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GA/T 1074-2013 English PDF

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GA/T 1074-2013: GC-MS and LC-MS/MS examination methods for γ-hydroxybutyric acid in biological samples
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GA/T 1074-2013English349 Add to Cart 3 days [Need to translate] GC-MS and LC-MS/MS examination methods for γ-hydroxybutyric acid in biological samples GA/T 1074-2013

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Basic data

Standard ID GA/T 1074-2013 (GA/T1074-2013)
Description (Translated English) GC-MS and LC-MS/MS examination methods for ��-hydroxybutyric acid in biological samples
Sector / Industry Public Security (Police) Industry Standard (Recommended)
Classification of Chinese Standard A92
Classification of International Standard 13.310
Word Count Estimation 10,174
Date of Issue 2013-06-28
Date of Implementation 2013-06-28
Quoted Standard GB/T 6682-2008; GA/T 122
Regulation (derived from) Industry standard filing notice 2014 No. 4 (total 172)
Issuing agency(ies) Ministry of Public Security
Summary This standard specifies the gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of ��-hydroxybutyric acid (GHB) in biological samples. This standard applies to qualitativ

GA/T 1074-2013: GC-MS and LC-MS/MS examination methods for γ-hydroxybutyric acid in biological samples


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GC-MS and LC-MS/MS examination methods for γ-hydroxybutyric acid in biological samples ICS 13.310 A92 People's Republic of China Public Security Industry Standards Biological samples γ- hydroxybutyrate Gas Chromatography - Mass spectrometry and liquid chromatography - tandem mass spectrometry test methods Issued on. 2013-06-28 2013-06-28 implementation People's Republic of China Ministry of Public Security

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents. This standard by the National Standardization Technical Committee Technical Criminal Toxicology Analysis Technical Committee (SAC/TC179/SC1) and proposed Centralized. This standard was drafted. the Ministry of Justice of Forensic Science and Technology Institute. The main drafters of this standard. Liu Wei, Shen Min Shen Bao-hua, to flat, Zhuoxian Yi. Biological samples γ- hydroxybutyrate Gas Chromatography - Mass spectrometry and liquid chromatography - tandem mass spectrometry test methods

1 Scope

This standard specifies the biological sample γ- hydroxybutyrate (γ-hydroxybutyricacid, called GHB) gas chromatography - mass spectrometry (GC- MS) and liquid chromatography - tandem mass spectrometry (LC-MS/MS) test method. This standard applies to biological samples (blood, urine, tissue and hair) in GHB qualitative and quantitative analysis.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682-2008 Analysis GA/T 122 toxicological analysis Terminology

3 Terms and Definitions

Terms and definitions GA/T 122 defined apply to this document. Principle 4 4.1 Gas Chromatography - Mass Spectrometry Principle Adding an acidic sample saturated ammonium chloride solution to adjust the pH value of less than 4 to GHB-d6 as the internal standard and extracted with ethyl acetate, and after centrifugation have The organic layer, dry water bath, the residue was subjected to N, O- bis (trimethylsilyl) acetamide difluoro (BSTFA to) or N- tert-butyldimethylsilyl group - N- methyl - after trifluoroacetamide (MTBSTFA) derivatized by gas chromatography - mass spectrometer. After operating in parallel with the reference substance GHB ratio Compared to the retention time and mass spectral characteristic fragment ions for qualitative analysis; based on peak area, internal standard - calibration curve for quantitative analysis. 4.2 Liquid chromatography - tandem mass spectrometry Principle Adding an acidic sample saturated ammonium chloride solution to adjust the pH value of less than 4 to GHB-d6 as the internal standard and extracted with ethyl acetate, and after centrifugation have The organic layer, dry water bath, the residue was dissolved by adding mobile phase, liquid chromatography - tandem mass spectrometer. By parallel operation GHB reference compared to the retention time, and two of the parent ion/ion qualitative analysis; based on peak area, internal standard - calibration curve Statutory amount. 5 reagents, instruments and materials 5.1 Reagents This standard reagents used were of analytical grade unless otherwise provided, a test water is water (see GB/T 6682-2008 specified). a) methanol.

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