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GB/T 21772-2008: Chemicals -- Test method of mammalian bone narrow chromosome aberration
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 21772-2008 | English | 209 |
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Chemicals -- Test method of mammalian bone narrow chromosome aberration
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GB/T 21772-2008
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Basic data | Standard ID | GB/T 21772-2008 (GB/T21772-2008) | | Description (Translated English) | Chemicals -- Test method of mammalian bone narrow chromosome aberration | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A80 | | Classification of International Standard | 13.300; 11.100 | | Word Count Estimation | 9,978 | | Date of Issue | 2008-05-12 | | Date of Implementation | 2008-09-01 | | Adopted Standard | OECD No.475-1997, IDT | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 7, 2008 (No. 120 overall) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the chemical mammalian bone marrow chromosome aberration test a range of methods, terminology and definitions, test basic principles, test methods, test data and reports. This standard applies to chemicals mammalian bone marrow chromosome aberration test. | GB/T 21772-2008: Chemicals -- Test method of mammalian bone narrow chromosome aberration---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemicals. Test method of mammalian bone narrow chromosome aberration
ICS 13.300; 11.100
A80
National Standards of People's Republic of China
Chemicals mammalian bone marrow chromosome
Distortion Test Method
Posted 2008-05-12
2008-09-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard is identical with the Organisation for Economic Co-operation and Development (OECD) Chemicals testing guidelines No. 475 (1997), "transfection of mammalian bone marrow
Chromosome aberration test "(in English).
The editorial changes made the following standard.
--- Increasing the scope section;
--- Change the unit of measure of legal units of measurement;
--- Deleted the reference section of the OECD.
This standard is managed by the National Standardization Technical Committee chemicals dangerous (SAC/TC251) and focal points.
This standard is drafted by. China Center for Disease Control and Prevention of Occupational Health and Poison Control.
Participated in the drafting of this standard. Occupational Disease Prevention Hospital in Liaoning Province, Tianjin Inspection and Quarantine Institute of Science and Technology.
The main drafters of this standard. Ai Wei Wu, Qu Bo, Xia Hou powder, Li Xuefei, white feather, Liuqing Jun, Zhang Guoqing, Zhang Yuan.
OECD Introduction
1. This test is used to detect and evaluate the test substance-induced rodent bone marrow chromosomal aberration. Structural chromosome aberrations may
There are two forms, namely, chromosomal aberrations and chromatid aberrations. Increase in polyploidy may indicate that the test substance has led to the number of chromosome aberrations
The potential role. Most chemical mutagen-induced chromosomal aberrations is chromatid aberrations, but chromosome mutation may also occur.
Chromosome mutations and related events are the reason many human genetic diseases and there is ample evidence that, causing oncogenes and tumor suppressor
GM made the chromosomal aberrations and related events, related to human and animal cancer tests.
2. Routine use of this test in rodents. Bone marrow is the target tissue in this test, because it is rich in blood vessels, and a large and easily separated at
Rapid cell processing cycle. Other species and target tissues are not the object of the process.
3. Chromosome aberration test is particularly suitable for those who need to consider the evaluation of in vivo metabolism, pharmacokinetics and DNA- repair process, etc.
Pigment mutagenic hazard, although these factors may differ between different animal species, different organizations. In vivo test in further studies
Vitro detection of mutagenic effects are also useful.
4. If there is evidence that the test substance or its active metabolite do not reach the target tissue, it is not suitable for use in this test.
Chemicals mammalian bone marrow chromosome
Distortion Test Method
1 Scope
This standard specifies the scope of chemicals mammalian bone marrow chromosome aberration test methods, terminology and definitions, the basic principles of the test, the test
Methods, test data and reports.
This standard applies to chemicals mammalian bone marrow chromosome aberration test.
2 Terms and definitions
The following terms and definitions apply to this standard.
2.1
Performance breaks or between chromatid breaks and chromosome structural damage reclosing for single chromatid.
2.2
The performance of two chromatid breaks or breaks at the same site and structural chromosome damage reclosing.
2.3
After the S phase of DNA replication, the nucleus does not enter mitosis, the process starts another S period. The result is chromosomes containing
4,8,16 a chromatid.
2.4
Chromatid smaller than the width of the damage is not colored, and with minimal dislocation chromatids.
2.5
The number of chromosomal changes, different from the normal chromosome number of animals used.
2.6
Haploid chromosomes (shape) multiple of the number, but not including diploid (i.e. like three, four, and so like).
2.7
Observed through the microscope chromosomal changes in cell differentiation medium term, microscope, such as deletions, debris, or within the switch
exchange.
3 test basic principles
Exposure by an appropriate route, to the experimental animals exposed to the test substance, and at the appropriate time after exposure the animals were killed. Prior to sacrifice, animals
Metaphase cells with a blocking agent (eg colchicine or colchicine) process. Then take the bone marrow cells of chromosome preparation, staining and analysis
Metaphase cells chromosomal aberrations.
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