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GB/T 15670.20-2017 English PDF

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GB/T 15670.20-2017: Toxicological test methods for pesticides registration -- Part 20: In vitro mammalian cell gene mutation test
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Basic data

Standard ID GB/T 15670.20-2017 (GB/T15670.20-2017)
Description (Translated English) Toxicological test methods for pesticides registration -- Part 20: In vitro mammalian cell gene mutation test
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B17
Classification of International Standard 65.100
Word Count Estimation 9,963
Date of Issue 2017-07-12
Date of Implementation 2018-02-01
Quoted Standard GB/T 15670.14-2017
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the basic principles, methods and requirements for mutations in mammalian cells in vitro. This standard applies to in vitro mammalian cell gene mutation testing for pesticide registration.

GB/T 15670.20-2017: Toxicological test methods for pesticides registration -- Part 20: In vitro mammalian cell gene mutation test


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Toxicological test methods for pesticides registration. Part 20. In vitro mammalian cell gene mutation test ICS 65.100 B17 National Standards of People's Republic of China Pesticide registration Toxicology test methods Part 20. In vitro mammalian cell genes Mutational test Part 20. Invitromammaliancelgenemutationtest 2017-07-12 Posted 2018-02-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

GB/T 15670 "pesticide registration toxicology test method" is divided into the following sections. --- Part 1. General principles; --- Part 2. Acute oral toxicity test Horn's method; --- Part 3. Acute oral toxicity test sequential method; --- Part 4. Acute oral toxicity test probability unit method; --- Part 5. Acute dermal toxicity test; --- Part 6. Acute inhalation toxicity test; --- Part 7. Skin irritation/corrosivity test; --- Part 8. Acute eye irritation/corrosiveness test; --- Part 9. Skin allergy (sensitization) test; --- Part 10. Short-term repeated oral toxicity (28 days) toxicity test; --- Part 11. Short-term repeated transdermal (28 days) toxicity test; --- Part 12. Short-term repeated inhalation exposure (28 days) toxicity test; --- Part 13. Subchronic toxicity test; --- Part 14. bacterial recovery mutation test; --- Part 15. In vivo mammalian bone marrow polychromatic erythrocyte micronucleus test; --- Part 16. In vivo mammalian bone marrow cell chromosome aberration test; --- Part 17. Mammalian spermatogonial/spermatocyte chromosome aberration test; --- Part 18. Rodent dominant lethality test; --- Part 19. In vitro mammalian chromosome aberration test; --- Part 20. In vitro mammalian cell gene mutation test; --- Part 21. In vivo mammalian hepatocyte extracellular DNA synthesis (UDS) test; --- Part 22. DNA damage and repair in vitro mammalian cells/DNA synthesis test outside the program; --- Part 23. Teratogenicity test; --- Part 24. Two generations of reproductive toxicity test; --- Part 25. Acute delayed neurotoxicity test; --- Part 26. Chronic toxicity test; --- Part 27. Carcinogenicity test; --- Part 28. Chronic toxicity and carcinogenicity combined test; --- Part 29. Metabolism and toxicokinetic tests. This section GB/T 15670 Part 20. This section drafted in accordance with GB/T 1.1-2009 given rules. This part is proposed and managed by the Ministry of Agriculture of the People's Republic of China. This part of the drafting unit. Ministry of Agriculture pesticide test. The main drafters of this section. Zhang Hongwei, Zhang Liying, Tao Chuanjiang. Pesticide registration Toxicology test methods Part 20. In vitro mammalian cell genes Mutational test

1 Scope

This section GB/T 15670 specifies the basic principles, methods and requirements of in vitro mammalian cell gene mutation tests. This section applies to the registration of pesticides in vitro mammalian cell gene mutation test.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 15670.14-2017 Pesticide registration Toxicological test methods Part 14. Bacterial back mutation test

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Forward mutation forwardmutation Wild-type gene after mutation into a mutant gene process, this mutation can cause changes in enzymes and functional proteins. 3.2 Base substitutions mutagens basepairsubstitutionmutagens A substance that causes the replacement of one or more base pairs in a DNA molecule. 3.3 Frameshift mutagens A substance that causes the DNA molecule to increase or lose one or more base pairs. 3.4 Phenotypic expression time phenotypicexpressiontime The time required for the newly mutated cells to exhaust the altered gene product. 3.5 Mutant frequency mutantfrequency The ratio of the number of mutant cells observed to the number of surviving cells.

4 test purposes

This test is used to test the effect of the test substance on the gene mutation of mammalian cells cultured in vitro, including base pair mutation, frameshift mutation and Missing, etc. to evaluate the possibility of the test substance causing the mutation.

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