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GB/T 18649-2024: Diagnostic techniques for contagious bovine pleuropneumonia
Status: Valid GB/T 18649: Evolution and historical versions
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| GB/T 18649-2024 | English | 439 |
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Diagnostic techniques for contagious bovine pleuropneumonia
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GB/T 18649-2024
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| GB/T 18649-2014 | English | 529 |
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Diagnostic techniques for contagious bovine pleuropneumonia
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GB/T 18649-2014
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| GB/T 18649-2002 | English | 439 |
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Diagnostic techniques for contagious bovine pleuropneumonia
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GB/T 18649-2002
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Basic data | Standard ID | GB/T 18649-2024 (GB/T18649-2024) | | Description (Translated English) | Diagnostic techniques for contagious bovine pleuropneumonia | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 22,235 | | Date of Issue | 2024-12-31 | | Date of Implementation | 2025-07-01 | | Older Standard (superseded by this standard) | GB/T 18649-2014 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 18649-2024: Diagnostic techniques for contagious bovine pleuropneumonia---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
National Standard of the People's Republic of China
ICS 11.220CCS B 41
Diagnostic Techniques of Contagious Bovine Pleuropneumonia
2024-12-31 Release
2025-07-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Replaces GB/T 18649-2014
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
This document replaces GB/T 18649-2014 Diagnostic technology for bovine contagious pleuropneumonia.
In addition to structural adjustments and editorial changes, the main technical changes are as follows.
a) The scope has been changed (see Chapter 1, Chapter 1 of the.2014 edition);
b) Change the name of the pathogen of contagious bovine pleuropneumonia to “Mycoplasma mycoides subsp. mycoides” (see Chapter 3,.2014 edition).
Chapter 5);
c) Increased laboratory biosafety measures (see Chapter 5);
d) Deleted “Epidemiology”, “Clinical Symptoms” and “Pathological Changes” (see Chapters 2 to 4 of the.2014 edition);
e) Clinical diagnosis has been added (see Chapter 6);
f) Added sample collection, storage and processing (see Chapter 7);
g) The pathogen isolation and identification was changed, and the contents of specimen collection and biochemical characteristics were deleted (see Chapter 8,.2014 edition).
Chapter 5);
h) Added competition ELISA experiment (see Chapter 11);
i) The overall judgment has been changed (see Chapter 12, Chapter 9 of the.2014 edition);
j) Deleted the sugar culture tube, lead acetate paper strip, nitrate reduction test medium and indicator, indigo test medium and indicator
reagent, methyl red (MR) test medium and indicator, Verpetrol (V-P) test culture grade, barbiturate buffer (VB),
Preparation of Aldrich's solution and determination of complement fixation percentage (see Appendix B to Appendix I and Appendix N of the.2014 edition);
k) The reagent preparation was changed and the preparation of mycoplasma culture medium was moved to it (see Appendix B, Appendix A of the.2014 edition);
l) The preparation of 6% sheep red blood cells, hemolysin titer determination, complement titer determination and antigen titer determination were combined into "complement fixing
Determination of the potency of test reagents” (see Appendix D, Appendix J~Appendix M of the.2014 edition).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Technical Committee on Animal Health Standardization (SAC/TC 181).
This document was drafted by. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shenzhen Green Poetry Source Biotechnology Co., Ltd., China Agricultural
University.
The main drafters of this document are. Xin Jiuqing, Li Yuan, Wang Xiumei, Xu Qingyuan, Yu Haifeng, Xu Fang and Wu Wenxue.
The previous versions of this document and the documents it replaces are as follows.
--First published in.2002 as GB/T 18649-2002 and first revised in.2014;
--This is the second revision.
Introduction
Contagious Bovine Pleuropneumonia (CBPP) is caused by Mycoplasma mycoides subspecies
Plasma mycoides subsp. Mycoides (Mmm) is an infectious disease of cattle, also known as bovine pleuropneumonia.
The World Organization for Animal Health (WOAH) lists it as a notifiable disease.
The Ministry of Agriculture and Rural Affairs of my country listed it as a Class I animal infectious disease in the "List of Class I, II and III Animal Epidemic Diseases" revised in 2022.
Animal diseases.
In 1960, my country successfully developed a highly effective attenuated vaccine. Through large-scale, high-density vaccination combined with slaughtering, quarantine and other
After 1989, no clinical cases were found, and the disease was finally eradicated.
CBPP pest-free certification, becoming a world-recognized CBPP pest-free country.
Pathogen isolation, polymerase chain reaction (PCR), complement fixation test (CFT), competitive enzyme-linked immunosorbent assay (ELISA)
Laboratory testing methods such as ELISA are applied to different scenarios given in Appendix A.
Diagnostic Techniques of Contagious Bovine Pleuropneumonia
1 Scope
This document describes the epidemiology, clinical symptoms, pathological changes, sample collection, storage and processing, and pathogen isolation and identification of CBPP.
Diagnostic methods include PCR detection, CFT detection, competitive ELISA test, etc.
This document applies to the diagnosis, quarantine, surveillance and epidemiological investigation of CBPP.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB 19489 General requirements for laboratory biosafety
NY/T 541 Technical Specification for Collection, Storage and Transportation of Veterinary Diagnostic Samples
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Abbreviations
The following abbreviations apply to this document.
CBPP. Contagious Bovine Pleuropneumonia
CFT. Complement Fixation Test
DNA. Deoxyribonucleic Acid
ELISA. Enzyme Linked Immunosorbent Assay
Mmm. Mycoplasma mycoides subsp. Mycoides
OD value. Optical Density
PBS. Phosphate Buffered Saline
PCR. Polymerase Chain Reaction
5 Laboratory biosafety measures
After the suspected CBPP infection is determined in the sick cattle, the relevant diseased materials will be sent to the laboratory approved by the agricultural administrative department for further analysis.
Pathogen isolation and identification. When conducting CBPP laboratory diagnosis, it shall be carried out in accordance with the provisions of GB 19489 and the Animal Pathogenic Microorganisms.
According to the relevant requirements of the "Detailed Rules for Biosafety Requirements for Experimental Activities", pathogen isolation and identification must be carried out in a biosafety level 3 laboratory.
The collection, storage and transportation shall comply with the requirements of NY/T 541.The water used in the preparation of various reagents in this document shall comply with the requirements of GB/T 6682.
Laboratories approved by the agricultural administrative department should not engage in the isolation and cultivation of this pathogen.
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