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US$189.00 · In stock Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 21174-2007: Determination of β-Lactams residues in animal derived food -- Radio-receptor assay method Status: Valid
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| GB/T 21174-2007 | English | 189 |
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Determination of β-Lactams residues in animal derived food -- Radio-receptor assay method
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GB/T 21174-2007
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PDF similar to GB/T 21174-2007
Basic data | Standard ID | GB/T 21174-2007 (GB/T21174-2007) | | Description (Translated English) | Determination of ��-Lactams residues in animal derived food -- Radio-receptor assay method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 7,725 | | Date of Issue | 2007-10-29 | | Date of Implementation | 2008-04-01 | | Regulation (derived from) | China National Standard Approval Announcement2007 No.12 (Total No.112) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the sample preparation and analysis methods Radioreceptor meat and aquatic products in the ��- lactam drug residue determination. This standard applies to meat and fish in the screening assay ��- lactam drug residues. |
GB/T 21174-2007: Determination of β-Lactams residues in animal derived food -- Radio-receptor assay method ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of β-Lactams residues in animal derived food. Radio-receptor assay method
ICS 67.050
X04
National Standards of People's Republic of China
Foods of animal origin β- lactams
Residues of radioreceptor assay
Posted 2007-10-29
2008-04-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Fujian Exit Inspection and Quarantine.
The main drafters of this standard. Zheng Jing, Lin Jie, Huang Xiaorong Yang Fang, Chen Bin, Weng Guozhu, Chen Jian.
This standard is the first release.
Foods of animal origin β- lactams
Residues of radioreceptor assay
1 Scope
This standard specifies the sample preparation and analysis methods radioreceptor Meat and Seafood within β- lactam drug residues determination.
This standard applies to meat and fish in the inner β- lactam drug residue screening assays.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)
3 Sample preparation and preservation
3.1 Sample Preparation
Was removed from the original sample portion representative sample should be as fat removed. The edible part of the organization into a high-speed machines are broken
Quality, thoroughly mixed, and dividing by quartering a sample of not less than 500g. Load a clean container and labeled markers.
Sample number of bacteria in the sample preparation procedure should prevent sample contamination or the occurrence of the change of residue content, for the determination not exceed
106/g.
3.2 Sample preservation
The following sample at -18 ℃ preservation, fresh or frozen tissue samples can be stored 72h at 2 ℃ ~ 6 ℃.
4 Determination
4.1 Principle
Based assay is the continuity of the receptor immunoreactivity in samples of residual β- lactams and [
14C] labeled penicillin G, respectively
Specific receptor binding of microbial cells. The sample was measured by scintillation counting of [. 14C] Content count value (cpm), the count value with a liquid
And sample β- lactams residues in inverse proportion.
4.2 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.
4.2.1 CharmⅡ organization β- lactams assay kit
1).
1) CharmⅡ organization β- lactams assay kit and CharmⅡ liquid scintillation counter is provided by the company's products CharmScience
Trade names. This information is given for the convenience of users of this standard does not imply endorsement of the product. If other equivalent products with the same
Effect, you can use these equivalent products.
4.2.1.1 negative control concentrated powder. Store at 2 ℃ ~ 6 ℃. When dissolved in water by using 10mL to prepare a negative tissue fluid. Negative group
Tissue fluid can be stored at 2 ℃ ~ 6 ℃ in 48h. Such a long time, can be stored frozen at -15 ℃ the following two months, the use of which thawed solution
After freeze solution can be stored at 2 ℃ ~ 6 ℃ 24h.
4.2.1.2 MSU variety of antibiotics Standard. stored at 2 ℃ ~ 6 ℃. Dissolved in 10mL of water when used to prepare a variety of anti-MSU
Standard antibiotic solution (penicillin G wherein the concentration of 1000μg/L). MSU variety of antibiotics preservation method with standard solutions 4.2.1.1.
4.2.1.3 MSU extraction buffer concentrate powder. dissolving when using 1000mL of water, formulated as MSU extraction buffer, in
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