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GB 1886.229-2016 English PDF

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GB 1886.229-2016: Food additive -- Aluminum potassium sulfate
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Standard similar to GB 1886.229-2016

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Basic data

Standard ID GB 1886.229-2016 (GB1886.229-2016)
Description (Translated English) Food additive -- Aluminum potassium sulfate
Sector / Industry National Standard
Classification of Chinese Standard X42
Word Count Estimation 16,197
Date of Issue 2016-08-31
Date of Implementation 2017-01-01
Older Standard (superseded by this standard) GB 1895-2004
Regulation (derived from) Announcement of the State Administration of Public Health and Family Planning 2016 No.11
Issuing agency(ies) National Health and Family Planning Commission of the People's Republic of China, State Food and Drug Administration

GB 1886.229-2016: Food additive -- Aluminum potassium sulfate

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Food safety national standard - Food additive - Potassium aluminum sulfate (also known as potassium alum)) National Standards of People's Republic of China GB 1886.231-2016 National Food Safety Standard Food Additives Nisin Issued on. 2016-08-31 2017-01-01 implementation People's Republic of China National Health and Family Planning Commission released GB 1886.231-2016 National Food Safety Standard Food Additives Nisin

1 Scope

This standard applies to rear by Lactococcus lactis (Lactococcuslactissubsplactis) fermented extract prepared milk food additives Acid nisin. 2 molecular formula, relative molecular mass and structural formula Formula 2.1 C143H230O37N42S7 (NisinA) C141H228O38N41S7 (NisinZ) 2.2 formula NisnA. The first 27 amino acid histidine (His); NisnZ. The first 27 amino acid asparagine (Asn) 2.3 relative molecular mass NisinA. 3354.35 (according to 2013 international relative atomic mass) NisinZ. 3330.31 (according to 2013 international relative atomic mass)

3 Technical requirements

3.1 Sensory requirements Sensory requirements shall comply with the requirements of Table 1. GB 1886.231-2016 Table 1 Sensory requirements Project requires test methods Color light brown to milky white State powder The sample is placed in the appropriate uniform white porcelain plate, observe its color under natural light And state 3.2 Physical and Chemical Indicators Physical and chemical indicators should be consistent with the provisions of Table 2. Table 2. Physical and chemical indicators Item Index Test Method Titer/(IU/mg) ≥ 900 Appendix A A.3 Loss on drying, w /% ≤ 3.0 GB 5009.3 Sodium chloride, w /% ≥ 50.0 GB/T 5009.42 Lead (Pb)/(mg/kg) ≤ 1.0 GB 5009.75 Note. The commercialization of nisin product shall comply with the standards of nisin as a raw material, sodium chloride can be added, and other accessories made of milk solids, In line with its potency claims. 3.3 microbial indicators Microbiological indicators comply with Table 3. Table 3 microbiological indicators Item Index Test Method Cfu/(CFU/g) ≤ 10 GB 4789.2 Coliform/(MPN/g) < 3.0 GB 4789.3 Escherichia coli/(MPN/g) < 3.0 GB 4789.38 Salmonella not detected GB 4789.4 GB 1886.231-2016

Appendix A

Testing method A.1 General Provisions Unless otherwise specified in this standard, the purity of the reagents used should be analytically pure, the standard titration solution, impurity measurement standard solution preparation And products, should be GB/T 601, GB/T 602, the provisions of the preparation of GB/T 603, the test water should be consistent with GB/T 6682 in the three water regulation set. Solution was used in the tests did not indicate what is formulated with solvent, it refers to an aqueous solution. A.2 Identification Test A.2.1 Reagents and materials A.2.1.1 hydrochloric acid solution. 0.02mol/L. A.2.1.2 sodium hydroxide solution. 5mol/L. A.2.1.3 skim milk. fat content of < 1%. A.2.1.4 litmus milk medium. A.2.1.5 detect bacteria. Lactococcus lactis (ATCC11454, NCIMB8586). A.2.2 Analysis step A.2.2.1 Preparation of sample liquid reserves 1g samples taken, dissolved in 1L hydrochloric acid solution, and the solution potency about 1000IU/mL. Stability test A.2.2.2 acid solution Hydrochloric acid solution with the sample stock solution is diluted to about 50IU/mL, thereby preparing a sample solution. The sample solution is boiled for 5min, press titer Determination of the sample solution is measured in boiled Nisin potency. Sample calculation boiled in Nisin potency, efficacy results in its value The (100% ± 5%) within, showed no significant loss of activity. Stability test A.2.2.3 alkali solution Hydrochloric acid solution with the sample stock solution is diluted to about 50IU/mL, thereby preparing a sample solution. The sample solution is boiled for 5min, hydroxide Sodium hydroxide solution was adjusted to pH 11.0 solution was heated to 65 ℃ kept 30min, cool. Then a solution of hydrochloric acid to adjust the pH to 2.0, measured by potency The method of determination of the final solution Nisin potency. In accordance with the above operation will be treated antibacterial activity was almost completely lost. A.2.2.4 different antibacterial substances Nisin discrimination A.2.2.4.1 culture Lactococcus lactis. Lactococcus lactis (job strain) (ATCC11454, NCIMB8586) in sterile skimmed milk Culture, 30 ℃ culture 18h. A.2.2.4.2 or more to prepare a litmus milk medium containing 100mL boiling flask, sterilized at 121 ℃ 15min, 0.1g of The sample was added to the sterile medium litmus milk mix was allowed to stand at room temperature was added 0.1mL detect bacteria after 2h, cultured at 30 ℃ 24h. A.2.2.4.3 detect bacteria (Lactococcus lactis) may be the potency of the sample (about 1000IU/mL) in growth, but not at a similar concentration of its His growth inhibitory substances. GB 1886.231-2016 A.3 Determination of titer A.3.1 Reagents and materials A.3.1.1 nisin standard (potency. 1 × 106IU/g). A.3.1.2 hydrochloric acid solution. 0.02mol/L. A.3.1.3 Tween solution. Tween 20. water = 1/1. A.3.1.4 medium (S1). tryptone, 0.8%; yeast extract 0.5%; glucose, 0.5%; NaCl 0.5%; 0.2% disodium hydrogen phosphate; Joan Powder 1.2% to 1.5%, after sterilization pH6.8 ~ 7.0. A.3.1.5 detect bacteria. yellow Micrococcus (NCIB8166). A.3.2 Analysis step A.3.2.1 detect bacteria (NCIB8166) Training and preparation of the bacterial suspension A.3.2.1.1 detect bacteria culture With a sterile inoculating loop to take a ring detect bacteria (NCIB8166) from glycerol or lyophilized tube tube, seeded on sterile petri dish S1, natural Separation, pick plump, smooth edge of the colony to expand, then the S1 test tube slant for 24h at 30 ℃ incubator, placed in 2 ℃ ~ 5 ℃ refrigerator. Preparation A.3.2.1.2 strain suspension Take detecting bacteria in a refrigerator (NCIB8166), eluted with sterile saline to prepare 108CFU/mL concentration cell suspension, spare. A.3.2.2 Preparation of plates Preparation of S1 media 200mL (in proportion put agar dissolves, followed by adding the components dissolved, after the dissolution of disodium hydrogen phosphate), dried 121 ℃, 20min after sterilization, allowed to cool to about 70 ℃, added 4mL Tween 20 solution, shake well, and so cooled to 50 ℃ ~ 55 ℃ left Right, good suspension is appropriate to add the strain had been prepared to give a final concentration in the medium for the detection of bacteria 1.0 × 106 Ge/mL, shake, pour level Sterilized tablet placed in Once completely solidified, with a diameter of 7mm hole punch, hit the required number of holes in the plate, carefully dig the hole Agar and transferred to a clean workbench hair 1.5h ~ 3.0h (blowing time by humidity in the air on the size, while controlling the temperature of the interior Low, try not to let the detector bacteria growth), after drying, set 2 ℃ ~ 5 ℃ refrigerator to use the next day. A.3.2.3 Preparation of standard solutions Weigh accurately nisin standard (accurate to 0.0001g), was dissolved in hydrochloric acid solution to give a final concentration of 2mg/mL (2000IU/mL), shaken, diluted with hydrochloric acid to 300 times, 600 times, Serve the high and low doses of standard solution. A.3.2.4 Preparation of sample solution Sample volume weighed (accurate to 0.0001g), dissolved in hydrochloric acid, diluted into high, low-dose sample solution, its acid chains Ball streptozotocin content, according to high estimation unit, low-dose and standard solution roughly. A.3.2.5 dropping solution Remove the plate stored in the refrigerator, with a pipette, take 70μL ~ 80μL standard high-dose solution randomly dropped in the plate hole Dropwise six holes, then take 70μL ~ 80μL standard low-dose solution randomly dropped in the rest of the hole with a high dose of the same solution plate 6 GB 1886.231-2016 Hole. Sample solution and standard solution was dropped on the same plate, and its operation is the same standard. A.3.2.6 incubation Solution penetration hole is completely shifted into the 30 ℃ incubator cultured 16h ~ 24h, the inhibition zone diameter measurements. A.3.3 Calculation Results Measuring inhibition zone diameters with calipers and averaged, according to equation (A.1) calculated titer. CSH = CBH × k (XSH XSL) - (XBH XBL) (XSH XBH) - (XSL XBL) (A.1) Where. --- Titer of CSH sample solution, the unit of IU per mg (IU/mg); --- Standard solution of CBH titer units SI units per mg (IU/mg); XSH --- sample solution caused by high-dose inhibition zone diameter in millimeters (mm); XSL --- sample solution due to low-dose inhibition zone diameter in millimeters (mm); XBH --- high doses of standard solution due to inhibition zone diameter in millimeters (mm); XBL --- standard solution due to low-dose inhibition zone diameter in millimeters (mm); k --- high-dose and low-dose concentration ratio. If the estimated value of the sample 90% to 110% range of values, the need to re-estimate the potency of the sample, retest is not measured.

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