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GB 30604-2015 PDF English


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GB 30604-2015: PDF in English

GB 4789.2-2022 GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA GB 30604-2015 National Food Safety Standard - Food Nutritive Fortifier 1,3-Dioleoyl-2-Palmitoyl Triglyceride ISSUED ON: SEPTEMBER 22, 2015 IMPLEMENTED ON: MARCH 22, 2016 Issued by: National Health and Family Planning Commission of the People's Republic of China Table of Contents 1 Scope ... 3 2 Chemical Name, Molecular Formula, Structural Formula and Relative Molecular Mass ... 3 3 Technical Requirements ... 4 Annex A Inspection Method ... 5 Annex B Typical Chromatogram of Mixed Standard Solution of Glyceryl Triheptadecanoate, 1,3-Dioleoyl-2-Palmitoyl Triglyceride and Palmitoyl Triglyceride and Retention Times of All Components ... 9 National Food Safety Standard - Food Nutritive Fortifier 1,3-Dioleoyl-2-Palmitoyl Triglyceride 1 Scope This standard is applicable to 1,3-dioleoyl-2-palmitoyl triglyceride, a kind of food nutritive fortifier with edible vegetable oil and oleic acid (derived from edible vegetable oils and fats) as raw material and prepared via conversion of lipase catalytic ester for food industry. For preservation purpose, antioxidant may be contained in 1,3-dioleoyl-2-palmitoyl triglyceride. 2 Chemical Name, Molecular Formula, Structural Formula and Relative Molecular Mass 2.1 Chemical name 1,3-dioleoyl-2-palmitoyl triglyceride 2.2 Molecular formula C55H102O6 2.3 Structural formula 2.4 Relative molecular mass 859.39 (in accordance with international relative atomic mass in 2007) Triglyceride base group with same carbon number can be separated from the oil or fat solution via gas chromatography under program-controlled temperature; then it can be confirmed by comparing with the standard triglyceride solution. Carry out quantitative determination by internal standard method, its content is calculated by the triglyceride with carbon number of C52. A.3.2 Reagents and materials A.3.2.1 n-hexane: chromatographically pure. A.3.2.2 Standard product of glyceryl triheptadecanoate: purity ≥ 99%. A.3.2.3 Standard product of 1,3-dioleoyl-2-palmitoyl triglyceride: purity ≥ 99%. A.3.2.4 Standard product of palmitoyl triglyceride: purity ≥ 99%. A.3.2.5 Nitrogen: purity ≥ 99.999%. A.3.3 Apparatus Gas chromatograph: equipped with hydrogen flame ionization detector (FID). A.3.4 Referenced chromatographic condition A.3.4.1 Chromatographic column: 100% dimethyl polysiloxane high-temperature resistant chromatographic column (15m in length, 0.25mm in inner diameter and 0.1μm in film thickness) or the chromatographic column with equivalent performance. A.3.4.2 Sample inlet temperature: 320℃. A.3.4.3 Detector temperature: 370℃. A.3.4.4 Temperature programming: 300℃, keep for 3.0min, and rise to 380℃ at the rate of 20℃/min and keep for 10min. A.3.4.5 Carrier gas: nitrogen, constant voltage mode. A.3.4.6 Sample injection volume: 1.0μL. A.3.4.7 Degree of separation shall be greater than 1.5. A.3.5 Analytical procedures A.3.5.1 Preparation of standard solution A.3.5.1.1 Standard solution of glyceryl triheptadecanoate (2mg/mL): put 100mg standard product of glyceryl triheptadecanoate in a 50mL volumetric flask, add into a proper amount of n-hexane and dissolve it, then dilute to the scale and shake the mixture well. A.3.5.1.2 Standard solution of 1,3-dioleoyl-2-palmitoyl triglyceride (1mg/mL): put 100mg of standard product of 1,3-dioleoyl-2-palmitoyl triglyceride in a 100mL volumetric flask, add a proper amount of n-hexane and dissolve it, then dilute to the scale and shake the mixture well. A.3.5.1.3 Standard solution of palmitoyl triglyceride (1mg/mL): put 100mg standard product of palmitoyl triglyceride in a 100mL volumetric flask, add into proper amount of n-hexane and dissolve it, then dilute to the scale and shake the mixture well. A.3.5.1.4 Preparation of mixed standard solution (0.2mg/mL): transfer 1.0mL of standard solution of glyceryl triheptadecanoate, 2.0mL standard solution of 1,3-dioleoyl-2-palmitoyl triglyceride and 2.0mL of standard solution of palmitoyl triglyceride into a 10mL volumetric flask, add into a proper amount of n-hexane and dilute to the scale, then shake the mixture well. Typical chromatogram of mixed standard solution and retention time of all components are detailed in Annex B. A.3.5.2 Preparation of specimen solution Put 20mg of specimen into a 25mL volumetric flask, then add into a small amount of n-hexane, dissolve the specimen and shake the mixture well. Add into 2mL of internal standard solution of glyceryl triheptadecanoate. Use n-hexane to bring the volume to the scale and shake well. A.3.5.3 Determination Under the specified chromatographic condition, take 1.0μL of standard solution and 1.0μL of specimen solution and inject them into the chromatograph, then record the peak area of component in standard solution and peak area of specimen solution. A.3.6 Result calculation A.3.6.1 The mass fraction (wi) of the content of 1,3-dioleoyl-2-palmitoyl triglyceride and palmitoyl triglyceride in the specimen shall be calculated according to Formula (A.2): Where, Ai — the peak area of 1,3-dioleoyl-2-palmitoyl triglyceride or palmitoyl triglyceride in specimen solution; AR — the peak area of glyceryl triheptadecanoate in chromatogram of specimen solution; ......
Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.