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GB/T 19167-2020: Diagnostic techniques for infectious bursal disease
Status: Valid GB/T 19167: Evolution and historical versions
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| GB/T 19167-2020 | English | 369 |
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Diagnostic techniques for infectious bursal disease
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GB/T 19167-2020
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| GB/T 19167-2003 | English | 279 |
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Diagnostic techniques for infectious bursal disease
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PDF similar to GB/T 19167-2020
Basic data | Standard ID | GB/T 19167-2020 (GB/T19167-2020) | | Description (Translated English) | Diagnostic techniques for infectious bursal disease | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 20,271 | | Date of Issue | 2020-12-14 | | Date of Implementation | 2020-12-14 | | Older Standard (superseded by this standard) | GB/T 19167-2003 | | Quoted Standard | GB 19489 | | Regulation (derived from) | National Standard Announcement No. 28 of 2020 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | | Summary | This standard specifies the technical requirements for clinical diagnosis and laboratory diagnosis of infectious bursal disease. This standard applies to the diagnosis, detection, monitoring and epidemiological investigation of chicken infectious bursal disease. Among them, agar gel immunodiffusion test, reverse transcription polymerase chain reaction (RT-PCR), real-time fluorescent reverse transcription polymerase chain reaction (real-time fluorescent RT-PCR), virus isolation and other diagnostic methods are suitable for clinical suspected samples The detection is separated from the virus, and the agar gel immunodiffusion test method is also suitable for the detection of immune antibody level. | GB/T 19167-2020: Diagnostic techniques for infectious bursal disease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Infectious bursal disease diagnosis technology)
ICS 11:220
B41
National Standards of People's Republic of China
Replace GB/T 19167-2003
Infectious bursal disease diagnosis technology
2020-12-14 release
2020-12-14 implementation
State Administration for Market Regulation
Issued by the National Standardization Management Committee
Foreword
This standard was drafted in accordance with the rules given in GB/T 1:1-2009:
This standard replaces GB/T 19167-2003 "Infectious Bursal Disease Diagnosis Technology": Compared with GB/T 19167-2003, except for editorial amendments
In addition, the main technical changes are as follows:
---Unify "infectious bursal disease" and "infectious bursal disease" as "infectious bursal disease";
---Added normative reference documents (see Chapter 2);
---Added abbreviations (see Chapter 3);
---Added equipment and reagents (see Chapter 5, Chapter 6, Chapter 7, Chapter 8, Chapter 9);
---Added clinical diagnosis (see Chapter 4);
---Added laboratory diagnostic sample collection (see Chapter 5);
--- Deleted the direct immunofluorescence method (see 2:5:2 of the:2003 edition);
--- Deleted the enzyme-linked immunosorbent assay (see Chapter 5 of the:2003 edition);
---Added RT-PCR detection method (see Chapter 7, Appendix C, Appendix D);
---Added real-time fluorescent RT-PCR detection method (see Chapter 8, Appendix E);
--- The detection of serum antibodies has been deleted (see Chapter 4 of the:2003 edition);
---Added cell culture isolation virus detection method (see 9:4:1, Appendix F);
--- Increased the comprehensive judgment of the diagnosis result (see Chapter 10):
Please note that certain contents of this document may involve patents: The issuing agency of this document is not responsible for identifying these patents:
This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China:
This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181):
Drafting organizations of this standard: China Center for Animal Health and Epidemiology, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, China Agricultural Science
College Harbin Veterinary Research Institute:
The main drafters of this standard: Jiang Wenming, Chen Jiming, Liu Yuehuan, Wang Xiaomei, Wang Jingjing, Liu Jue, Gao Yulong, Li Yang, Yu Xiaohui, Liu Shuo,
Zhuang Qingye, Li Jinping, Hou Guangyu, Qi Xiaole, Wang Suchun, Liu Hualei:
The previous versions of the standard replaced by this standard are as follows:
---GB/T 19167-2003:
Introduction
Infectious bursaldisease (IBD), also known as Gumborodisease, is composed of double-stranded RNA
Virus (Birnaviridae) avian double-stranded RNA virus (Avibirnavirus) caused by infectious bursal disease virus (IBDV): Turkey,
Duck, guinea fowl and ostrich can all be infected with IBDV: Chickens aged 3 to 6 weeks have acute and severe disease and have a high mortality rate, while chickens aged 1 to 3 weeks
Often presents subacute or subclinical symptoms: The virus invades the bursa of fabric can cause severe immunosuppression: It is known that IBDV has two serotypes, namely
Serotypes 1 and 2, only type 1 is pathogenic to chickens, turkeys and ducks are subclinical infections, and type 2 is not found to be pathogenic:
This disease has characteristic clinical symptoms and eye pathological changes: A preliminary diagnosis can be made: The diagnosis should be confirmed by pathogen detection:
After:2003, the rapid development of IBD diagnostic technology at home and abroad: Some more convenient and accurate new diagnostic techniques have become IBD diagnosis
An important means of interruption and prevention: The purpose of this revision of IBD diagnostic technology is twofold: First, it is basically consistent with international standards and changes the country:
Domestic standards are significantly lagging behind international standards; the second is to incorporate some new technologies that have been proven to be mature and feasible through years of practice at home and abroad:
In the coming years, we will change the condition that the detection technology in the original standard is less and significantly lags behind the actual application: Revising this standard is conducive to the diagnosis of IBD in my country
And prevention is also conducive to enhancing the authority and practicality of national standards:
This standard refers to the OIE "Terrestrial Animal Diagnostic Test and Vaccine Standard Manual" (2016 Edition), and combines the new research results of related technologies in my country
Make revisions:
Infectious bursal disease diagnosis technology
1 Scope
This standard specifies the technical requirements for clinical diagnosis and laboratory diagnosis of infectious bursal disease:
This standard applies to the diagnosis, detection, monitoring and epidemiological investigation of chicken infectious bursal disease: Agar gel immunodiffusion test
Test, reverse transcription polymerase chain reaction (RT-PCR), real-time fluorescent reverse transcription polymerase chain reaction (real-time fluorescent RT-PCR), virus analysis
The isolation diagnosis method is suitable for the detection of clinical suspected samples and virus separation, and the agar gel immunodiffusion test method is also suitable for immune antibodies
Level detection:
2 Normative references
The following documents are indispensable for the application of this document: For dated reference documents, only the dated version applies to this article
Pieces: For undated references, the latest version (including all amendments) applies to this document:
GB 19489 Laboratory Biosafety General Requirements
3 Abbreviations
The following abbreviations apply to this document:
AGID: Agargel immunodiffusion test (Agargelimmunodiffusiontest)
CEF: Chickembryofibroblastcel
CPE: Cytopathiceffect
IBD: Infectious Bursaldisease
IBDV: Infectious bursaldisease virus
PBS: Phosphate buffered saline (Phosphatebufferedsaline)
PCR: Polymerase chain reaction (Polymerasechain reaction)
RT-PCR: Reverse transcription-polymerase chain reaction (Reversetranscription-polymerasechain reaction)
Real-time RT-PCR: Real-time fluorescent reverse transcription polymerase chain reaction (Real-timereverse transcription-polymerase
chainreaction)
SPF: No specific pathogen (Specificpathogenfree)
TAE: Trihydroxymethylaminomethane-acetic acid-
ethylenediaminetetraaceticacid)
4 Clinical diagnosis
4:1 Epidemiology
4:1:1 Natural infection only occurs in chickens, and all kinds of chickens can be infected:
4:1:2 It mainly occurs in chickens between 2 weeks old and 15 weeks old, chickens between 3 weeks old and 6 weeks old are most susceptible, while chickens between 1 week old and 3 weeks old often show sub-acute
Sexual or subclinical symptoms:
4:1:3 It is often acute and spreads rapidly: It usually starts to die on the third day after infection, reaches a peak at 5 to 7 days, and then drops rapidly:
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