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GB/T 21173-2007: Determination of sulfa drugs residues in animal derived food -- Radio-receptor assay method
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 21173-2007 | English | 189 |
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Determination of sulfa drugs residues in animal derived food -- Radio-receptor assay method
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GB/T 21173-2007
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PDF similar to GB/T 21173-2007
Basic data | Standard ID | GB/T 21173-2007 (GB/T21173-2007) | | Description (Translated English) | Determination of sulfa drugs residues in animal derived food -- Radio-receptor assay method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 7,761 | | Date of Issue | 2007-10-29 | | Date of Implementation | 2008-04-01 | | Regulation (derived from) | China National Standard Approval Announcement2007 No.12 (Total No.112) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the sample preparation and analytical methods for meat and radioreceptor sulfa drug residues in aquatic products measured. This standard applies to meat and fish in the screening assay sulfa drug residues. | GB/T 21173-2007: Determination of sulfa drugs residues in animal derived food -- Radio-receptor assay method
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Determination of sulfa drugs residues in animal derived food. Radio-receptor assay method
ICS 67.050
X04
National Standards of People's Republic of China
Foodstuffs of animal origin sulfa drug residues
Determination radioreceptor assay
Posted 2007-10-29
2008-04-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Fujian Exit Inspection and Quarantine.
The main drafters of this standard. Huang Xiaorong, Challenger, Zheng Jing, Yang Fang, Chen Bin, Chen Jian, Weng Guozhu.
This standard is the first release.
Foodstuffs of animal origin sulfa drug residues
Determination radioreceptor assay
1 Scope
This standard specifies the sample preparation and analysis methods radioreceptor meat and aquatic sulfa drug residues determination.
This standard applies to meat and fish screening assays sulfa drug residue.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)
3 Sample preparation and preservation
3.1 Sample Preparation
Was removed from the original sample portion representative sample should be as fat removed. The edible part of the organization into a high-speed machines are broken
Quality, thoroughly mixed, and dividing by quartering a sample of not less than 500g. Load a clean container and labeled markers.
Sample number of bacteria in the sample preparation procedure should prevent sample contamination or the occurrence of the change of residue content, for the determination not exceed
106/g.
3.2 Sample preservation
The following sample at -18 ℃ preservation, fresh or frozen tissue samples can be stored 72h at 2 ℃ ~ 6 ℃.
4 Determination
4.1 Principle
Based assay is a competitive receptor immunoreactivity, [3H] labeled and sulfamethazine residues in samples of sulfa drugs and microorganisms
Competitive binding was specific receptor on the cell. Was measured by liquid scintillation counting of samples [3H] Content count value (cpm), the count value of
In the sample is inversely proportional to the amount of sulfa drug residues.
4.2 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.
4.2.1 CharmⅡ organization sulfa drugs Assay Kit 1).
1) CharmⅡ organization sulfonamides assay kit and CharmⅡ liquid scintillation counter is provided by the supplier of the company's products CharmScience
Name. This information is given for the convenience of users of this standard does not imply endorsement of the product. If other equivalent products have the same effect
As a result, you can use these equivalent products.
4.2.1.1 negative control concentrated powder. Store at 2 ℃ ~ 6 ℃. When dissolved in water by using 10mL to prepare a negative tissue fluid. Negative group
Tissue fluid can be stored at 2 ℃ ~ 6 ℃ in 48h. Such a long time, can be stored frozen at -15 ℃ the following two months, the use of which thawed solution
After freeze solution can be stored at 2 ℃ ~ 6 ℃ 24h.
4.2.1.2 MSU variety of antibiotics Standard. stored at 2 ℃ ~ 6 ℃. Dissolved in 10mL of water when used to prepare a variety of anti-MSU
Antibiotics standard solution (which sulfamethazine concentration of 1000μg/L). MSU variety of antibiotics standard solution preservation methods
With 4.2.1.1.
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