standard.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations. 4.1 Methanol. chromatographically pure. 4.2 n-hexane. 4.3 ammonium acetate. pure class distinctions. 4.4 formic acid solution. 10%. Of 10mL formic acid, add water 90mL, mix. 4.5 ammonium acetate solution. 0.02mol/L. Weigh 1.54g ammonium acetate dissolved in water and dilute to 1L. 4.6 Sodium hydroxide solution. 20g/L. Weigh 20.0g of sodium hydroxide dissolved in water and dilute to 1L. 4.7 Zinc sulfate solution. 120g/L. Weighed 120.0g of zinc sulfate heptahydrate dissolved in water and dilute to 1L. 4.8 methanol solution. 70%. Take 70mL of methanol (4.1), add 30mL water, and mix. 4.9 dehydrogenation acid standard sample. purity ≥98%. 4.10 dehydrogenation acid standard stock solution. 1000mg/L. Accurately weighed 100mg dehydroacetic acid standard samples with hydrogen 10mL20g/L of Sodium hydroxide solution to dissolve, water volume to 100mL, dubbed 1000mg/L of standard stock solution, 4 ℃ save can be used for 3 months. 4.11 dehydrogenation acid working standard solution. Pipette 0.1mL, 1.0mL, 5.0mL, 10.0mL, 20.0mL dehydrogenation acid reserve Solution, dilute with water to 100mL, to give a concentration of 1.0mg/L, 10.0mg/L, 50.0mg/L, 100.0mg/L, 200.0mg/L of Working standard solution, 4 ℃ to save, you can use a month. 5. Apparatus 5.1 High performance liquid chromatograph. equipped with a UV detector or diode array detector.

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