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GB/T 22222-2008 English PDF

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GB/T 22222-2008: Determination of xylitol, sorbitol, maltitol in foods -- High-performance liquid chromatography
Status: Obsolete
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PDF similar to GB/T 22222-2008


Standard similar to GB/T 22222-2008

GB/T 22460   GB/T 22507   GB/T 22509   GB/T 45556   GB/T 15683   

Basic data

Standard ID GB/T 22222-2008 (GB/T22222-2008)
Description (Translated English) Determination of xylitol, sorbitol, maltitol in foods -- High-performance liquid chromatography
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 7,772
Date of Issue 2008-05-16
Date of Implementation 2008-10-01
Quoted Standard GB/T 6682
Regulation (derived from) National Standard Approval Announcement 2008 No.10 (Total No.123)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the foods xylitol, conditions determined by HPLC maltitol and detailed analysis step, sorbitol. This standard applies to chewing gum, cakes, candy, beverages and other foods xylitol, sorbitol, determination maltitol content. This standard xylitol, sorbitol, minimum detection limit of maltitol are 0. 4%.

GB/T 22222-2008: Determination of xylitol, sorbitol, maltitol in foods -- High-performance liquid chromatography


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Determination of xylitol, sorbitol, maltitol in foods. High-performance liquid chromatography ICS 67.050 X04 National Standards of People's Republic of China Food xylitol, sorbitol, Maltitol HPLC Posted 2008-05-16 2008-10-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. The standard proposed by China Institute of Metrology. This standard by the National Standardization Technical Committee on Food industry. This standard was drafted. China Institute of Metrology, Beijing Institute of Nutrition source, Beijing beautiful earth testing center. The main drafters of this standard. Jing, Liu Yufeng, Li Li, Sheng Linghui, Tang Huacheng, Fu Boqiang, Zhaomeng Bin, Shang Yanfen. Food xylitol, sorbitol, Maltitol HPLC

1 Scope

This standard specifies the determination of food xylitol, sorbitol, maltitol HPLC conditions and a detailed analysis of the steps. This standard applies to the determination of xylitol, sorbitol, maltitol content of the chewing gum, cakes, candy, drinks and other foods. The minimum detection limit of this standard xylitol, sorbitol, maltitol limits were 0.4%.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)

3 Method summary

After precipitation samples extracted fat and protein filtration, serum into the high pressure liquid chromatography, column separated by amino, external standard.

4 Reagents and solutions

Unless otherwise indicated, the reagents should be of analytical grade water as a water GB/T 6682 regulations. 4.1 acetonitrile. chromatography. 4.2 zinc acetate solution. Weigh 21.9g zinc acetate, 3mL plus glacial acetic acid dissolved in water and diluted to 100mL. 4.3 potassium ferrocyanide solution. Weigh 10.6g of potassium ferrocyanide, dissolved in water and diluted to 100mL. 4.4 petroleum ether. boiling range 30 ℃ ~ 60 ℃. 4.5 sugar alcohols. Purity ≥99%. 4.6 standard stock solution. Weigh 1g (accurate to 0.1mg) xylitol, sorbitol, maltitol standard, adding about 25g dissolved in water, Weighed (accurate to 0.1mg), a solution of 40mg per gram correspond xylitol, sorbitol, maltitol, 4 ℃ placing a seal can be stored Months. 4.7 standard working solution. Pipette sugar alcohol standard stock solution (4.6) 1mL, 2mL, 3mL, 5mL to 25mL volumetric flask, weighing (fine Indeed to 0.1mg), add water to the mark, weighed (accurate to 0.1mg), respectively, equivalent to 1.60mg/g, 3.20mg/g, 4.80mg/g, 8.00mg/g concentration of the standard. 5. Apparatus Conventional laboratory equipment and the following. 5.1 High performance liquid chromatograph. with a refractive index detector or equivalent detector. 5.2 Column. amino column (4.6mm × 250mm, 5μm) or cation exchange chromatography column (6.5mm × 300mm). 5.3 magnetic stirrer. 5.4 Centrifuge. 4000r/min. Preparation 6 samples 6.1 bulk or particulate samples Take a representative sample of at least 200g, with pulverizer, placed in a sealed container.

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