|
US$159.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 21033-2007: Determination of immunoglobulin in feeds -- High-performance liquid chromatography
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 21033-2007 | English | 159 |
Add to Cart
|
3 days [Need to translate]
|
Determination of immunoglobulin in feeds -- High-performance liquid chromatography
| Valid |
GB/T 21033-2007
|
PDF similar to GB/T 21033-2007
Basic data | Standard ID | GB/T 21033-2007 (GB/T21033-2007) | | Description (Translated English) | Determination of immunoglobulin in feeds -- High-performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 6,699 | | Date of Issue | 2007-06-21 | | Date of Implementation | 2007-09-01 | | Regulation (derived from) | China National Standard Announcement 2007 No.7 (Total No.107) - National-Standard-Commission | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the method for the determination of IgG levels in feed using high performance liquid chromatography. This standard applies to feed, concentrated feed, feed ingredients containing IgG (including plasma protein powder) Determination of IgG. The minimum detection limit of 5 ��g (sampling 1g, the minimum detectable concentration of 5 mg/kg). | GB/T 21033-2007: Determination of immunoglobulin in feeds -- High-performance liquid chromatography
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of immunoglobulin in feeds. High-performance liquid chromatography
ICS 65.120
B46
National Standards of People's Republic of China
High Performance Liquid Chromatography
Posted 2007-06-21
2007-09-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard by the National Standardization Technical Committee Feed Industry and focal points.
This standard is drafted by. National Feed Quality Supervision and Inspection Center (Wuhan), Wuhan En Peter biotechnology limited liability company.
The main drafters of this standard. Liu Xiaomin, Zhou increase too, He Yifan, Gregory red, Yang Lin.
High Performance Liquid Chromatography
1 Scope
This standard specifies the method for the determination of IgG content in feed by high performance liquid chromatography.
This standard applies to feed, concentrated feed and feed ingredients containing IgG (including plasma protein powder) of immunoglobulin IgG
Determination. The minimum detection limit of 5μg (sample 1g, minimum detectable concentration of 5mg/kg).
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 14699.1 feed samples
GB/T 20195 Animal feed sample preparation
Principle 3
According to the principle of high-performance affinity chromatography, IgG immunoglobulin and ligand connected in phosphate buffer conditions, pH2.5 hydrochloric acid
Elution of IgG glycine conditions.
4 Reagents
Unless otherwise indicated in the analysis using only recognized as analytical reagent, the water is deionized water or equivalent purity, shall comply
GB/T 6682 two water requirements.
4.1 potassium dihydrogen phosphate.
4.2 dipotassium hydrogen.
4.3 Mobile phase A. pH6.5,0.05mol/L phosphate buffer.
4.4 Mobile phase B. pH2.5,0.05mol/L glycine-HCl buffer.
4.5 IgG stock standard solution.
Weigh IgG standard (purity ≥95%) 0.0100g, dissolved and set the volume to 10.0mL, shake, concentration of mobile phase A (4.3)
1.0mg/mL.
4.6 IgG working standard solution.
Take IgG standard stock solution was diluted to a standard series containing IgG0.2,0.4,0.6,0.8,1.0mg/mL with mobile phase A (4.3).
Pro formulated with time.
5. Apparatus
5.1 commonly used laboratory equipment.
5.2 pH meter.
5.3 ultra-pure water equipment.
5.4 homogenizer.
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 21033-2007_English be delivered?Answer: Upon your order, we will start to translate GB/T 21033-2007_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of GB/T 21033-2007_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 21033-2007_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|