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GB/T 18645-2020: Diagnostic techniques for animal tuberculosis
Status: Valid GB/T 18645: Evolution and historical versions
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| GB/T 18645-2020 | English | 499 |
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Diagnostic techniques for animal tuberculosis
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GB/T 18645-2020
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| GB/T 18645-2002 | English | 519 |
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Diagnostic techniques for tuberculosis of animal
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GB/T 18645-2002
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PDF similar to GB/T 18645-2020
Basic data | Standard ID | GB/T 18645-2020 (GB/T18645-2020) | | Description (Translated English) | Diagnostic techniques for animal tuberculosis | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 27,285 | | Date of Issue | 2020-12-14 | | Date of Implementation | 2020-12-14 | | Older Standard (superseded by this standard) | GB/T 18645-2002 | | Quoted Standard | GB/T 18088; GB 19489 | | Regulation (derived from) | National Standard Announcement No. 28 of 2020 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | | Summary | This standard specifies the clinical diagnosis, bacteriological examination, intradermal allergy, PCR and in vitro detection of interferon-r (IFN-r) technical methods, operating procedures and criteria for animal tuberculosis. This standard applies to the diagnosis of animal tuberculosis. The epidemiological characteristics, clinical symptoms, pathological changes and intradermal allergies are applicable to the clinical diagnosis of animal tuberculosis; staining microscopy in bacteriological examination is applicable to the preliminary diagnosis of animal tuberculosis and bacteriological examination Isolation, culture and biochemical identification, pathogen isolation and PCR test are suitable for the pathogenic diagnosis of animal tuberculosis; interferon-r (IFN-r) in vitro detection method is suitable for auxiliary diagnosis of bovine tuberculosis. | GB/T 18645-2020: Diagnostic techniques for animal tuberculosis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Animal tuberculosis diagnosis technology)
ICS 11:220
B41
National Standards of People's Republic of China
Replace GB/T 18645-2002
Animal tuberculosis diagnosis technology
2020-12-14 release
2020-12-14 implementation
State Administration for Market Regulation
Issued by the National Standardization Management Committee
Table of contents
Preface Ⅲ
Introduction Ⅳ
1 Scope 1
2 Normative references 1
3 Abbreviations 1
4 Clinical diagnosis 1
4:1 Popular features 1
4:2 Clinical symptoms 2
4:3 Pathological changes 2
5 Bacteriological examination 2
5:1 Equipment 2
5:2 Reagent 2
5:3 Collection, transportation and treatment of disease materials 2
5:4 Staining microscopic examination 3
5:5 Separation culture and biochemical identification 4
6 Tuberculin intradermal allergy test 5
6:1 Equipment 5
6:2 Reagent 5
6:3 Intradermal allergy of Mycobacterium bovis PPD 5
6:4 Intradermal allergy of Mycobacterium avian PPD 6
6:5 Other animal tuberculosis intradermal allergies 6
7 PCR detection 6
7:1 Equipment 6
7:2 Reagent 6
7:3 Sampling and sample handling 7
7:4 PCR amplification reaction 7
7:5 Detection of PCR amplified products by electrophoresis 8
7:6 Quality Control 8
7:7 Result analysis and judgment 8
7:8 Confirmation of PCR test results 8
8 Interferon-γ (IFN-γ) in vitro detection method (in vitro IFN-γ release test detection method) 8
8:1 Equipment 8
8:2 Reagent 8
8:3 Operation method 8
9 Judgment of diagnosis results 10
Appendix A (informative appendix) Reagent formulation 11
Appendix B (informative appendix) Specimen digestion and concentration method 17
Appendix C (Normative Appendix) Sampling and sample handling (for PCR detection) 18
Appendix D (informative appendix) DNA extraction solution 20
Appendix E (informative appendix) Nucleic acid sequences of conventional PCR amplification products 21
Appendix F (Normative Appendix) Requirements for sample collection, packaging and transportation 22
Foreword
This standard was drafted in accordance with the rules given in GB/T 1:1-2009:
This standard replaces GB/T 18645-2002 "Animal Tuberculosis Diagnostic Technology": Compared with GB/T 18645-2002, the main technology
The content changes are as follows:
---Added the introduction;
---Added normative reference documents (see Chapter 2);
---Added abbreviations (see Chapter 3);
---Added clinical diagnosis methods, including epidemic characteristics, clinical symptoms and pathological changes (see 4:1, 4:2 and 4:3);
---Added warning content, for necropsy, sampling and laboratory related pathogen detection part and laboratory contact with toxic substances
Give a warning (see 4:3);
--- Increased intradermal allergies in other animals (see 6:5);
--- PCR detection has been added (see Chapter 7);
---Added γ-interferon (IFN-γ) in vitro detection method (see Chapter 8);
---Added the diagnosis result judgment (see Chapter 9);
--- Deleted the content of animal vaccination test (see 2:3:1 of the:2002 edition);
---Added the nucleic acid sequence of the conventional PCR amplification product (see Appendix E);
---Added sample collection, packaging and transportation requirements (see Appendix F):
Please note that certain contents of this document may involve patents: The issuing agency of this document is not responsible for identifying these patents:
This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China:
This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181):
This standard was drafted: China Veterinary Drug Supervision Institute, China Animal Health and Epidemiology Research Center:
The main drafters of this standard: Zhu Liangquan, Ding Jiabo, Shen Qingchun, Sun Mingjun, Mao Kairong, Wei Rong, Xu Guanlong, Feng Yu:
The previous published versions of the standard replaced by this standard are:
---GB/T 18645-2002:
Introduction
Animal tuberculosis is a chronic and zoonotic infectious disease caused by bacteria of the genus Mycobacterium, which is a serious threat worldwide
Human health and affect the development of animal husbandry: Mycobacterium is mainly composed of Mycobacterium tuberculosis complex, Mycobacterium leprae and non-tuberculous
Bacillus composition: And Mycobacterium bovis and Mycobacterium tuberculosis are members of the Mycobacterium tuberculosis complex, and Mycobacterium avian is a non-tuberculous branch
Bacillus member: Bovine tuberculosis is a national second-class animal disease, and it is also a 16 species in the National Medium and Long-term Animal Disease Prevention and Control Plan (2012-2020)
One of the domestic animal diseases that are given priority to control, the main pathogen is Mycobacterium bovis: The host spectrum of Mycobacterium bovis is broad, including almost all warm
Blood vertebrates: In addition to cattle being the most susceptible, it can also infect many animals such as deer and pigs; Mycobacterium tuberculosis mainly causes human tuberculosis, but it can also be infected
Cattle; Avian mycobacterium avian subspecies mainly causes avian tuberculosis:
Animal tuberculosis is mainly infected through the respiratory and digestive tracts, which can affect a variety of animals: Dairy cows are the most susceptible among domestic animals, followed by cattle and yak:
Cattle, buffalo, pig and poultry, deer are more common in wild animals: The main clinical features of the disease are slow course, progressive weight loss, coughing, and depression
Exhausted, and form granulomas and caseous calcified nodules in a variety of tissues and organs (such as lung, liver, spleen and intestines, etc:):
In recent years, domestic and international attention has been paid to the prevention and control of animal tuberculosis: For example, bovine tuberculosis, which has a high incidence and more serious damage, has been recognized by the world animal health
The Organization (OIE) is listed as a disease that must be notified, and it is also a subject of international trade inspection: With the vigorous development of international trade, animal tuberculosis
And spread among many kinds of animals, seriously endangering animal husbandry and public health safety: The current diagnostic technology of animal tuberculosis in my country
The standard is GB/T 18645-2002 "Animal Tuberculosis Diagnosis Technology", which is limited by the knowledge and technical conditions at the time, and the content cannot meet the current requirements:
Former animal tuberculosis diagnosis, quarantine and testing needs: There are mainly the following outstanding problems: 1) For countries where zoonotic tuberculosis is diagnosed
Standards, lack of necessary biosafety requirements, and clinical diagnosis related content such as epidemiology, clinical symptoms and pathological changes: 2) Diagnosis
The method involves the content of animal inoculation experiments: Due to the long diagnosis time (at least 1 month) and animal welfare factors, it is rarely used internationally:
3) The diagnosis method lacks new universal mature technology (such as PCR, in vitro detection of interferon gamma, etc:): 4) Need to test the niacin involved in the standard
The biochemical methods for testing or nitrobenzoic acid testing are standardized and unified: Such as GB/T 18645-2002 "Animal Tuberculosis Diagnostic Technology" and
SN/T 1310-2011 "Technical Specifications for Animal Tuberculosis Inspection and Quarantine" involves inconsistent biochemical test methods: The former uses niacin to test poison
The latter is relatively safe using the p-nitrobenzoic acid test: 5) The scope of application needs to be increased to meet the needs of entry-exit inspection and quarantine work:
Needs of development: Therefore, the revision and improvement of the national standards applicable to the diagnosis of animal tuberculosis in our country are important for clinical veterinary, inspection and quarantine and other scientific research workers:
It is of great significance for the author to make a timely and accurate diagnosis, so as to take rapid, reasonable and effective response measures to epidemic prevention and control and epidemic disposal:
Animal tuberculosis diagnosis technology
1 Scope
This standard specifies the clinical diagnosis, bacteriological examination, intradermal allergy, PCR and interferon-γ (IFN-γ) in vitro for animal tuberculosis
Technical methods, operating procedures and judgment standards for testing:
This standard applies to the diagnosis of animal tuberculosis, in which the epidemic characteristics, clinical symptoms, pathological changes and intradermal allergies are applicable to animals:
Clinical diagnosis of biological tuberculosis; staining microscopy in bacteriological examination is suitable for preliminary diagnosis of animal tuberculosis etiology, and separation and culture in bacteriological examination
Nutritional and biochemical identification, pathogen isolation and PCR test are applicable to the pathogenic diagnosis of animal tuberculosis; the in vitro detection method of interferon-γ (IFN-γ) is applicable
For the auxiliary diagnosis of bovine tuberculosis:
2 Normative references
The following documents are indispensable for the application of this document: For dated reference documents, only the dated version applies to this article
Pieces: For undated references, the latest version (including all amendments) applies to this document:
GB/T 18088 Entry and Exit Animal Quarantine Sampling
GB 19489 Laboratory Biosafety General Requirements
3 Abbreviations
The following abbreviations apply to this document:
OIE: World Organization for Animal Health (WorldOrganizationforAnimalHealth)
PPD: Purified Protein Derivative (PurifiedProteinDerivative)
IU: International Unit (InternationalUnit)
PCR: Polymerase Chain Reaction (PolymeraseChain Reaction)
ELISA: Enzyme-linked Immuno-sorbent Assay (Enzyme-linkedImmuno-sorbentAssay)
TCH: Thiophen-2-carboxylic Acid Hydrazide (Thiophen-2-carboxylic Acid Hydrazide)
IFN-γ: Gamma Interferon (GammaInterferon)
PNB: p-nitrobenzoic acid (P-nitrobenzoicAcid)
DNA: Deoxyribonucleic Acid (Deoxyribonucleic Acid)
dNTPs: Deoxy-Ribonucleoside Triphosphate
EDTA: EthyleneDiamineTetraacetic Acid (EthyleneDiamineTetraacetic Acid)
4 Clinical diagnosis
4:1 Popular features
A variety of animals are susceptible to Mycobacterium bovis, Mycobacterium tuberculosis, and Mycobacterium avian: Among the animals, dairy cows are the most susceptible, followed by cattle, yak,
Buffalo, pig and poultry, deer are more common in wild animals: Mycobacterium tuberculosis mainly affects humans, and is rare in cattle and pigs; Mycobacterium bovis mainly
It can infect cattle, and can also infect humans, sheep, goats, pigs and dogs; Mycobacterium avium mainly affects poultry and waterfowl, of which chickens and pigeons are the most susceptible, geese and
Ducks are followed by cattle, pigs and humans: Among experimental animals, guinea pigs are sensitive to Mycobacterium bovis and Mycobacterium tuberculosis, but are resistant to Mycobacterium avian
Resistance, usually can only form local lesions; rabbits are highly sensitive to Mycobacterium avian and Mycobacterium bovis, although Mycobacterium tuberculosis can cause home
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