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GA/T 765-2020 English PDF

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GA/T 765-2020: Methods for detection of human hemoglobin - Rapid test strip
Status: Valid

GA/T 765: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
GA/T 765-2020English139 Add to Cart 3 days [Need to translate] Methods for detection of human hemoglobin - Rapid test strip Valid GA/T 765-2020
GA 765-2008English199 Add to Cart 3 days [Need to translate] Detection method of human hemolglobin--Rapid test strip Obsolete GA 765-2008

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Basic data

Standard ID GA/T 765-2020 (GA/T765-2020)
Description (Translated English) Methods for detection of human hemoglobin - Rapid test strip
Sector / Industry Public Security (Police) Industry Standard (Recommended)
Classification of Chinese Standard A92
Classification of International Standard 13.310
Word Count Estimation 6,640
Date of Issue 2020-03-12
Date of Implementation 2020-05-01
Older Standard (superseded by this standard) GA 765-2008
Issuing agency(ies) Ministry of Public Security

GA/T 765-2020: Methods for detection of human hemoglobin - Rapid test strip

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(Human hemoglobin detection gold standard reagent strip method) ICS 13.310 A92 People's Republic of China Public Safety Industry Standard Replace GA 765-2008 Human hemoglobin test Gold-labeled reagent strip method 2020-03-12 released 2020-05-01 Implementation Issued by the Ministry of Public Security of the People's Republic of China

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces GA 765-2008 "Human Hemoglobin Detection Gold Standard Reagent Strip Method". Compared with GA 765-2008, the main technical changes are as follows. ---Revised as a recommended standard (see cover, cover of.2008 edition); ---Added the requirements for pure water (see 4.1). This standard was proposed and managed by the Forensic Examination Subcommittee of the National Criminal Technology Standardization Technical Committee (SAC/TC179/SC6). This standard was drafted by. Material Evidence Appraisal Center of the Ministry of Public Security, Heilongjiang Provincial Public Security Department. The main drafters of this standard. Wen Peizhong, Bai Xue, Meng Qingzhen, Euro, Zhang Da'an, Liu Yihui, Li Xiaoping, Zhu Aimin, Li Peng, Liu Xudan, Chang Caiqin and Zhang Yinglan. The previous versions of the standard replaced by this standard are as follows. ---GA 765-2008. Human hemoglobin test Gold-labeled reagent strip method

1 Scope

This standard specifies the testing principles and material standards for rapid testing of human hemoglobin (Hb) through gold standard test strips in forensic doctors’ physical evidence testing. Precautions in preparation, operation method, result judgment and inspection. This standard is applicable to forensic scientific inspections to determine whether the test materials identified by the inspection contain human hemoglobin.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB/T 6682-2008 Analysis laboratory water specifications and test methods GA 476 Human Hemoglobin Gold Standard Test Reagent Strip

3 Principle

The basic principle of the anti-human hemoglobin gold-labeled reagent strip method is the gold-labeled double antibody sandwich method. The anti-human hemoglobin gold-labeled reagent strip is divided into three areas, namely the sample area, the reaction area and the adsorption area. Gold labeled monoclonal adsorbed on the sample area After the anti-human hemoglobin antibody reacts with the human blood stain soaking solution (containing human hemoglobin), it forms a gold-labeled antigen-antibody complex, and sends it to the reagent strip The adsorption zone diffuses. When the complex diffuses to the reaction zone, it binds to another monoclonal anti-human hemoglobin antibody adsorbed in this zone (linear). The original antibody complex gathers there, forming a purple-red line (positive reaction line). The excess complexes continue to diffuse to the adsorption zone and adsorb with the reaction zone The other antibody (usually goat anti-mouse IgG) combined, also formed a purple-red line (quality control line). If the soaking solution does not contain human hemoglobin, the antigen-antibody complex cannot be formed in the sample area, and the gold-labeled anti-human blood diffused to the reaction area The red protein antibody cannot bind to another monoclonal anti-human hemoglobin antibody adsorbed in this area, and there is no purple-red linear band formed. When it continues to diffuse to the adsorption zone, it can combine with another adsorbed goat anti-mouse IgG in the reaction zone to form a purple-red line (quality control line).

4 Reagents and equipment

4.1 Reagents The reagents include. a) Human hemoglobin gold standard test kit (in line with the requirements of GA 476); b) Pure water (conforms to the tertiary water requirements specified in GB/T 6682-2008). 4.2 Equipment Equipment includes. a) ophthalmic scissors; b) Ophthalmic forceps;

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