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GB/T 17494-2023: Diagnostic techniques for equine infectious anemia
Status: Valid GB/T 17494: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 17494-2023 | English | 419 |
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Diagnostic techniques for equine infectious anemia
| Valid |
GB/T 17494-2023
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| GB/T 17494-2009 | English | 359 |
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Diagnostic techniques of indirect ELISA technique for equine infectious anemia disease
| Obsolete |
GB/T 17494-2009
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| GB/T 17494-1998 | English | 279 |
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Rules of indirect ELISA technique for equine infectious anemia disease
| Obsolete |
GB/T 17494-1998
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PDF similar to GB/T 17494-2023
Basic data | Standard ID | GB/T 17494-2023 (GB/T17494-2023) | | Description (Translated English) | Diagnostic techniques for equine infectious anemia | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 22,295 | | Date of Issue | 2023-09-07 | | Date of Implementation | 2024-04-01 | | Older Standard (superseded by this standard) | GB/T 17494-2009 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 17494-2023: Diagnostic techniques for equine infectious anemia---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 11.220
CCSB41
National Standards of People's Republic of China
Replace GB/T 17494-2009
Diagnostic Technology for Equine Infectious Anemia
Published on 2023-09-07
2024-04-01 Implementation
State Administration for Market Regulation
Released by the National Standardization Administration Committee
Table of contents
Preface III
Introduction IV
1 Scope 1
2 Normative reference documents 1
3 Terms and Definitions 1
4 Abbreviations 1
5 Laboratory biosafety measures 2
6 Clinical Diagnosis 2
7 Sample collection, storage and transportation3
8 Real-time PCR 4
9 Virus isolation and identification5
10 Agar gel immunodiffusion test 6
11 Indirect ELISA 8
12 Competitive ELISA 10
13 Western blot assay 11
14 Comprehensive judgment 13
Appendix A (normative) Preparation of reagents 14
Appendix B (informative) Real-time fluorescence PCR primers and probes 16
Appendix C (informative) Preparation of real-time fluorescence PCR positive control plasmid 17
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
This document replaces GB/T 17494-2009 "Indirect ELISA Diagnostic Technology for Equine Infectious Anemia" and is the same as GB/T 17494-2009
In comparison, in addition to structural adjustments and editorial changes, the main technical changes are as follows.
--- Added clinical diagnosis of equine infectious anemia (see Chapter 6);
--- Added real-time fluorescence PCR detection of equine infectious anemia virus (see Chapter 8);
---Added the isolation and identification of equine infectious anemia virus (see Chapter 9);
---Added equine infectious anemia agar gel immunodiffusion test (see Chapter 10);
---Changed the indirect ELISA for equine infectious anemia (see Chapter 11, Chapter 4 and 5 of the.2009 edition);
---Added equine infectious anemia competition ELISA (see Chapter 12);
---Added equine infectious anemia immunoblotting test (see Chapter 13);
Please note that some content in this document may be subject to patents. The publisher of this document assumes no responsibility for identifying patents.
This document is proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181).
This document was drafted by. Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Xinjiang Uygur Autonomous Region Animal Disease Prevention and Control Center,
Guangzhou Customs of the People's Republic of China, China Agricultural University, Beijing Customs of the People's Republic of China.
The main drafters of this document. Hu Zhe, Wang Xiaojun, Guo Wei, Lin Yuezhi, Wang Xuefeng, Wang Wen, Chen Ru, Wang Qin, and Bai Yaduo.
The previous versions of this document and the documents it replaces are as follows.
---First published as GB/T 17494-2009 in.2009;
---This is the first revision.
Introduction
Category II animal diseases stipulated in the "List of Epidemic Diseases" (2022).
EIAV only infects animals of the genus Equus, of which horses are the most susceptible. Infected animals are the main source of infection. After EIAV infects the host, the virus will
The body's genome is integrated into the host cell genome, causing persistent host infection and lifelong virus transmission. Clinically, sick animals may show repeated fever,
Anemia, jaundice, hemorrhage, edema and emaciation are the characteristics of the disease. However, a certain proportion of horses and most donkeys and mules do not show obvious clinical symptoms after infection.
As latent virus carriers. There is currently no available vaccine for this disease. The diagnosis of this disease requires laboratory diagnosis. EIAV belongs to reverse transcription
After entering the host cell, the virus and EIAV replicate in the early stage under the action of viral reverse transcriptase to form double-stranded viral DNA (often called provirus).
DNA), the proviral DNA is further integrated into the host chromosome under the action of the virus-encoded integrase. EIAV proviral DNA
It is relatively stable in the infected host and is often used as a target for viral nucleic acid detection. There are relatively few EIAV genome sequences in different regions of the world.
There is a big difference. The nucleic acid detection method used in this document is a universal real-time fluorescence PCR method suitable for different current strains.
Diagnostic Technology for Equine Infectious Anemia
1 Scope
This document describes the clinical diagnosis of EIA, real-time fluorescence PCR, virus isolation and identification, agar gel immunodiffusion test, indirect
Methods of ELISA, competitive ELISA, and western blot assays.
This document applies to the diagnosis, quarantine, surveillance and epidemiological investigation of EIA.
2 Normative reference documents
The contents of the following documents constitute essential provisions of this document through normative citations in the text. Among them, dated references
For referenced documents without dates, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to this document.
document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB 19489 General requirements for laboratory biosafety
NY/T 541 Technical specifications for collection, storage and transportation of veterinary diagnostic samples
3 Terms and definitions
There are no terms or definitions to be defined in this document.
4 Abbreviations
The following abbreviations apply to this document.
BCA. Bicinchoninic Acid
OD value. optical density value (OpticalDensity)
Electrophoresis)
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