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GB/T 21915-2008 English PDF

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GB/T 21915-2008: Determination of natamycin in food -- By high-performance liquid chromatography
Status: Obsolete
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PDF similar to GB/T 21915-2008


Standard similar to GB/T 21915-2008

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Basic data

Standard ID GB/T 21915-2008 (GB/T21915-2008)
Description (Translated English) Determination of natamycin in food -- By high-performance liquid chromatography
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.040
Word Count Estimation 11,120
Date of Issue 2008-05-16
Date of Implementation 2008-11-01
Regulation (derived from) National Standard Approval Announcement 2008 No.9 (Total No.122)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the method of liquid chromatography in food natamycin. This standard applies to the determination of food natamycin. This standard limit of quantification was 0. 5 mg/kg, 0. 5 mg/L.

GB/T 21915-2008: Determination of natamycin in food -- By high-performance liquid chromatography

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of natamycin in food. By high-performance liquid chromatography ICS 67.040 X04 National Standards of People's Republic of China Food natamycin determination of liquid chromatography Posted 2008-05-16 2008-11-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard by the national food safety emergency standardization working group proposed and managed. This standard was drafted. Shenyang Product Quality Supervision and Inspection Centre (Country processed foods and additives Quality Supervision and Inspection Center). The main drafters of this standard. Zhaoli Xiu, Li Naijie, Suxi Hui, Zhang Kai, Zhang Lijun, Lu Cheng-Xue, Yu-Luang, seized Zhao, Huang Shijun, Zhang Fengqing. Food natamycin determination of liquid chromatography

1 Scope

This standard specifies the method of liquid chromatography in food natamycin. This standard applies to the determination of food natamycin. The standard quantitative detection limit of 0.5mg/kg (solid and semi-solid samples), 0.5mg/L (liquid sample). Principle 2 Samples were extracted with methanol, the use of water freezing or centrifugation to remove the fat content in the sample purification way, reversed-phase liquid chromatography - ultraviolet detection Detector measurement, external standard.

3 Reagents and materials

3.1 Water. deionized water or equivalent purity. 3.2 methanol. AR, chromatography. 3.3 Natamycin (natamycin). purity ≥95%. 3.4 standard stock solution. Accurately weigh an appropriate amount of natamycin standard (accurate to 0.1mg), dissolved in methanol, dubbed the concentration of 100μg/mL standard stock solution. 3.5 glacial acetic acid. pure class distinctions. 3.6 0.45μm, 0.22μm syringe filter (aqueous).

4 Instrument

4.1 High performance liquid chromatograph. with UV detector. 4.2 ultrasonic cleaner. 4.3 Electronic balance. a sense of the amount of 0.1mg. 4.4 syringe. one, 10mL. 4.5 funnel. a diameter of about 7cm. 4.6 Refrigerator. -20 ℃ ~ -15 ℃. 4.7 centrifuge.

5 Sample Preparation

5.1 Solid and semi-solid samples 5.1.1 cheese, ham, yogurt sample 10.00g accurately weighed sample was added 30mL of methanol ultrasonic extraction 30min, add water 10mL shake in the refrigerator freezer 1h, frozen samples were taken immediately after the filtration, the filtrate was set to room temperature, followed by over 0.45μm and 0.22μm syringe filter to collect The filtrate was about 2mL machine measurement. Natamycin standard spectra see Figure A. 1, cheese, ham sample recovery diagram see Figure A. 2 and Figure A. 3. 5.1.2 moon cake, pastry samples Accurately weighed 5.00g moon cake skin, placed in a centrifuge tube, add 30mL of methanol ultrasonic extraction 30min, add water 10mL shake Set In centrifuge at 3500r/min centrifugal 5min, the supernatant successively through 0.45μm and 0.22μm syringe filter, collecting the filtrate about 2mL machine measurement. Moon cake, pastries sample recovery diagram see Figure A. 4, Figure A. 5.

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