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GB/T 23239-2009: Diagnostic techniques for trypanosomosis evansi
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Diagnostic techniques for trypanosomosis evansi
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GB/T 23239-2009
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Basic data | Standard ID | GB/T 23239-2009 (GB/T23239-2009) | | Description (Translated English) | Diagnostic techniques for trypanosomosis evansi | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 10,126 | | Date of Issue | 2009-03-09 | | Date of Implementation | 2009-05-01 | | Regulation (derived from) | National Standard Approval Announcement 2009 No.2 (Total No.142) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the new blood tablets, thin blood smears stained, capillary set insects, laboratory animals inoculated with pathogen identification methods such as latex agglutination test, indirect hemagglutination test and enzyme-linked immunosorbent assay techniques such as serological tests. This standard applies to diagnose evansi disease, epidemiological investigation, quarantine. | GB/T 23239-2009: Diagnostic techniques for trypanosomosis evansi---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic techniques for trypanosomosis evansi
ICS 11.220
B41
National Standards of People's Republic of China
T.evansi Disease Diagnosis
Posted 2009-03-09
2009-05-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
The standards referring to the World Organisation for Animal Health (OIE) "Terrestrial animal diagnostic tests and vaccines manual written (mammals,
Birds and bees) "(fifth edition, 2004).
This standard Annex A, Annex B and Annex C is informative appendix.
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the national immunization Standardization Technical Committee of animals.
This standard was drafted. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences.
Drafters of this standard. Zhou Jinlin, Shen Jie.
T.evansi Disease Diagnosis
1 Scope
This standard specifies the new blood films, thin blood smear, capillary collection insect, animal vaccinations pathogen identification method and latex agglutination test
Test, indirect hemagglutination test and enzyme-linked immunosorbent assay and other serological tests technology.
This standard applies to diagnostic evansi disease, quarantine, epidemiological investigation.
2 clinical diagnosis
By animal species different, different clinical symptoms. Horses (mules, donkeys) general was acute after, infection, body temperature suddenly increased to 40 ℃
Above, missed a few days, and then through the gap a short time, re-attack the body surface edema is a common symptom, conjunctiva and third eyelid often bleeding spots.
Cattle, buffalo and camels general was chronic, there are occasional intermittent fever, loss of appetite, weight loss, swollen feet and ears, tail dry symptoms, Xu
More chronic infections no obvious lesions.
More than clinical symptoms may be suspected of being infected, diagnosis requires laboratory testing.
3 pathogen identification
3.1 blood samples
Can be taken from the ear vein or tail vein peripheral blood, take the deep part of the blood from the jugular vein or other vein, because T.evansi like parasites in the deep
Part of the blood, when in low parasitaemia, peripheral detection rate is often less than 50% of the deep blood.
3.2 new blood examination
On a clean glass slide drops a small drop of blood, a cover glass, the blood diffusion become cell monolayers, and then an optical microscope (200 times)
Observation activities trypanosomes. To prevent blood dried up when viewed, but also to add a drop of saline or 3% sodium citrate at slides blood
Saline.
3.3 thin blood film staining
Insert a small drop of blood on a clean glass slide at one end of about 20mm at the usual way to push into thin blood film, quickly dried, fixed with methanol
2min, after drying, plus Giemsa staining solution (with France see Appendix A) staining 25min, decanted staining solution, tap water, and dried with
400 times to 1,000 times microscopy.
3.4 Capillary set insect inspection
3.4.1 Material preparation
Heparin-treated capillary tube (inner diameter 1.5mm, length 75mm glass or colorless transparent hard plastic tube, in the proposed blood into a
End suction 3% heparin 10mm, dried and reserve oven 70 ℃ ~ 80 ℃), microscope, one Taiwan, centrifuges, one Taiwan, slides, cover glass
Tablets, 12 or more needles (or metal needles), plasticine (modeling clay).
3.4.2 Acquisition to be tested blood samples
Puncture to obtain blood from the animal ear vein or tail vein. With alcohol cotton wipe intends blood at the skin, dry with a needle puncture blood vessels, blood flow
When the suction capillary up to 40mm deep (ie 70μL blood), the closure is not contaminated with the blood of the other end (available with plasticine or flame heating closed).
3.4.3 Set insects
3.4.4 Checking
In capillary plasma and erythrocyte boundary 1mm at the tube broken (cut, cut), it will be with a small amount of red blood cells in the blood plasma drops
On a glass slide, coverslip, the blood spread into the cell monolayer. Check live trypanosomes at 350 to 400-fold amplification of an optical microscope,
For after further detailed observation of the parasite may be further removed cover glass, dried blood spots, with Giemsa stain for 800 times to 1,500 times put
Observation determine the next big microscope.
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