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GB/T 18642-2021 English PDF

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GB/T 18642-2021: Diagnostic techniques for Trichinella spp.
Status: Valid

GB/T 18642: Evolution and historical versions

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
GB/T 18642-2021	English	489	Add to Cart	5 days [Need to translate]	Diagnostic techniques for Trichinella spp.	Valid	GB/T 18642-2021
GB/T 18642-2002	English	279	Add to Cart	3 days [Need to translate]	Diagnostic techniques for trichinella spiralis in swine	Obsolete	GB/T 18642-2002

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Basic data

Standard ID	GB/T 18642-2021 (GB/T18642-2021)
Description (Translated English)	Diagnostic techniques for Trichinella spp.
Sector / Industry	National Standard (Recommended)
Classification of Chinese Standard	B41
Word Count Estimation	26,282
Issuing agency(ies)	State Administration for Market Regulation, China National Standardization Administration

GB/T 18642-2021: Diagnostic techniques for Trichinella spp.

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic techniques for Trichinella spp. ICS 11.220 B41 National Standards of People's Republic of China Replace GB/T 18642-2002 Trichinella diagnostic technology Released on 2021-04-30 2021-11-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword Ⅲ Introduction Ⅳ 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 Abbreviations 1 5 Tablet microscopy 2 6 Sample digestion method 3 7 Enzyme-linked immunosorbent assay (ELISA method) 4 8 Fluorescence immunochromatography test paper card method 7 9 Multiplex polymerase chain reaction method (multiplex PCR method) 9 10 Comprehensive Judgment 12 Appendix A (Normative Appendix) Diagram of Solution Preparation and Judgment of Compression Microscopy 13 Appendix B (Normative Appendix) Preparation of Digestive Juice 14 Appendix C (informative appendix) Flow chart of Trichinella digestion method 15 Appendix D (Normative appendix) Preparation of solution for enzyme-linked immunosorbent assay 16 Appendix E (Normative Appendix) Preparation of Solution for Fluorescence Immunochromatography Test Paper Card Test 18 Appendix F (informative appendix) Schematic diagram of the structure and determination of the fluorescence immunochromatographic test paper card 19 Appendix G (Normative Appendix) Solution Preparation for Multiplex PCR Method 20 Reference 21

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces GB/T 18642-2002 "Porcine Trichinosis Diagnosis Technology", compared with GB/T 18642-2002, except for editorial amendments The main technical changes of the external change are as follows. ---Added normative reference documents (see Chapter 2); ---Added terms and definitions (see Chapter 3); ---Added abbreviations (see Chapter 4); ---Added the enzyme-linked immunosorbent test (ELISA) diagnostic method and the preparation of the enzyme-linked immunosorbent test solution (see Chapter 7, Appendix Record D); ---Added the diagnostic method of fluorescence immunochromatography test paper card, the preparation of the solution for fluorescence immunochromatography test paper card test, and the fluorescence immunochromatography test Paper card structure and judgment diagram (see Chapter 8, Appendix E and Appendix F); ---Added a multiplex polymerase chain reaction (multiplex PCR) diagnostic method for the identification of Trichinella species, and a multiplex PCR method Liquid preparation (see Chapter 9, Appendix G); --- Added comprehensive judgment (see Chapter 10); ---Added the schematic diagram of solution preparation and judgment of the tablet microscopy method (see Appendix A) ---Added the preparation of digestive juice (see Appendix B); ---Added the flow chart of Trichinella collection sample digestion method (see Appendix C). Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying these patents. This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181). Drafting organizations of this standard. Jilin University, China Center for Animal Health and Epidemiology, China Center for Disease Control and Prevention Control the place. The main drafters of this standard. Liu Mingyuan, Liu Xiaolei, Wang Yuanyuan, Wu Xiuping, Dong Yaqin, Bai Xue, Wang Xuelin, Tang Bin, Yang Yong, Ding Jing. The previous editions of the standard replaced by this standard are as follows. ---GB/T 18642-2002.

Introduction

Trichinellaspp. was first discovered in the corpse of a person in London in 1835 by the British scholar JamesPanesPaget. In the same year, Richard Owen named the pathogen Trichinellaspiralis. At present, 9 species and genus of Trichinella have been identified through species identification. 3 unclear genotypes. Trichinella is a serious zoonotic parasitic disease that can infect humans and more than 150 animals. It is caused by eating raw or semi-raw meat containing live Trichinella spiralis (such as pork, horse meat, dog meat, wild animal meat, etc.). Human trichinosis (HumanTrichinelosis) has an incubation period of 2 to 4 weeks and a mortality rate of 2% to 30%. The disease is recognized by the World Organisation for Animal Health (WorldOrganizationforAnimalHealth, OIE) is listed as a compulsory disease for slaughtered animals (especially pigs and horses). The most expensive zoonotic disease in the world has been invested in control. my country’s "Category I, II, and III Animal Diseases List" lists it as a Category II animal Plague. The revision of this standard refers to the.2017 edition of the OIE "Manual of Diagnostic Tests and Vaccine Standards for Terrestrial Animals" (Manual of Diagnostic Tests and Vaccines). TestsandVaccinesforTerrestrialAnimals,.2017), International Commission on Trichinosis (InternationalCommissionon Trichinelosis, ICT) "Recommendations for Serological Tests to Detect Trichinella Infections in Animals and Humans".2018 Edition (Recommendationson theuseofserologicaltestsforthedetectionofTrichinellainfectioninanimalsandhumans,.2018) and Recommendations for Genotyping Trichinella Muscle Stagelar-2018 (RecommendationsforgenotypingTrichinellamusclestagelar- vae,.2018) related international standards. Serological diagnosis adds two kinds of enzyme-linked immunosorbent test (ELISA) and fluorescence immunochromatographic test paper cards Technology, serological diagnosis technology determines that the sample is positive or suspected to be positive for Trichinella infection can be further confirmed by digestion; molecular biology The diagnosis adds multiple polymerase chain reaction (multiplex PCR) technology. Trichinella diagnostic technology

1 Scope

This standard specifies the pathogenic diagnosis and enzyme-linked immunosorbent test for Trichinella in domestic animals (pigs, horses and canines) and wild animals (ELISA), fluorescence immunochromatography test paper card, multiple polymerase chain reaction (multiplex PCR) detection method technical requirements and operating specifications. This standard applies to pig origin quarantine, slaughter and processing, epidemiological investigation, import and export inspection and quarantine, and other animals and their meat. Trichinella diagnosis. The listed methods include etiology, serology and molecular biology diagnosis, of which. ---The slide microscopy method and the sample digestion method are suitable for the diagnosis of Trichinella in the carcass of pigs and other animals; ---ELISA and fluorescence immunochromatographic test paper card methods are suitable for the screening of Trichinella spiralis in pigs and other animal live bodies and carcasses; ---Multiplex PCR method is suitable for the identification of species and genotypes of Trichinella isolates.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article Pieces. For undated reference documents, the latest version (including all amendments) is applicable to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Pooleddigestion Mix multiple animal muscle samples and dissolve their muscle cells with a certain concentration of pepsin solution under certain temperature and pH conditions. 3.2 Excretion-secretoryproducts Trichinella spiralis secretes various molecular products through the epidermis or excreted through the digestive tract. 3.3 Cysts After Trichinella invades the host muscle cells, a cyst wall composed of collagen fibers is formed around the body to escape the host's immune attack. 3.4 Musclelarvae Trichinella larvae parasitic on the host muscle cells.

4 Abbreviations

The following abbreviations apply to this document. DMEM. Dulbecco's modified Eagle's medium (Dulbecco's modified Eaglemedium) EDC. 1-(3-dimethylaminopropyl)-3-ethylcarbodimide[1-(3-dimethylaminopropyl)-3-ethylcarbodimide] ELISA. enzyme-linked immunosorbent assay (enzymelinked immunosorbent assay)

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