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GB/T 4789.27-2008: Microbiological examination of food hygiene -- Examination of residue of antibiotics in fresh milk
Status: Valid GB/T 4789.27: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 4789.27-2008 | English | 229 |
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Microbiological examination of food hygiene -- Examination of residue of antibiotics in fresh milk
| Valid |
GB/T 4789.27-2008
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| GB/T 4789.27-2003 | English | 199 |
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Microbiological examination of food hygiene -- Examination of residues of residue of antibiotics in fresh milk
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GB/T 4789.27-2003
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| GB 4789.27-1994 | English | RFQ |
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Microbiological examination of food hygiene. Examination of residue of antibiotics in fresh milk
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GB 4789.27-1994
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| GB 4789.27-1984 | English | RFQ |
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Microbiological examination of food hygiene--Examination of the residue of antibiotics in fresh milk
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GB 4789.27-1984
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PDF similar to GB/T 4789.27-2008
Basic data | Standard ID | GB/T 4789.27-2008 (GB/T4789.27-2008) | | Description (Translated English) | Microbiological examination of food hygiene -- Examination of residue of antibiotics in fresh milk | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C53 | | Classification of International Standard | 07.100.30 | | Word Count Estimation | 10,179 | | Date of Issue | 2008-11-21 | | Date of Implementation | 2009-03-01 | | Older Standard (superseded by this standard) | GB/T 4789.27-2003 | | Adopted Standard | AOAC 982.18, MOD | | Regulation (derived from) | National Standard Approval Announcement 2008 No.22 (Total No.135) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies test methods for antibiotic residues in milk. The first method is suitable for standard milk can inhibit Streptococcus thermophilus (Streptococcus thermophilus) antibiotic testing, second law applies to milk can inhibit Bacillus stearothermophilus Khalid variant (Bacillus stearothermophilus var. calidolactis) antibiotic testing can also be used to recover milk, sterilized milk, detection of antibiotics in milk. | GB/T 4789.27-2008: Microbiological examination of food hygiene -- Examination of residue of antibiotics in fresh milk
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Microbiological examination of food hygiene. Examination of residue of antibiotics in fresh milk
ICS 07.100.30
C53
National Standards of People's Republic of China
Replacing GB/T 4789.27-2003
Microbiological examination of food hygiene
Antibiotic residues in milk inspection
Posted 2008-11-21
2009-03-01 implementation
People's Republic of China Ministry of Health
Standardization Administration of China released
Foreword
This revised standard adopts the International Institute analyst (AOAC) AOAC982.18 "liquid milk products in β- amine antibiotic milk qualitative
The color reaction test "(AOACOfficialMethod982.18. Beta-lactamantibioticsinfluidmilkproducts-
Qualitativecolorreactiontests) as the second method.
The main difference compared to the Standard and AOAC982.18 as follows.
--- Modification kits nutrition tablets plus method, directly to the media containing the test strains of Bacillus preparation in a small test tube;
--- The "Note. Occasionally there are special kits batches may require a longer incubation time to complete if every color
10min check color display, and to record the most appropriate incubation time for each batch are required. (Note.
OccasionalykitsofaparticularLotNo. mayrequirealongerincubationtimeforcolorto
fulydevelop. IfCheckcolordevelopmentat10minintervalsandrecordoptimumincu-
bationtimerequiredforeachLotNo. ) "Program was changed to" If the color does not change, shall be re-cultured in a water bath
30min for final observation ";
--- Remove penicillin confirmation procedures.
This standard replaces GB/T 4789.27-2003 "Microbiological examination of food hygiene in antibiotic residues in milk."
This standard compared with GB/T 4789.27-2003 The main changes are as follows.
--- Standard name was changed to "Microbiological examination of food hygiene in antibiotic residues in milk testing";
--- Adds a second method, the original standard thermophilic lactic streptococci method determined as the first method;
--- Added Appendix A "media and reagents."
Appendix A of this standard is a normative appendix.
This standard is proposed and administered by the People's Republic of China Ministry of Health.
This standard by the People's Republic of China Ministry of Health is responsible for interpretation.
This standard is drafted by. Fujian Provincial Center for Disease Control.
Participated in the drafting of this Standard. China Center for Disease Control Nutrition and Food Safety, Chinese Academy of Inspection and Quarantine, Henan
Provincial Center for Disease Control and Prevention, Hubei Center for Disease Control and Prevention.
The main drafters of this standard. horses fly, Guo Yunchang, Li Jin, Tian Wei, Liao Xing Guang, Ma Yi, Tian Jing.
This standard replaces the standards previously issued as follows.
--- GB 4789.27-1984, GB/T 4789.27-1994, GB/T 4789.27-2003.
Microbiological examination of food hygiene
Antibiotic residues in milk inspection
1 Scope
This standard specifies the test methods of antibiotic residues in milk.
The first method Streptococcus thermophilus inhibition
Principle 2
Samples after 80 ℃ sterilization, add Streptococcus thermophilus bacteria. After the incubation period, Streptococcus thermophilus began to proliferate. This time add
The metabolic substrates 2,3,5-triphenyltetrazolium chloride (TTC), if the sample does not contain antibiotics or antibiotic concentration was below the detection limit, addicted
Streptococcus thermophilus will continue to proliferate, reducing TTC become red substance. Conversely, if the sample contains higher than the detection limit of antibacterial agents, the thermophilic
Streptococcus is suppressed, the indicator TTC does not restore, maintain color.
3 equipment and materials
In addition to the microbiological laboratory conventional sterilization and cultivation equipment, other equipment and materials as follows.
3.1 Refrigerator. 2 ℃ ~ 5 ℃, -20 ℃ ~ -5 ℃.
3.2 incubator. 36 ℃ ± 1 ℃.
3.3 lid temperature water bath. 36 ℃ ± 1 ℃, 80 ℃ ± 2 ℃.
3.4 Balance. a sense of the amount of 0.1g, 0.001g.
3.5 sterile pipette. 1mL (with 0.01mL scale), 10.0mL (with 0.1mL scale) or micro pipettes and tips.
3.6 sterile tube. 18mm × 180mm.
3.7 Thermometer. 0 ℃ ~ 100 ℃.
3.8 Vortex Mixer.
4 strains, media and reagents
4.1 bacteria. Streptococcus thermophilus.
4.2 sterilized skim milk. see Section A. Chapter 1.
4.3 4% 2,3,5-triphenyltetrazolium chloride (TTC) solution. See Section A. 2.
4.4 penicillin G reference solution. See Section A. 3.
5 test procedure
Antibiotic residues in milk testing procedures shown in Figure 1.
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