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GB/T 18644-2020: Diagnostic techniques for porcine cysticercosis
Status: Valid GB/T 18644: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 18644-2020 | English | 489 |
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Diagnostic techniques for porcine cysticercosis
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GB/T 18644-2020
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| GB/T 18644-2002 | English | 239 |
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Diagnostic techniques for cysticercosis cellulosae
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GB/T 18644-2002
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PDF similar to GB/T 18644-2020
Basic data | Standard ID | GB/T 18644-2020 (GB/T18644-2020) | | Description (Translated English) | Diagnostic techniques for porcine cysticercosis | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 26,255 | | Date of Issue | 2020-12-14 | | Date of Implementation | 2020-12-14 | | Older Standard (superseded by this standard) | GB/T 18644-2002 | | Regulation (derived from) | National Standard Announcement No. 28 of 2020 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 18644-2020: Diagnostic techniques for porcine cysticercosis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Diagnosis technology of swine cysticercosis)
ICS 11:220
B41
National Standards of People's Republic of China
Replace GB/T 18644-2002
Diagnosis technology of swine cysticercosis
2020-12-14 release
2020-12-14 implementation
State Administration for Market Regulation
Issued by the National Standardization Management Committee
Table of contents
Preface Ⅲ
Introduction Ⅳ
1 Scope 1
2 Normative references 1
3 Abbreviations 1
4 Microscopic examination 1
4:1 Reagent 1
4:2 Equipment 1
4:3 Insect collection 2
4:4 Tablet preparation 2
4:5 Microscopic examination 2
4:6 Judgment of Microscopic Inspection Results 2
5 PCR method 2
5:1 Reagent 2
5:2 Equipment 2
5:3 Primer 3
5:4 Sample 3
5:5 PCR procedure 3
5:6 Electrophoresis detection of amplified products 4
5:7 Test establishment conditions 4
5:8 Determination of PCR results 4
6 Indirect ELISA 4
6:1 Reagent 4
6:2 Instruments and equipment 4
6:3 Sample 5
6:4 Test Step 5
6:5 Test establishment conditions 6
6:6 Determination of ELISA results 6
7 Dot-ABC-ELISA 6
7:1 Reagent 6
7:2 Instruments and equipment 7
7:3 Sample 7
7:4 Test Step 7
7:5 Test establishment conditions 8
7:6 Dot-ABC-ELISA result judgment 8
8 Comprehensive decision 8
Appendix A (normative appendix) Physiological saline and morphological characteristics of cysticercus sacrum
Appendix B (Normative Appendix) PCR primer location, solution preparation and electrophoresis results 10
Appendix C (Normative Appendix) ELISA reagents and their preparation 12
Appendix D (Informative Appendix) Preparation of Cysticercus cellulosae TS-CC18 recombinant protein 14
Appendix E (informative appendix) Sample addition of indirect enzyme-linked immunosorbent assay 16
Appendix F (Informative Appendix) Preparation of Cysticercus cellulosae TS-CC18 and Enolase Monoclonal Antibody 17
Appendix G (informative appendix) Dot-ABC-ELISA related reagent preparation and result judgment 20
Foreword
This standard was drafted in accordance with the rules given in GB/T 1:1-2009:
This standard replaces GB/T 18644-2002 "Cysticercus cellulosae diagnosis technology", compared with GB/T 18644-2002, except for editorial revision
In addition, the main technical changes are as follows:
---The scope part adds PCR and Dot-ABC-ELISA technology (see Chapter 1);
--- Added abbreviations (see Chapter 3);
---Added PCR detection method for pathogens (see Chapter 5);
---Modified the detection antigen and detection steps of the enzyme-linked immunosorbent assay, and directly performed antibody detection on serum samples (see Chapter 6,
Chapter 3 of the:2002 edition);
---Added Dot-ABC-ELISA method for detecting circulating antigens (see Chapter 7);
--- Added comprehensive judgment (see Chapter 8);
---Added pictures of normal saline and cysticercus sacrum (see Appendix A), PCR primer positions, solution preparation and electrophoresis pictures (see attached
Record B), ELISA reagents and their preparations (see Appendix C, Appendix A of the:2002 edition), the recombinant protein of Cysticercus cellulosae TS-CC18
Preparation (see Appendix D), sample addition example for indirect enzyme-linked immunosorbent assay (see Appendix E), Cysticercus cellulosae TS-CC18 and enolization
Enzyme monoclonal antibody preparation (see Appendix F), Dot-ABC-ELISA related reagent preparation and result determination (see Appendix G):
Please note that certain contents of this document may involve patents: The issuing agency of this document is not responsible for identifying these patents:
This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China:
This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181):
This standard was drafted: Lanzhou Institute of Veterinary Medicine, Chinese Academy of Agricultural Sciences:
The main drafters of this standard: Cai Xuepeng, Luo Xuenong, Zhang Shaohua, Zheng Yadong, Guo Aijiang, Hou Junling:
The previous versions of the standard replaced by this standard are as follows:
---GB/T 18644-2002:
Introduction
Porcine cysticercosis (porcinecysticercosis) is a serious damage caused by the larva of Taeniasolium
Zoonotic parasitic diseases: The World Animal Health Organization lists the disease as one of the diseases to be declared: Currently, the disease is widespread in development
The number of cases in countries and developed countries is also increasing year by year, which not only affects the development of the pig industry and causes huge economic losses, but also
A serious threat to human health:
The diagnosis of swine cysticercosis includes pathogenic diagnosis and serological diagnosis: The purpose of pathogenic diagnosis is to determine the worms in clinical necropsy samples
The morphological characteristics of the body can be judged as Cysticercus cellulosae by observing under a microscope, there are two neatly arranged small hooks on the top of the head segment: Cysticercus
It is mainly parasitic in the liver, so it should be distinguished from the cysticercus of Taenia asiatica: At present, the pre-mortem diagnosis of the disease mainly uses serological methods:
The antigens used include worm antigens, cyst fluid antigens, or secreted metabolic antigens: Although these antigens have high sensitivity, they have poor specificity:
And the source of antigen is very limited: In view of the above reasons, this revision of GB/T 18644-2002 "Cysticercus cellulosae Diagnosis Technology", except for some
The antigen used in ELISA was changed to a recombinant protein of Cysticercus cellulosae stage specific 18ku gene (TS-CC18), and serum circulating antigen was added
(CAg) detection methods and pathogen PCR detection methods to meet the epidemiological investigation of cysticercosis, drug treatment effect evaluation and animal
Different needs such as circulation risk assessment:
The issuing authority of this document draws attention to the fact that when a declaration conforms to this document, Chapter 6 Indirect ELISA may be involved:
(ELISA) related patent use:
The issuing agency of this document has no position on the authenticity, validity and scope of the patent:
The patent holder has assured the issuing organization of this document that he is willing to work with any applicant under reasonable and non-discriminatory terms and conditions:
Negotiations on patent licensing: The statement of the patent holder has been filed with the issuing agency of this document: Relevant information can be through the following
Contact information:
Names of patent holders: Cai Xuepeng, Zhang Shaohua, Jing Zhizhong, Luo Xuenong, Guo Aijiang, Zheng Yadong, Dou Yongxi:
Address: No: 1, Xujiaping, Yanchangbao, Lanzhou City, Gansu Province:
Please note that in addition to the above patents, certain contents of this document may still involve patents: The issuing agency of this document is not responsible for identifying these
Li’s responsibility:
Diagnosis technology of swine cysticercosis
1 Scope
This standard specifies the microscopic examination, polymerase chain reaction method (PCR method), indirect enzyme-linked immunosorbent test (intermediate enzyme-linked immunosorbent assay) of swine cysticercosis
Connected with ELISA), dot biotin-avidin complex enzyme-linked immunosorbent test (Dot-ABC-ELISA) diagnostic technology and comprehensive judgment:
This standard applies to the diagnosis of cysticercosis in pigs and wild boars:
2 Normative references
The following documents are indispensable for the application of this document: For dated reference documents, only the dated version applies to this article
Pieces: For undated references, the latest version (including all amendments) applies to this document:
CNAS-GL029:2018 Guidelines for Accreditation of Testing Laboratories in the Field of Gene Amplification
3 Abbreviations
The following abbreviations apply to this document:
Avidin-HRP: Avidin-Horseradish Peroxidase (Avidin-Horseradish Peroxidase)
DAB: Diaminobenzidine (3,3'-Diaminobenzidine)
Dot-ABC-ELISA: dot biotin-avidin complex enzyme-linked immunosorbent assay (Dot-Avidin-Biotin-Complex-
ELISA)
ELISA: Enzyme-LinkedImmunosorbentAssay
HRP: Horseradish Peroxidase (HorseRadish Peroxidase)
IPTG: Isopropyl β-D-Thiogalactoside (Isopropyl β-D-Thiogalactoside)
NC: Nicrocelulose
OPD: O-phenylenediamine
PBS: Phosphate Buffered Saline (PhosphateBufferedSaline)
PBST: Washing solution (PBScontaining0:5% Tween-20)
PCR: Polymerase Chain Reaction (PolymeraseChain Reaction)
TMB: TetramethylBenzidine (3,3',5,5'-TetramethylBenzidine)
4 Microscopic examination
4:1 Reagents
For the preparation of physiological saline, see A:1 in Appendix A:
4:2 Equipment
4:2:1 Upright biological microscope:
4:2:2 Surgical scissors, scalpel and forceps:
4:2:3 Glass slides:
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