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GB/T 21493-2008 English PDF

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GB/T 21493-2008: Determination of phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine of soybean phospholipids
Status: Obsolete
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GB/T 21493-2008English279 Add to Cart 3 days [Need to translate] Determination of phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine of soybean phospholipids Obsolete GB/T 21493-2008

PDF similar to GB/T 21493-2008


Standard similar to GB/T 21493-2008

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Basic data

Standard ID GB/T 21493-2008 (GB/T21493-2008)
Description (Translated English) Determination of phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine of soybean phospholipids
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X14
Classification of International Standard 67.200.20
Word Count Estimation 7,778
Date of Issue 2008-03-06
Date of Implementation 2008-08-01
Regulation (derived from) National Health and Family Planning Commission Notice No. 17 of 2016
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard provides a high-performance liquid chromatography method for simultaneous determination of soybean phospholipids phosphatidylcholine uses, phosphatidylethanolamine, phosphatidylinositol content of the three components of the method. This standard applies to oil soy lecithin, butter oil soybean phospholipids phosphatidylcholine, phosphatidylethanolamine, determination of phosphatidylinositol. This standard does not apply to the determination of soybean and soybean lysophosphatidylcholine lysophosphatidylethanolamine for. This standard phosphatidylcholine detection limit is 1. 21 �� 10-2 mg/mL, phosphatidylethanolamine detection limit is 4. 66 �� 10-2 mg/mL, phosphatidylinositol detection limit of 7. 36 �� 10 -3 mg/mL.

GB/T 21493-2008: Determination of phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine of soybean phospholipids


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Determination of phosphatidylethanolamine, phosphatidylinositol and phosphatidycholine of soybean phospholipids ICS 67.200.20 X14 National Standards of People's Republic of China Soybean phospholipid phosphatidylcholine, Determination of phosphatidylethanolamine, phosphatidylinositol Posted 2008-03-06 2008-08-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard is a reference to the International Association of lecithin phospholipids (ILPS) based on the American Oil Chemists Society (AOCS) and other related methods Analysis on China's national conditions determined. The standard proposed by the National Food Authority. This standard by the National Standardization Technical Committee OILS. This standard was drafted. State Administration of Grain Science Institute. The main drafters of this standard. Wang Yingyao, Ma Rong, Xue Yalin. Soybean phospholipid phosphatidylcholine, Determination of phosphatidylethanolamine, phosphatidylinositol

1 Scope

This standard specifies a High Performance Liquid Chromatography soya lecithin phosphatidylcholine, phosphatidylethanolamine, phosphatidylcholine muscle use The alcohol content of the three components of the method. This standard applies to oil soy lecithin, de-phosphatidylcholine, phosphatidylethanolamine, determination of soybean oil phospholipid phosphatidylinositol. this It does not apply Determination of soybean lysophosphatidylcholine and soy lysophosphatidylethanolamine used. This standard phosphatidylcholine detection limit of 1.21 × 10-2mg/mL, phosphatidylethanolamine detection limit of 4.66 × 10-2mg/mL, Phosphatidylinositol detection limit of 7.36 × 10-3mg/mL. Principle 2 Multiple flow eluted with an equal distribution of the two phases in the column phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol component in solid Phasing different residence time to achieve the purpose of separation. UV detector effluent line detection component, compared with standard series quantitation.

3 Reagents

3.1 hexane. chromatographically pure. 3.2 Isopropanol. chromatography. 3.3 Water. chromatography. 3.4 acetic acid. Chromatographic pure. 3.5 1% acetic acid solution. 1mL glacial acetic acid by chromatography water volume to 100mL. 3.6 phosphatidylcholine. standard substance, the purity of ≥99%. 3.7 phosphatidylethanolamine. standard material, the purity of ≥99%. 3.8 Phosphatidylinositol. standard material, the purity of ≥98%. 3.9 Mobile phase. Vp (n-hexane) + Vp (isopropanol) + Vp (1% acetic acid solution) = 8 + 8 + 1. NOTE. As used phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol standard sources should be consistent with the measured sample, when the sample is measured as soybean lecithin, The three standards should be derived from soybeans.

4 instruments and equipment

4.1 Analytical balance. accuracy of 0.0001g. 4.2 scale straw. 0.5mL, 1mL, 5mL. 4.3 flask. 5mL, 10mL, 100mL. 4.4 cylinder. 100mL, 500mL, 1000mL. 4.5 nitrogen bottle. 4.6 liquid chromatography, into the kind of system, UV detector and a data processor. 4.7 HPLC column. Si-60 column packing particle size of 5μm. Length 250mm, inner diameter of 4.6mm. NOTE. Column length and diameter can be based on the experimental conditions make the appropriate selections. Step 5 Analysis 5.1 liquid balance system Prior to injection analysis, with the mobile phase equilibrium system, control the flow rate of 0.5mL/min, to ensure a smooth baseline, retention time of the sample stability.

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