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GB/T 23187-2008: Determination of lutein in feeds -- High performance liquid chromatography
Status: Valid
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| GB/T 23187-2008 | English | 149 |
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Determination of lutein in feeds -- High performance liquid chromatography
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GB/T 23187-2008
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Basic data | Standard ID | GB/T 23187-2008 (GB/T23187-2008) | | Description (Translated English) | Determination of lutein in feeds -- High performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 5,583 | | Date of Issue | 2008-12-31 | | Date of Implementation | 2009-05-01 | | Quoted Standard | GB/T 6682; GB/T 14699.1; GB/T 20195 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.27 (Total No.140) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the method of high performance liquid chromatography feed lutein. This standard applies to single feed, additive premix feed, concentrated feed and feed with the determination of lutein. Quantitation limit is 0. 5mg/kg. | GB/T 23187-2008: Determination of lutein in feeds -- High performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of lutein in feeds. High performance liquid chromatography
ICS 65.120
B46
National Standards of People's Republic of China
Dietary lutein by HPLC
Posted 2008-12-31
2009-05-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) and focal points.
This standard was drafted. Huazhong Agricultural University, Guangzhou-standard detection technology center, Guangzhou Li Dahl Biotechnology Co., Ltd., Foshan City, South
Biotechnology Co., Ltd. Sea Vader.
The main drafters of this standard. OILS, inflammation in the lake, Chen, Tao Zhengguo, Gao Qin, Kuang-mei, Gu Chui Peng, Huang Kai Bin, Sun Yuguo,
Zhang Antonia.
Dietary lutein by HPLC
1 Scope
This standard specifies the method of high performance liquid chromatography in feed lutein.
This standard applies to single feed, feed additive premix, concentrated feed and feed with the determination of lutein. The limit of quantification
Of 0.5mg/kg.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)
GB/T 14699.1 feed sampling (GB/T 14699.1-2005, ISO 6497.2002, IDT)
GB/T 20195 preparation of animal feed samples (GB/T 20195-2006, ISO 6498.1998, IDT)
Principle 3
Lutein sample after alkaline saponification and solvent extraction, the supernatant was filtered, high performance liquid chromatography separation with purple
External detector and calculate the content of lutein external standard method.
4 Reagents and materials
Unless otherwise specified, the reagents were of analytical grade reagent water in line with GB/T 6682 stipulated a water.
4.1 n-hexane.
4.2 acetone.
4.3 ethanol.
4.4 toluene.
4.5 methanol.
4.6 isopropanol.
4.7 potassium hydroxide.
4.8 over anhydrous sodium sulfate.
4.9 Extraction solvent. n-hexane - acetone - ethanol - toluene (34 + 23 + 20 + 23) mixture.
4.10 methanol solution of potassium hydroxide (400g/L). 40g of potassium hydroxide dissolved in methanol, cooled and diluted to 100mL with methanol.
4.11 sodium sulphate solution (100g/L). 10g of anhydrous sodium sulfate were dissolved in 100mL water.
4.12 Lutein standard stock solution. Weigh accurately lutein standard (content greater than 99.0%) 2mg (accurate to 0.1mg), to join the less
The amount of mobile phase sonication, with the mobile phase constant volume to 100mL brown volumetric flask, as the standard stock solution, 4 ℃ dark preservation, storage period of 3 days.
4.13 Lutein standard working solution. take lutein stock standard solution (4.12) with the mobile phase progressively diluted 0.5μg/mL, 1.0μg/mL,
2.0μg/mL, 5.0μg/mL, 10.0μg/mL, 20.0μg/mL series of standard working solution.
5 Instruments
5.1 constant temperature water bath.
5.2 Analysis of balance. a sense of the amount of 0.0001g.
5.3 High performance liquid chromatograph. with UV-VIS detector.
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