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GA/T 1160-2014: DNA extraction from drug plants. Silica method
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DNA extraction from drug plants. Silica method
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GA/T 1160-2014
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Basic data | Standard ID | GA/T 1160-2014 (GA/T1160-2014) | | Description (Translated English) | DNA extraction from drug plants. Silica method | | Sector / Industry | Public Security (Police) Industry Standard (Recommended) | | Classification of Chinese Standard | A92 | | Classification of International Standard | 13.310 | | Word Count Estimation | 6,627 | | Quoted Standard | GB/T 6682-2008 | | Regulation (derived from) | Notice on Publication of Public Safety Industry Standard (Year of 2014); Industry Standard Record Announcement No. 4 of 2015 (No. 184) | | Issuing agency(ies) | Ministry of Public Security | | Summary | This standard specifies the method for the extraction of deoxyribonucleic acid (DNA) from the original plant (cannabis, opium poppy) by the silica method in forensic scientific identification. This standard applies to forensic science in the forensic drug | GA/T 1160-2014: DNA extraction from drug plants. Silica method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
DNA extraction from drug plants.Silica method
ICS 13.310
A92
People's Republic of China Public Security Industry Standards
DNA Extraction common narcotic crops
Silica law
2014-05-09 release
2014-05-09 implementation
People's Republic of China Ministry of Public Security
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard by the National Standardization Technical Committee on Forensic Science and Technology (SAC/TC179) and focal points.
This standard was drafted. Ministry of Public Security Evidence Identification Center.
Drafters of this standard. Pei Li, Tu Zheng, Peng Jianxiong, season security, Donglin Fang, Xu Xiaoyu, Zhang Ying, Feng Tao, Guo Jia, Zhanggui Qin and so on.
DNA Extraction common narcotic crops
Silica law
1 Scope
This standard specifies the use of silica method in forensic identification of extracting DNA from narcotic plants (cannabis, opium poppy) in
(DNA) methods.
This standard applies to DNA extraction of drug plants and their products forensic identification, it can also be used for DNA ordinary plants
extract.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682-2008 Analysis
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Silica law silicamethod
Kind of silica genomic DNA extraction methods of solid phase adsorbents.
Principle 4
Sodium dodecyl sulfate (sodiumdodecylsulfate, SDS) is an ionic surfactant, also protein denaturing agent,
It can dissolve the cell membrane and nuclear membrane protein, so nucleoprotein depolymerization. SDS and proteinase K (PK) joint action to promote protein denaturation, degradation, to crack
Solutions plant cells release the DNA of interest. On the DNA was purified using low pH and high concentrations of chaotropic salts such as guanidine thiocyanate
Under (GuSCN) the presence of a nucleic acid capable of silica-specific binding, the nucleic acid can be high pH at higher temperatures, low salt
Concentration of the solution eluted from the silica characteristics, high quality plant DNA.
5 equipment
5.1 ophthalmic scissors.
5.2 ophthalmic tweezers.
5.3 Vortex.
5.4 high-speed centrifuge (centrifugal force ≥13000g).
5.5 centrifuge tube.
5.6 pipette.
5.7 mortar and pestle organizations such as grinding equipment.
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