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GB/T 18873-2008: General guide of transmission electron microscope (TEM)-X-ray energy dispersive spectrometry (EDS) quantitative microanalysis for thin biological specimens
Status: Valid GB/T 18873: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 18873-2008 | English | 229 |
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General guide of transmission electron microscope (TEM)-X-ray energy dispersive spectrometry (EDS) quantitative microanalysis for thin biological specimens
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GB/T 18873-2008
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| GB/T 18873-2002 | English | 359 |
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General specification of transmission electron microscope (TEM) -- X-ray energy dispersive spectrum (EDS) quantitative microanalysis for thin biological specimens
| Obsolete |
GB/T 18873-2002
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PDF similar to GB/T 18873-2008
Basic data | Standard ID | GB/T 18873-2008 (GB/T18873-2008) | | Description (Translated English) | General guide of transmission electron microscope (TEM)-X-ray energy dispersive spectrometry (EDS) quantitative microanalysis for thin biological specimens | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | N53 | | Classification of International Standard | 71.040.99 | | Word Count Estimation | 10,165 | | Date of Issue | 2008-06-16 | | Date of Implementation | 2009-03-01 | | Older Standard (superseded by this standard) | GB/T 18873-2002 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 10 of 2008 (total 123) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the transmission electron microscope -X -ray spectrometer quantitative analysis of thin biological specimens technical requirements and specifications. This standard applies to non- ultra thin biological specimen contained elements of a transmission electron microscope X-ray spectroscopy quantitative analysis. | GB/T 18873-2008: General guide of transmission electron microscope (TEM)-X-ray energy dispersive spectrometry (EDS) quantitative microanalysis for thin biological specimens
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
General guide of transmission electron microscope (TEM) -X-ray energy dispersive spectrometry (EDS) quantitative microanalysis for thin biological specimens
ICS 71.040.99
N53
National Standards of People's Republic of China
Replacing GB/T 18873-2002
Thin biological specimens -X-ray transmission electron microscope
EDS quantitative analysis General
Posted 2008-06-16
2009-03-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Table of Contents
Preface Ⅰ
1 Scope 1
2 Terms and definitions 1
3 Fundamentals 1
4 instruments and equipment 1
5 thin biological standards for selection 1
6 2 thin biological specimens
7 Preparation 2
8 Select Instrument Measurement Condition 2
9 Measurement and Analysis Step 3
10 error 3
11 The results of the release 4
Appendix A (informative) Calculation method of biological tissue factor G 5
Reference 6
Foreword
This standard replaces GB/T 18873-2002 "thin biological specimens transmission electron microscope -X-ray spectrum quantitative analysis General."
This standard compared with GB/T 18873-2002 main changes are as follows.
--- Thickness ranging from thin biological specimens set at 50nm ~ 300nm (2002 edition of the 2.1, 2.2; 2.1, 2.2 of this edition);
--- Of common elements when biological testing thin specimens, the recommended value of the acceleration voltage as 35kV ~ 80kV (2002 Edition 7.1.2,
8.1; this edition 7.1.2,8.1);
--- Mechanically propelled microtome into thin slicer (2002 edition 4,5.2; this edition 4,5.2);
--- Do not recommend using the Au (2002 version 5.3,6.2; 5.3,6.2 this edition);
--- Recommended to add liquid nitrogen or frozen samples using electron microscopy station in the cold trap (2002 Version 7.1.6; 7.1.6 edition).
Appendix A of this standard is an informative annex.
This standard by the National Standardization Technical Committee microbeam analysis and focal points.
This standard was drafted. Second Military Medical University, Shanghai Medical College, Fudan University, Shanghai Measurement and Testing Technology
Hospital, Military Medical Academy.
The main drafters of this standard. Yang Yong Ji, Chang Yu, Biao Zhang Xun, Zhang added.
This standard replaces the standards previously issued as follows.
--- GB/T 18873-2002.
Thin biological specimens -X-ray transmission electron microscope
EDS quantitative analysis General
1 Scope
This standard specifies the transmission electron microscope -X-ray spectroscopy quantitative analysis of technical requirements and specifications thin biological specimens.
This standard applies to thin biological specimens contained in a transmission electron microscope, non-quantitative X-ray spectrum of light element analysis.
2 Terms and definitions
The following terms and definitions apply to this standard.
2.1
Means cut using a microtome to a thickness of 50nm ~ 300nm in the biological sample.
2.2
Means cut using a microtome to a thickness of 50nm ~ 300nm biological sample of a standard.
2.3
Biological samples and biological standards of chemical composition of elements factor. Available factor G of formula (1) is calculated.
G = Σ
Where.
3 Fundamentals
With a high energy electron beam irradiation thin biological specimens micro areas of focus in the region contained the elements of atomic excitation by high-energy electron beam
Send produce characteristic X-ray, which corresponds to the characteristic X-ray energy-related elements; integrated intensity of characteristic X-rays corresponding to the concentration of the element.
Spectrometer using a sample of the received characteristic X-ray peak intensity and background intensity (i.e., a continuous X-ray intensity) difference under the same conditions and
Characteristics of electron beam irradiation to obtain thin biological standards for X-ray peak intensity difference between background intensity determined by comparing the measured excitation thin biological specimens
The content of each element within the region.
4 instruments and equipment
--- Transmission electron microscope;
--- X-ray spectrometer;
--- Ultramicrotome.
5 thin biological standards for selection
5.1 thin biological standards for chemical composition should be as similar as possible with thin biological samples and chemical composition constant.
5.2 thin biological standards for the thickness of the analyzed thickness thin biological specimens should be similar, recommended ultramicrotome into a thickness less than
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