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GB/T 19180-2020: Diagnostic techniques for bovine spongiform encephalopathy
Status: Valid GB/T 19180: Evolution and historical versions
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| GB/T 19180-2020 | English | 339 |
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Diagnostic techniques for bovine spongiform encephalopathy
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GB/T 19180-2020
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| GB/T 19180-2003 | English | 279 |
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Diagnostic techniques for bovine spongiform encephalopathy
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GB/T 19180-2003
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PDF similar to GB/T 19180-2020
Basic data | Standard ID | GB/T 19180-2020 (GB/T19180-2020) | | Description (Translated English) | Diagnostic techniques for bovine spongiform encephalopathy | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 18,120 | | Date of Issue | 2020-12-14 | | Date of Implementation | 2020-12-14 | | Older Standard (superseded by this standard) | GB/T 19180-2003 | | Regulation (derived from) | National Standard Announcement No. 28 of 2020 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | | Summary | This standard specifies the technical requirements for clinical diagnosis, laboratory diagnosis and comprehensive determination of BSE diagnosis. This standard applies to the diagnosis of BSE, among which clinical diagnosis is suitable for monitoring and epidemiological investigation of BSE, H��E histopathological staining method is suitable for pathological diagnosis of BSE central nervous system, and immunohistochemical methods and western blotting methods are suitable for BSE The etiological diagnosis. | GB/T 19180-2020: Diagnostic techniques for bovine spongiform encephalopathy---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Diagnosis technology of bovine spongiform encephalopathy)
ICS 11:220
B41
National Standards of People's Republic of China
Replace GB/T 19180-2003
Diagnosis technology of bovine spongiform encephalopathy
2020-12-14 release
2020-12-14 implementation
State Administration for Market Regulation
Issued by the National Standardization Management Committee
Foreword
This standard was drafted in accordance with the rules given in GB/T 1:1-2009:
This standard replaces GB/T 19180-2003 "Diagnostic Technology of Bovine Spongiform Encephalopathy": This standard is compared with GB/T 19180-2003, except
In addition to editorial changes, the main technical changes are as follows:
---Added the scope of application of H·E histopathological staining method, immunohistochemical method and western blotting method, and deleted "also available
Used in the diagnosis of infectious spongiform encephalopathy in other animals" (see Chapter 1);
---Optimized the description of clinical symptoms (see 4:3);
---Optimized the operation steps of H·E histopathological staining method (see 5:1) and immunohistochemistry method (see 5:2);
---Added immunoblotting method (see 5:3):
This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China:
This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181):
This standard was drafted: China Animal Health and Epidemiology Center:
The main drafters of this standard: Wang Zhiliang, Liu Yutian, Sun Chengyou, Chi Tianying, Song Fangfang, Zou Yanli, Yu Xiaojing, Wu Xiaodong, Wang Shushuang:
The previous versions of the standard replaced by this standard are as follows:
---GB/T 19180-2003:
Introduction
Bovine Spongiform Encephalopathy (BSE) is a chronic lethality of cattle caused by prions
Neurological diseases pose great harm to animals and humans: The World Organization for Animal Health (OIE) lists it as an animal that has a serious impact on international trade
One of the diseases, our country classifies it as a kind of animal diseases: The current "Diagnostic Technology of Bovine Spongiform Encephalopathy" (GB/T 19180-2003)
It was promulgated by the former General Administration of Quality Supervision, Inspection and Quarantine on June 4,:2003 and implemented on December 1,:2003: This standard only includes clinical
Three diagnostic methods, including symptoms, histopathology, and immunohistochemistry, do not include another diagnostic method specified by the OIE Terrestrial Animal Manual, namely
Western blot: Western blotting method is more sensitive and specific than immunohistochemical method: Therefore, the immunoblotting method is bovine spongiform brain
A better diagnosis method in disease diagnosis technology, it can better serve the monitoring of mad cow disease in my country:
BSE is divided into typical BSE and atypical BSE: Typical BSE is caused by cattle eating meat and bone meal or feed contaminated with cattle and sheep prions:
Caused, atypical BSE is a sporadic BSE that occurs naturally: Under natural conditions, BSE is transmitted through the digestive tract, and no horizontal and vertical
Evidence of direct transmission: The incubation period of typical BSE is generally 2 to 8 years, with an average of 4 to 5 years: It occurs mostly in cattle aged 4 to 6 years:
It is rare, and it decreases significantly over 6 years old: According to the molecular weight of the pathogen, atypical BSE is divided into H type and L type: Atypical BSE
The average age of onset is 11 years old: The course of BSE usually lasts from several months to a year, and eventually death:
Most BSE cattle have no typical clinical symptoms, so the diagnosis of BSE should be achieved through laboratory testing: The pathogen of BSE is
Isoforms of prion protein in the organism, BSE prion antibodies cannot be detected in sick bovine serum, so serological detection methods are suitable for BSE
It is meaningless: At present, most laboratory diagnostic methods are aimed at detecting the pathogen of BSE, including immunohistochemical methods and western blotting methods:
Methods, ELISA methods, etc:, among which immunohistochemical methods and western blotting methods are BSE diagnostic methods specified in the OIE manual: BSE disease
The bovine central nervous system is damaged by prions, and spongy vacuole degeneration will occur: If the central nervous system is well preserved and autolysis has not occurred, use
H·E histopathological staining method can observe these lesions: The OIE manual stipulates that the H·E histopathological staining method is the diagnosis of BSE
When using immunohistochemical methods to diagnose BSE, the H·E histopathological staining method should be used to assist the diagnosis;
When using Western blotting to diagnose BSE, if the sample is suitable for H·E histopathological staining, then H·E should also be used at the same time:
Histopathological staining method assists the diagnosis:
Diagnosis technology of bovine spongiform encephalopathy
1 Scope
This standard specifies the technical requirements for clinical diagnosis, laboratory diagnosis and comprehensive determination of BSE diagnosis:
This standard is applicable to the diagnosis of BSE, in which clinical diagnosis is applicable to the monitoring and epidemiological investigation of BSE, and H·E histopathological staining
The color method is suitable for the pathological diagnosis of the central nervous system of BSE, and the immunohistochemical method and western blot method are suitable for the etiology of BSE
diagnosis:
2 Abbreviations
The following abbreviations apply to this document:
BSE: Bovine Spongiform Encephalopathy
H·E: Hematoxylinandeosin
HRP: Horseradish peroxidase (Horseradishperoxidase)
PBS: Phosphate buffered solution (Phosphatebufferedsolution)
PK enzyme: Proteinase K (ProteinaseK)
PVDF: Polyvinylidenefluoride
SDS: Sodium dodecylsulfate (Sodiumdodecylsulfate)
TBST: Isotonic buffered salt solution (Tris-HClbuffered solutionwithTween)
3 Clinical diagnosis
3:1 Susceptible animals
Bovines and cats, including all kinds of cattle, all kinds of sheep and all kinds of cats:
3:2 Clinical symptoms
3:2:1 Abnormal behavior
Manifested as restlessness, fear, abnormal shock or depression; involuntary movement, such as teeth grinding, tremor; unwilling to pass through cracked ground or enter animals
Bar etc:
3:2:2 Feeling or reaction allergic
The three senses of sight, hearing and touch are too sensitive: Excessive sensitivity to light and dark changes, external sounds, and neck touch, this is BSE
Characteristic clinical manifestations of sick cattle:
3:2:3 Abnormal movement
The sick cow's gait is "goose-walking", ataxia, extremity extension, sometimes falling to the ground, it is difficult to stand:
3:2:4 Decrease in weight and body condition
The body weight and body condition of the sick cow decreased, showing abnormal weight loss and weak physique:
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