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US$239.00 · In stock Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 22220-2008: Determination of cholesterol in foods -- High-performance liquid chromatography Status: Obsolete
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 22220-2008 | English | 239 |
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Determination of cholesterol in foods -- High-performance liquid chromatography
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GB/T 22220-2008
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PDF similar to GB/T 22220-2008
Basic data | Standard ID | GB/T 22220-2008 (GB/T22220-2008) | | Description (Translated English) | Determination of cholesterol in foods -- High-performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 6,694 | | Date of Issue | 2008-05-16 | | Date of Implementation | 2008-10-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.10 (Total No.123) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the HPLC conditions cholesterol foods heavy and detailed analysis steps. This standard applies to the determination of the cholesterol content of foods. This standard minimum detection limit of 2. 6 mg/100g. |
GB/T 22220-2008: Determination of cholesterol in foods -- High-performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of cholesterol in foods. High-performance liquid chromatography
ICS 67.050
X04
National Standards of People's Republic of China
Determination of cholesterol in foods
High Performance Liquid Chromatography
Posted 2008-05-16
2008-10-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
The standard proposed by China Institute of Metrology.
This standard by the National Standardization Technical Committee on Food industry.
This standard was drafted. China Institute of Metrology, Beijing Institute of Nutrition source, Beijing beautiful earth testing center.
The main drafters of this standard. Jing, Sheng Linghui, Li Li, Liu Yufeng, Tang Huacheng, Zhaomeng Bin, Shang Yanfen.
Determination of cholesterol in foods
High Performance Liquid Chromatography
1 Scope
This standard specifies the determination of cholesterol in food conditions and detailed analysis step HPLC.
This standard applies to the determination of cholesterol in food.
The standard minimum detection limit of 2.6mg/100g.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)
3 Method summary
The sample was ethanol - potassium hydroxide solution after saponification, petroleum ether and anhydrous ether mixed solution was extracted, dissolved in methanol quantitative, high-efficiency
Liquid chromatograph, external standard.
4 Reagents and solutions
Unless otherwise stated, should be used in the analysis of analytical reagent grade water as a water GB/T 6682 recommended.
4.1 Methanol. chromatographically pure.
4.2 ethanol.
4.3 petroleum ether. boiling range 30 ℃ ~ 60 ℃.
4.4 anhydrous ether.
4.5 over anhydrous sodium sulfate.
4.6 potassium hydroxide.
4.7 60% potassium hydroxide solution. Weigh 60g of potassium hydroxide, 40mL water are mixed and dissolved.
4.8 Cholesterol. Purity ≥99%.
4.9 standard stock solution. Weigh cholesterol standard 0.05g (precise to 0.1mg), dissolve with ethanol volume to 50mL, each solution
Ml equivalent to 1mg cholesterol, seal placed 4 ℃ can be stored for six months.
4.10 standard solution. Pipette stock standard solution (4.9) 1.0mL, 2.0mL, 3.0mL, 5.0mL, with anhydrous ethanol to
10mL, formulated correspond 0.1mg/mL, 0.2mg/mL, 0.3mg/mL, 0.5mg/mL concentration of the standard.
5. Apparatus
Conventional laboratory equipment and the following.
5.1 High performance liquid chromatograph. with a UV detector or equivalent detector.
5.2 Analysis of balance. a sense of the amount of 0.1mg.
5.3 Column. C18 reverse phase column (4.6mm × 150mm, 5μm).
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