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GB/T 21751-2008: Chemicals -- Test method of mammalian spermatogonial chromosome aberration
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 21751-2008 | English | 174 |
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Chemicals -- Test method of mammalian spermatogonial chromosome aberration
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GB/T 21751-2008
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Basic data | Standard ID | GB/T 21751-2008 (GB/T21751-2008) | | Description (Translated English) | Chemicals -- Test method of mammalian spermatogonial chromosome aberration | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A80 | | Classification of International Standard | 13.300; 11.100 | | Word Count Estimation | 9,925 | | Date of Issue | 2008-05-12 | | Date of Implementation | 2008-09-01 | | Adopted Standard | OECD No.483-1997, IDT | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 7, 2008 (No. 120 overall) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the chemical mammalian spermatogonial chromosome aberration test scope, terms and definitions, test basic principles, test methods, test data and reports. This specification applies to testing chemicals on mammalian spermatogonial cytogenetics toxicity. | GB/T 21751-2008: Chemicals -- Test method of mammalian spermatogonial chromosome aberration---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemicals. Test method of mammalian spermatogonial chromosome aberration
ICS 13.300; 11.100
A80
National Standards of People's Republic of China
Chemicals mammalian spermatogonial chromosome aberration
experiment method
Posted 2008-05-12
2008-09-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard is identical with the Organisation for Economic Co-operation and Development (OECD) Chemicals testing guidelines No. 483 (1997), "mammalian spermatogonia
Cell chromosome aberration test "(in English).
The editorial changes made the following standard.
--- Increasing the scope section;
--- Change the unit of measure of legal units of measurement;
--- Remove the OECD references.
This standard is managed by the National Standardization Technical Committee chemicals dangerous (SAC/TC251) and focal points.
This standard is drafted by. China Center for Disease Control and Prevention of Occupational Health and Poison Control.
Participated in the drafting of this standard. Tianjin Center for Disease Control and Prevention, Guangdong CIQ.
The main drafters of this standard. Ai Wei Wu, a Yang, Liu Keming, Liuqing Jun, Liu Jing, Li Guoxing, Xuchong Hui.
OECD Introduction
1. Objective mammals spermatogonial chromosome aberration test is to identify those which cause mammalian spermatogonial chromosome junction
Constitutive material distortion. Structural aberrations include two types, chromosome aberrations or chromatid aberrations. Most chemical mutagens
The distortion type of chromatid aberrations, but can also occur chromosome aberrations. The method is not intended to detect the number of chromosomal aberrations, or
The number is not used for distortion detection routine. Many human genetic diseases and chromosomal aberrations may be related to changes caused.
2. The test for detection of spermatogonial germ cells chromosome changes, which predict cause heritable mutations in germ cells of possibilities.
3. The test routine use rodents. This in vivo cytogenetic test for the detection of spermatogonial mitotic chromosomes of the abnormal
change. Other target cells are not observed as an object of the present methods.
4. To detect spermatogonia chromatid aberrations after exposure should be checked first cell mitosis, so as not to damage them thereafter
The cell division is lost. When infected spermatogonia become spermatocytes, by division in diakinesis --- stained metaphase Ⅰ phase
Meiotic chromosome aberrations size analysis, we can get more information from infected after spermatogonial stem cells.
5. The trial was designed to study in vivo somatic cell mutagens in the germ cells whether active. In addition, because of spermatogonia
Test takes into account a variety of factors in vivo metabolism, pharmacokinetics and DNA repair processes, etc., it can be used to evaluate the mutagenic risk
Risk sex.
6. The presence of multiple generations testes spermatogonial cells after their exposure to chemicals with a wide range of sensitivity varies. Therefore, the detection of abnormal
Change represents a set of chemical reaction was treated spermatogonial cell populations, most of which are mainly differentiated spermatogonia. Because of the physical and biological
Toledo reasonable Purcell's cell (Sertolicel) barrier and the blood - testis barrier, different generations of spermatogonia in the testis in accordance with its
Location may be exposed or not exposed to the systemic circulation.
7. If there is evidence that the test substance or active metabolite can not reach the target tissue, then this test method is not suitable for the detection of this
substance.
Chemicals mammalian spermatogonial chromosome aberration
experiment method
1 Scope
This standard specifies the scope of chemicals mammalian spermatogonial chromosome aberration test, the terms and definitions, basic principles of the test, the test
Methods, test data and reports.
This specification is applicable to the detection of chemical toxicity to mammalian cytogenetic spermatogonia.
2 Terms and definitions
The following terms and definitions apply to this standard.
2.1
Performance breaks or between chromatid breaks and chromosome structural damage reclosing for single chromatid.
2.2
The performance of two chromatid breaks or breaks at the same site and structural chromosome damage reclosing.
2.3
Chromatid smaller than the width of the damage is not colored, and with minimal dislocation chromatids.
2.4
The number of chromosomal changes, different from the normal chromosome number of animals used.
2.5
Haploid number (shape), but does not include the number (i.e., 3 form, shape, etc. 4) diploid.
2.6
Observed through the microscope chromosomal changes in cell differentiation medium term, microscope, such as deletions, debris, or within the switch
exchange.
3 Test Method
3.1 Test the basic principles
Animal through appropriate means contacting the test sample, the animals were killed after a certain time. Prior to sacrifice, with a metaphase cells blockers
(Eg. colchicine colchicine or amide) treatment. After the germ cells with chromosome specimen preparation and staining, analyzing metaphase cells transfected
Color aberrations.
3.2 Experimental animals and breeding environment
3.2.1 animal species
This test is usually male Chinese hamsters and mice. It is also possible to use other suitable male mammals. Normally you should use a healthy early
In experimental animal strains. At the start of the test, the weight difference between the animal should be as small as possible, does not exceed ± 20% of average weight.
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