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GB/T 18648-2020: Diagnostic techniques for African swine fever
Status: Valid GB/T 18648: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 18648-2020 | English | 489 |
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Diagnostic techniques for African swine fever
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GB/T 18648-2020
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| GB/T 18648-2002 | English | 279 |
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Diagnostic techniques for African swine fever
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GB/T 18648-2002
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PDF similar to GB/T 18648-2020
Basic data | Standard ID | GB/T 18648-2020 (GB/T18648-2020) | | Description (Translated English) | Diagnostic techniques for African swine fever | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 26,275 | | Date of Issue | 2020-12-14 | | Date of Implementation | 2020-12-14 | | Older Standard (superseded by this standard) | GB/T 18648-2002 | | Quoted Standard | GB 19489 | | Regulation (derived from) | National Standard Announcement No. 28 of 2020 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | | Summary | This standard specifies the clinical diagnosis of ASF, the collection and processing of laboratory diagnostic samples, as well as common PCR methods, fluorescent PCR methods, fluorescent RAA methods, high-sensitivity fluorescent immunoassay methods, sandwich ELISA antigen detection methods, indirect ELISA antibody detection methods, blocking ELISA antibody detection method, sandwich ELISA antibody detection method, indirect immunofluorescence method and other laboratory diagnostic methods. This standard applies to the diagnosis and monitoring of ASF in domestic pigs and wild boars. | GB/T 18648-2020: Diagnostic techniques for African swine fever---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(African swine fever diagnostic technology)
ICS 11:220
B41
National Standards of People's Republic of China
Replace GB/T 18648-2002
African swine fever diagnostic technology
2020-12-14 release
2020-12-14 implementation
State Administration for Market Regulation
Issued by the National Standardization Management Committee
Table of contents
Preface Ⅴ
1 Scope 1
2 Normative references 1
3 Abbreviations 1
4 Biosecurity measures 1
5 Clinical diagnosis 1
5:1 Susceptible animals and hosts 1
5:2 Clinical manifestations 2
5:3 Pathological changes 2
5:4 Judgment of clinical diagnosis results 2
6 Laboratory diagnostic sample collection and processing 2
6:1 Reagent 2
6:2 Sampling equipment 2
6:3 Sample collection 2
6:4 Sample processing 3
7 General PCR method 3
7:1 Reagent 3
7:2 Equipment 3
7:3 Primer sequence 4
7:4 Test procedure 4
7:5 Test establishment conditions 4
7:6 Judgment of ordinary PCR results 5
8 Fluorescence PCR method 5
8:1 Reagent 5
8:2 Instruments and equipment 5
8:3 Primers and probes 5
8:4 Test procedure 5
8:5 Test establishment conditions 6
8:6 Judgment of fluorescent PCR results 6
9 Fluorescence RAA method 6
9:1 Reagent 6
9:2 Instruments and equipment 6
9:3 Primer 6
9:4 Test procedure 6
9:5 Test establishment conditions 7
9:6 Determination of Fluorescence RAA Results 7
10 High-sensitivity fluorescence immunoassay 7
10:1 Reagent 7
10:2 Instruments and equipment 7
10:3 Test procedure 8
10:4 Judgment of high-sensitivity fluorescence immunoassay results 8
11 Sandwich ELISA antigen detection method 9
11:1 Reagent 9
11:2 Instruments and equipment 9
11:3 Test procedure 9
11:4 Test establishment conditions 10
11:5 Determination of antigen test results by sandwich ELISA 10
12 Indirect ELISA antibody detection method 10
12:1 Reagent 10
12:2 Instruments and Equipment 10
12:3 Test procedure 10
12:4 Test establishment conditions 11
12:5 Judgment of indirect ELISA antibody test results 11
13 Blocking ELISA antibody detection method 12
13:1 Reagents 12
13:2 Instruments and Equipment 12
13:3 Test procedure 12
13:4 Calculation method of blocking rate 13
13:5 Test establishment conditions 13
13:6 Judgment of blocking ELISA antibody test results 13
14 Sandwich ELISA antibody detection method 13
14:1 Reagent 13
14:2 Instruments and Equipment 14
14:3 Test procedure 14
14:4 Test establishment conditions 15
14:5 Evaluation of Sandwich ELISA Antibody Test Results 15
15 Indirect immunofluorescence method 15
15:1 Reagent 15
15:2 Equipment 16
15:3 Test procedure 16
15:4 Test establishment conditions 16
15:5 Indirect immunofluorescence result judgment 17
16 Comprehensive decision 17
Appendix A (Normative Appendix) Preparation method of sample preservation solution 18
Appendix B (Normative Appendix) Preparation method of polymerase chain reaction solution 19
Appendix C (Normative Appendix) Preparation method of enzyme-linked immunosorbent test solution 20
Foreword
This standard was drafted in accordance with the rules given in GB/T 1:1-2009:
This standard replaces GB/T 18648-2002 "African swine fever diagnostic technology", compared with GB/T 18648-2002, except for editorial changes
In addition, the main technical changes are as follows:
---Added abbreviations (see Chapter 3);
---Added biosecurity measures (see Chapter 4);
---Added clinical diagnosis (see Chapter 5);
---Added laboratory diagnostic sample collection and processing (see Chapter 6);
---Added the fluorescent PCR method (see Chapter 8), the fluorescent RAA method (see Chapter 9), and the highly sensitive fluorescent immunoassay method (see Chapter 10):
Chapter), sandwich ELISA antigen detection method (see Chapter 11), blocking ELISA antibody detection method (see Chapter 13), sandwich ELISA
ELISA antibody detection methods (see Chapter 14), indirect immunofluorescence methods (see Chapter 15) and other laboratory diagnostic methods;
--- Added comprehensive judgment (see Chapter 16);
---Added the preparation method of sample preservation solution (see Appendix A) and the preparation method of polymerase chain reaction solution (see Appendix B);
---Modified the preparation method of enzyme-linked immunosorbent assay solution (see Appendix C, Appendix C of the:2002 edition):
Please note that certain contents of this document may involve patents: The issuing agency of this document is not responsible for identifying these patents:
This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China:
This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181):
This standard was drafted: China Animal Health and Epidemiology Center:
The main drafters of this standard: Wu Xiaodong, Li Lin, Hu Yongxin, Fan Xiaoxu, Zou Yanli, Ren Weijie, Zhang Yongqiang, Ge Shengqiang, Wang Qinghua,
Li Jinming, Bao Jingyue, Zhao Yonggang, Xu Tiangang, Li Ling, Liu Shan, Jiang Zhengjun, Wang Shushuang, Ma Hongchao, Wang Zhiliang:
The previous versions of the standard replaced by this standard are as follows:
---GB/T 18648-2002:
African swine fever diagnostic technology
1 Scope
This standard specifies the clinical diagnosis of ASF, the collection and processing of laboratory diagnostic samples, as well as common PCR methods and fluorescent PCR methods:
Method, fluorescent RAA method, high-sensitivity fluorescent immunoassay method, sandwich ELISA antigen detection method, indirect ELISA antibody detection method, blocking
Laboratory diagnostic methods such as ELISA antibody detection methods, sandwich ELISA antibody detection methods, and indirect immunofluorescence methods:
This standard applies to the diagnosis and monitoring of ASF in domestic pigs and wild boars:
2 Normative references
The following documents are indispensable for the application of this document: For dated reference documents, only the dated version applies to this article
Pieces: For undated references, the latest version (including all amendments) applies to this document:
GB 19489 Laboratory Biosafety General Requirements
3 Abbreviations
The following abbreviations apply to this document:
ASF: African Swine Fever (AfricanSwineFever)
ASFV: African swine fever virus (AfricanSwineFeverVirus)
DEPC: Diethyl Pyrocarbonate (DiethyPyrocarbonate)
EDTA: Ethylenediaminetetraacetic Acid (Ethylenediaminetetraacetic Acid)
ELISA: Enzyme Linked Immunosorbent Assay (EnzymeLinkedImmunosorbentAssay)
FAM: 6-Carboxy-Fluorescein (6-Carboxy-Fluorescein)
OD: Optical Density (OpticalDensity)
PBS: Phosphate Buffered Saline (PhosphateBufferedSaline)
PCR: Polymerase Chain Reaction (PolymeraseChain Reaction)
RAA: Recombinase-mediated isothermal nucleic acid amplification technology (RecombinaseAidedAmplification)
SPF: No specific pathogen (SpecificPathogenFree)
TMB: Tetramethyl-Benzidine (3,3',5,5'-Tetramethyl-Benzidine)
4 Biosecurity measures
When performing ASF laboratory diagnosis, such as sample processing, nucleic acid extraction, etc:, should be implemented in accordance with GB 19489:
5 Clinical diagnosis
5:1 Susceptible animals and hosts
Swines are susceptible to ASFV: Domestic pigs and Eurasian wild pigs are highly susceptible to ASFV and show similar clinical symptoms and
Mortality; African wild pigs, such as warthogs, bush pigs, red river pigs, and giant forest pigs, have little or no clinical symptoms after infection with ASFV:
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