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GB/T 21165-2007 English PDF

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GB/T 21165-2007: Determination of chloramphenicol residues in casing -- LC-MS/MS method
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PDF similar to GB/T 21165-2007


Standard similar to GB/T 21165-2007

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Basic data

Standard ID GB/T 21165-2007 (GB/T21165-2007)
Description (Translated English) Determination of chloramphenicol residues in casing -- LC-MS/MS method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B45
Classification of International Standard 67.120.99
Word Count Estimation 7,735
Date of Issue 2007-10-31
Date of Implementation 2008-04-01
Quoted Standard GB/T 6682
Regulation (derived from) China National Standard Approval Announcement2007 No.12 (Total No.112)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the casings of chloramphenicol residues in liquid chromatography-tandem mass spectrometry. This standard applies to the determination of chloramphenicol residues in casings.

GB/T 21165-2007: Determination of chloramphenicol residues in casing -- LC-MS/MS method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of chloramphenicol residues in casing. LC-MS/MS method ICS 67.120.99 B45 National Standards of People's Republic of China Determination of chloramphenicol residues in casings Liquid chromatography - tandem mass spectrometry Posted 2007-10-31 2008-04-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard is proposed and managed by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China. This standard was drafted. People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Xu Jinzhong, Wu Bin, Li Li flowers, Shen Chong Yu, Zhao Yun increased, Ding Tao, Chen Huilan, publicity, Jiang Yuan, Cai Baoliang, Tao Wang Jin. Determination of chloramphenicol residues in casings Liquid chromatography - tandem mass spectrometry

1 Scope

This standard specifies the casing of chloramphenicol residues in liquid chromatography - tandem mass spectrometry. This standard applies to the determination of chloramphenicol residues in casings.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987)

3 Method summary

After addition of an internal standard samples extracted with ethyl acetate extract solution was evaporated to dryness, the use of liquid chromatography - tandem mass spectrometer, gradient elution to remove group Mass disturbances, internal standard.

4 Reagents and materials

4.1 Water. Water should be consistent with a GB/T 6682 regulations. 4.2 Methanol. HPLC grade. 4.3 ethyl acetate. AR. Chloramphenicol 4.4 deuterated internal standard. purity ≥99%. Chloramphenicol 4.5 Standard substance. purity ≥99%. 4.6 chloramphenicol standard stock solution. 0.1mg/mL. Accurately weighed amount of chloramphenicol standard substance (4.5), paired with methanol 0.1mg/mL standard stock solution. Stock solution stored at 4 ℃ refrigerator, you can use two months. Chloramphenicol 4.7 deuterated internal standard stock solution. 0.1mg/mL. Accurately weighed amount of deuterated chloramphenicol standard substance (4.4), with methanol A standard stock solution 0.1mg/mL of. Stock solution stored at 4 ℃ refrigerator. Chloramphenicol 4.8 deuterated internal standard working solution. Apply the internal standard stock solution was diluted to 50.0ng/mL working solution, internal standard working solution at 4 ℃ save. 4.9 Chloramphenicol standard solution. blank sample extracts were dubbed chloramphenicol concentration of 0.2ng/mL, 0.5ng/mL, 1.0ng/mL, 2.0ng/mL, 5.0ng/mL, 10.0ng/mL standard solution, the standard solution of internal standard working concentration was 5.0ng/mL. Standard working solution at 4 ℃ to save, use the week.

5 Instruments

5.1 Liquid chromatography - tandem mass spectrometry. equipped with electrospray ionization source. 5.2 Analysis of balance. a sense of the amount of 0.1mg and 0.01g each one. 5.3 rotary evaporator. 5.4 Vortex Mixer. 5.5 centrifuge.

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